| 1 | Enterolactone modulates the ERK/NF-κB/Snail signaling pathway in triple-negative breast cancer cell line MDA-MB-231 to revert the TGF-β-induced epithelial–mesenchymal transition显示文摘Objective:Triple-negative breast cancer(TNBC)is highly metastatic,and there is an urgent unmet need to develop novel therapeutic strategies leading to the new drug discoveries against metastasis.The transforming growth factor-β(TGF-β)is known to promote the invasive and migratory potential of breast cancer cells through induction of epithelial–mesenchymal transition(EMT)via the ERK/NF-κB/Snail signaling pathway,leading to breast cancer metastasis.Targeting this pathway to revert the EMT would be an attractive,novel therapeutic strategy to halt breast cancer metastasis.Methods:Effects of enterolactone(EL)on the cell cycle and apoptosis were investigated using flow cytometry and a cleaved caspase-3 enzyme-linked immunosorbent assay(ELISA),respectively.Effects of TGF-βinduction and EL treatment on the functional malignancy of MDA-MB-231 breast cancer cells were investigated using migration and chemo-invasion assays.The effects of EL on EMT markers and the ERK/NF-κB/Snail signaling pathway after TGF-βinduction were studied using confocal microscopy,quantitative reverse transcription polymerase chain reaction(q RT-PCR),Western blot,and flow cytometry.Results:Herein,we report that EL exhibits a significant antimetastatic effect on MDA-MB-231 cells by almost reverting the TGF-β-induced EMT in vitro.EL downregulates the mesenchymal markers N-cadherin and vimentin,and upregulates the epithelial markers E-cadherin and occludin.It represses actin stress fiber formation via inhibition of mitogen-activated protein kinase p-38(MAPK-p38)and cluster of differentiation 44(CD44).EL also suppresses ERK-1/2,NF-κB,and Snail at the m RNA and protein levels.Conclusions:Briefly,EL was found to inhibit TGF-β-induced EMT by blocking the ERK/NF-κB/Snail signaling pathway,which is a promising target for breast cancer metastasis therapy. | Aniket V.Mali Asavari A.Joshi Mahabaleshwar V.Hegde Shivajirao S.Kadam | 2018 | Cancer Biology & Medicine2018，15，2： | 6 |
| 2 | 毛圈织物疵点质量检测优化分析显示文摘织物检验部门通过确保输送无质量问题的产品为企业建立良好的声誉,在纺织工业中起着至关重要的作用。因此,基于质量优先的织物制造必须采用电子控制的检查系统。长期以来,织物疵点的检测过程是通过肉眼观察来完成,事实证明,这种检测方法有缺陷,且成本高。为降低织物的次品率而对不同批次的织物进行了检验,然后在织机上优化织造参数设定,这有助于减少41%的织物缺经﹑40%的跳花和33%的抽毛等疵点率。 | V.Mali R.Turukmane 李华(译) 孟粉叶(校) | 2021 | 国际纺织导报2021，49，2： | 0 |
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