| 1 | 基于油藏特征的完井方法显示文摘通过优化完井,无需动用管柱,可提高措施效率、开采多个油气藏及多层非均质砂岩油藏,从而控制开发成本并提高采收率。印尼苏门答腊Jabung区块开发井钻遇三类典型油气藏:气顶油环油气藏、多油气藏及多砂岩非均质油藏。根据各类油气藏的特点,采用针对性的完井方法,优化的完井方法利用常规完井技术和井下工具实现单井一次完井,无需复杂的再完井措施,可同时或接替生产多个油气藏。此外,针对非均质多砂岩特点,应用可控的分层注水技术,可提高油藏采收率。上述完井方法已成功应用于不同类型油藏中,为油气水分布复杂的小断块油田开发过程中控制操作成本、提高开发井的整体效益提供了借鉴。 | 吕晓光 张学文 刘永喜 Bradford W Sincock Budi Tyas Utomo | 2008 | 石油勘探与开发2008，35，4： | 5 |
| 11 | Mechanism of spinal cord injury regeneration and the effect of human neural stem cells-secretome treatment in rat model显示文摘BACKGROUND Globally,complete neurological recovery of spinal cord injury(SCI)is still less than 1%,and 90%experience permanent disability.The key issue is that a pharmacological neuroprotective-neuroregenerative agent and SCI regeneration mechanism have not been found.The secretomes of stem cell are an emerging neurotrophic agent,but the effect of human neural stem cells(HNSCs)secretome on SCI is still unclear.AIM To investigate the regeneration mechanism of SCI and neuroprotective-neuroregenerative effects of HNSCs-secretome on subacute SCI post-laminectomy in rats.METHODS An experimental study was conducted with 45 Rattus norvegicus,divided into 15 normal,15 control(10 mL physiologic saline),and 15 treatment(30μL HNSCssecretome,intrathecal T10,three days post-traumatic).Locomotor function was evaluated weekly by blinded evaluators.Fifty-six days post-injury,specimens were collected,and spinal cord lesion,free radical oxidative stress(F2-Isoprostanes),nuclear factor-kappa B(NF-κB),matrix metallopeptidase 9(MMP9),tumor necrosis factor-alpha(TNF-α),interleukin-10(IL-10),transforming growth factor-beta(TGF-β),vascular endothelial growth factor(VEGF),B cell lymphoma-2(Bcl-2),nestin,brain-derived neurotrophic factor(BDNF),glial cell line-derived neurotrophic factor(GDNF)were analyzed.The SCI regeneration mechanism was analyzed using partial least squares structural equation modeling(PLS SEM).RESULTS HNSCs-secretome significantly improved locomotor recovery according to Basso,Beattie,Bresnahan(BBB)scores and increased neurogenesis(nestin,BDNF,and GDNF),neuroangiogenesis(VEGF),anti-apoptotic(Bcl-2),anti-inflammatory(IL-10 and TGF-β),but decreased proinflammatory(NF-κB,MMP9,TNF-α),F2-Isoprostanes,and spinal cord lesion size.The SCI regeneration mechanism is valid by analyzed outer model,inner model,and hypothesis testing in PLS SEM,started with pro-inflammation followed by anti-inflammation,anti-apoptotic,neuroangiogenesis,neurogenesis,and locomotor function.CONCLUSION HNSCs-secretome as a potential neuroprotective-neuroregenerative agent for the treatment of SCI and uncover the SCI regeneration mechanism. | I Nyoman Semita Dwikora Novembri Utomo Heri Suroto | 2023 | World Journal of Orthopedics2023，14，2： | 1 |
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