|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Liver fat deposition and mitochondrial dysfunction in morbid obesity:An approach combining metabolomics with liver imaging and histology显示文摘AIM: To explore the usefulness of magnetic resonance imaging(MRI) and spectroscopy(MRS) for assessment of non-alcoholic fat liver disease(NAFLD) as compared with liver histological and metabolomics findings. METHODS: Patients undergoing bariatric surgery following procedures involved in laparoscopic sleeve gastrectomy were recruited as a model of obesityinduced NAFLD in an observational, prospective, singlesite, cross-sectional study with a pre-set duration of 1 year. Relevant data were obtained prospectively and surrogates for inflammation, oxidative stress and lipid and glucose metabolism were obtained through standard laboratory measurements. To provide reliable data from MRI and MRS, novel procedures were designed to limit sampling variability and other sources of error using a 1.5T Signa HDx scanner and protocols acquired from the 3D or 2D Fat SAT FIESTA prescription manager. We used our previously described 1H NMRbased metabolomics assays. Data were obtained immediately before surgery and after a 12-mo period including histology of the liver and measurement of metabolites. Values from 1H NMR spectra obtained after surgery were omitted due to technical limitations.RESULTS: MRI data showed excellent correlation with the concentration of liver triglycerides, other hepatic lipid components and the histological assessment, w h i c h e xc l u d e d t h e p r e s e n c e o f n o n-a l c o h o l i c steatohepatitis(NASH). MRI was sufficient to follow up NAFLD in obese patients undergoing bariatric surgery and data suggest usefulness in other clinical situations. The information provided by MRS replicated that obtained by MRI using the-CH3 peak(0.9 ppm), the-CH2- peak(1.3 ppm, mostly triglyceride) and the-CH=CH- peak(2.2 ppm). No patient depicted NASH. After surgery all patients significantly decreased their body weight and steatosis was virtually absent even in patients with previous severe disease. Improvement was also observed in the serum concentrations of selected variables. The most relevant findings using metabolomics indicate increased levels of triglyceride and monounsaturated fatty acids in severe steatosis but those results were accompanied by a significant depletion of diglycerides, polyunsaturated fatty acids, glucose-6-phosphate and the ATP/AMP ratio. Combined data indicated the coordinated action on mitochondrial fat oxidation and glucose transport activity and may support the consideration of NAFLD as a likely mitochondrial disease. This concept may helpto explain the dissociation between excess lipid storage in adipose tissue and NAFLD and may direct the search for plasma biomarkers and novel therapeutic strategies. A limitation of our study is that data were obtained in a relatively low number of patients.CONCLUSION: MRI is sufficient to stage NAFLD in obese patients and to assess the improvement after bariatric surgery. Other data were superfluous for this purpose. | Nahum Calvo Raúl Beltrán-Debón Esther Rodríguez-Gallego Anna Hernández-Aguilera Maria Guirro Roger Mariné-Casadó Lidón Millá Josep M Alegret Fàtima Sabench Daniel del Castillo María Vinaixa Miguelàngel Rodríguez Xavier Correig Roberto García-álvarez Javier A Menendez Jordi Camps Jorge Joven | 2015 | World Journal of Gastroenterology2015，21，24： | 2 |
| 2 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage 显示文摘 | E1 Barbri N Amari A Vinaixa M | 2008 | Sensors and Actuators B Chemical2008，128，1： | 1 |
| 3 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage显示文摘 | Barbfi N E Amafi A Vinaixa M | 2007 | Sensors and Actuators B:Chemical2007，128，： | 1 |
| 4 | Metabolic phenotyping of genetically modified mice: An NMR metabonomic approach显示文摘 | Rull A Vinaixa M Angel Rodriguez M | 2009 | Biochimie2009，91，8： | 1 |
| 5 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage显示文摘 | BARBRI N E AMARI A VINAIXA M BOUCHIKHI B CORREIG X LLOBET E | 2007 | Sensors and Actuators B-Chemical2007，128，1： | 1 |
| 6 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage 显示文摘 | E1 Barbri N Amari A Vinaixa M | 2007 | Sensors and Actuators B : Chemical2007，128，1： | 1 |
| 7 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage显示文摘 | Barbri N E Amari A Vinaixa M | 2007 | Sensors and Actuators B:Chemical2007，128，1： | 1 |
| 8 | Metabolicphenotyping of genetically modified mice: An NMR metabonomicapproach显示文摘 | Rull A Vinaixa M Angel Rodriguez M | 2009 | Biochimie2009，91，8： | 1 |
| 9 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage 显示文摘 | E1 Barbri N Amari A Vinaixa M | 2007 | Sensors and Actuators B : Chemical2007，128，1： | 1 |
| 10 | Efficient feature selection for mass spectrometry based electronic nose ap- plicatons 显示文摘 | Llobet E Gualdron O Vinaixa M | 2007 | Chemometrics and Intelligent Labor-atory Systems2007，85，2： | 1 |
| 11 | Metabolic phenotyping of genetically modified mice: An NMR metabonomic approach 显示文摘 | RULL A VINAIXA M ANGEL RODRtGUEZ M | 2009 | See Comment PubMed Commons Biochim2009，91，8： | 1 |
| 12 | Building of a metal oxide gas sensor-based electronic nose to assess the fresh-ness of sardines under cold storage显示文摘 | Barbri N E Amari A Vinaixa M | | 0，，： | 1 |
| 13 | Building of a metal oxide gas sensor-based electronic nose to assess the fresh- ness of sardines under cold storage 显示文摘 | E1 Barbri N Amari A Vinaixa M | 2007 | Sensors and Actu- ators B : Chemical2007，128，1： | 1 |
| 14 | Metabolornics reveals reduction of metabolic oxidation in women with polycystic ovary syndrome after pioglitazone-flutamide-rnet- forrnin polytherapy显示文摘 | Vinaixa M Rodriguez MA Sarnino S | 2011 | PLoS One2011，6，29： | 1 |
| 15 | A fuzzy ARTMAP-and PLS-based MS e-nose for the qualitative and quantitative assessment of ran- cidity in crisps 显示文摘 | Vinaixa M Llobet E Brezmes J | 2005 | Sensors and Actuators B: Chemical2005，106，2： | 1 |
| 16 | Building of a metal oxide gas sensor-based electronic nose to assess the freshness of sardines under cold storage 显示文摘 | Barbri N E I Amari A Vinaixa M Bouchikhi B Correig X Llobet E | 2007 | Sensors and Actuators B : Chemical2007，128，1： | 1 |
| 17 | Metabolomics reveals reduction of metabolic oxidation in women with polycystic ovarysyndrome after pioglitazone-flutamide-metformin polytherapy 显示文摘 | Vinaixa M Rodriguez MA Samino S | 2011 | PLoS One2011，6，29： | 1 |
| 18 | Metabolomics re- veals reduction of metabolic oxidation in women with polycys- tic ovary syndrome after pioglitazone-flutamide-metformin polytherapy显示文摘 | Vinaixa M Rodriguez MA Samino S | 2011 | PLoS One2011，6，29： | 1 |
| 19 | SYNBIOCHEM Synthetic Biology Research Centre, Manchester-A UK foundry for fine and speciality chemicals production显示文摘The UK Synthetic Biology Research Centre,SYNBIOCHEM,hosted by the Manchester Institute of Biotechnology at the University of Manchester is delivering innovative technology platforms to facilitate the predictable engineering of microbial bio-factories for fine and speciality chemicals production.We provide an overview of our foundry activities that are being applied to grand challenge projects to deliver innovation in bio-based chemicals production for industrial biotechnology. | Le Feuvre RA Carbonell P Currin A Dunstan M Fellows D Jervis AJ Rattray NJW Robinson CJ Swainston N Vinaixa M Williams A Yan C Barran P Breitling R Chen GG Faulon JL Goble C Goodacre R Kell DB Micklefield J Scrutton NS Shapira P Takano E Turner NJ | 2016 | Synthetic and Systems Biotechnology2016，1，4： | 1 |
| 20 | Efficient feature selec-tion for mass spectrometry based electronic nose applications显示文摘 | Llobet E Gualdro 0 Vinaixa M | 2007 | Chemometrics and Intelligent Laboratory Systems2007，85，2： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员