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| 1 | Abnormal expression of hepatoma- derived γ-glutamyltransferase subtyping and its early alteration for carcinogenesis of hepatocytes显示文摘BACKGROUND: Although the hepatoma-specific band of gamma-glutamyltransferase ( GGT ) is a highly sensitive marker in diagnosis of hepatocellular carcinoma, the kine- tic expression and the early alterations of GGT in the deve- lopment of hepatoma remain unclear. In this study, we in- vestigated the expression and the alterations of GGT multi- ple molecular forms in hepatotumorigenesis. METHODS: The expression of GGT in a chemically in- duced hepatocarcinogenesis model was examined by giving 0. 05% of 2-fluoenylacetamide in diet for 12 weeks. The ex- pression levels of total RNA and GGT, and the changes of liver pathology, GGT multiple molecular forms and sugar- chain heterogeneity were investigated at the different stages of rat hepatoma development. RESULTS: Pathological examination and biochemical ana- lysis found that liver GGT was over-expressed and secreted into blood during canceration. Serum total GGT and liver GGT specific activities (IU/g) including soluble and mem- brane-combined GGT were significantly higher (P <0.05) in experimental groups than those in control group, respec- tively. A highly positive correlation was found between to- tal GGT activities and total RNA levels (r =0.90, P <0.05) of the liver. Both were higher six weeks later than before. Con A-non-reactive-GGT was increased consistantly dur- ing the development of rat hepatoma. GGT multiple mo- lecular forms in the liver and sera of experimental rats showed that fetal liver-type GGT bands were associated with the development of hepatoma. CONCLUSIONS: Fetal liver-type GGT in sera and the liver of rats is closely related to hepatotumorigenesis. It can be used as a sensitive enzymatic marker for the early diagnosis of liver cancer. | Deng-Fu Yao, Zhi-Zhen Dong, Deng-Bing Yao, Xin-Hua Wu, Wei Wu, Li-Wei Qiu, Hong-Mei Wang and Xian-Yong Meng Nantong, China Research Center of Clinical Molecular Biology, Affilia- ted Hospital of Nantong University, Nantong 226001 , China Insti- tute of Neuroscicnces, Nantong University, Nantong 226001 , China | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 17 |
| 2 | Analysis of tandem repeats in the genome of Chinese shrimp Fenneropenaeus chinensis显示文摘Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of Chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2159 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 20.6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2% of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42%. In decreasing order, the 20 most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (274), AAT (92), A (56), AAG (28), ATC (27), ATAG (27), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them. | KONG Jie1 & GAO Huan2,3 1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research Insti- tute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China | 2005 | Chinese Science Bulletin2005，50，14： | 7 |
| 3 | Seven microcystins from Microcystis waterbloom in Lake Dalai,China显示文摘Seven types of microcystins,isolated from Microcystis waterbloom in Lake Dalai,were characterized.The major toxins:MCYST-LR,MCYST-RR,[D-Asp3]MCYST-LR and[Dha7]MCYST-LR were identified by high performance liquid chromatography(HPLC),as com-pared with the authentic microcystins.The minor toxins:MCYST FR,[L-Mser7]MCYST-LRand an unknown MCYST which was most likely to be MCYST-(H4)YR were identified with frit-fast atom bombardment liquid chromatography/mass spectrometry(Frit-FAB LC/MS)and aminoacid analysis.The toxigenic diversity in blue-green algae(cyanobacteria)was discussed. | He Zhenrong He Jiawan Yu Minjuan Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,ChinaQiao Mingye Shen Zhi Wu Liandi Inner Mongolia Environmental Science Research Instìtute,Huhehote 010010,ChinaWu Suozhu Guo Yunfeng Hulunbeer League Environmental Monitoring Station,Inner Mogolia 021008,ChinaFumio Kondo Aichi Prefectural Institute of Public Health,Tsuji-machi,Kita-ku,Nagoya 462,JapanKen-ichi Harada Faculty of Pharmacy,Meijo University,Tempaku,Nagoya 468,Japan | 1997 | Journal of Environmental Sciences1997，9，1： | 6 |
| 4 | Selenocysteine tRNAs as Central Components of Selenoprotein Biosynthesis in Eukaryotes显示文摘Selenocysteine (Sec) tRNAs serve as carrier molecules for the biosynthesis of Sec from serine and to donate Sec to protein in response to specific UGA codons. In this study, we describe the current status of Sec tRNAs in higher animals and further we exarnine: (i) the Sec tRNA population in Drosophila; (ii) transcription of the Sec tRNA in vivo (in Xenopus oocytes) and in vitro (in Xenopus oocyte extracts); (iii) the effect of selenium on the Sec tRNA population in various rat tissues following replenishment of extremely selenium deficient rats with this element; and (iv) the biosynthesis of the modified bases on Sec tRNA in Xenopus | SANG ICK PARK JIN Mo PARK HAROLD S. CHITTUM EUN SUNG YANG BRADLEY A. CARLSON BYEONG JAE LEE AND DOLPH L. HATFIELD(Laboratory of Experimental Chrcinogenesis, National Cancer Insti tute, National Institutes of Health, Bethesda, MD 20892USA Laboratory o | 1997 | Biomedical and Environmental Sciences1997，10，2： | 2 |
| 5 | The immunology of cutaneous DNA immunization显示文摘 | Tuting T | 1999 | Curr Opin Mol Ther1999，1，2： | 1 |
| 6 | An IFN-associated cytotoxic cellular immune response against viral, self-, or tumor antigens is a common pathogenetic feature in 'interface dermatitis' 显示文摘 | Wenzel J Tuting T | 2008 | J Invest Dermatol2008，128，10： | 1 |
| 7 | A comparison of two types of dendritic cell as adjuvants for the induction of melanoma-specific T-cell responses in humans following intranodal injection 显示文摘 | Jonulelt H Gieseeke-Tuettenberg A Tuting T | 2001 | lnt J Cancer2001，93，2： | 1 |
| 8 | Minimal residual disease assessed by multiparameter flow cytometry in multiple myeloma: impact on outcome in the Medical Research Council Myeloma IX Study显示文摘 | Rawstron AC Child JA de Tute RM | 2013 | J Clin Oncol2013，31，20： | 1 |
| 9 | Induction of tumor antigen specific immunity using plasmid DNA immunization in mice显示文摘 | Tuting V Gambotto A DeLeo A | 1999 | Cancer Gene Therapy1999，6，： | 1 |
| 10 | Autologous human monocyte-derived dendritic cells genetically modified to express melanoma antigens elicit primary cytotoxic T cell response in vitro: enhancement by cotransfection of genes encoding the Th1-biasing cytokines IL-12 and IFN-(显示文摘 | Wilson CC Mrtin DM | 1998 | J Immunol1998，160，： | 1 |
| 11 | Induction of tumor antigen specific immunity using plasmid DNA immunization in mice显示文摘 | TUTING T GAMBOTTO A DELEO A | 1999 | Cancer Gene Ther1999，6，： | 1 |
| 12 | Primary plasmacytoma of the skin显示文摘 | Tuting T Bork K | 1996 | J Am Acad Dermatol1996，34，22： | 1 |
| 13 | Myocardial injury in critically ill patients-a frequently unrecognized complication显示文摘 | Guest TM Raman thaw AV Tute PG | 1995 | JAMA1995，273，21： | 1 |
| 14 | A comparison of two types of dendritic cell as adjuvants for the induction of mel- anoma-specific T-cell responses in humans following intranodal injection显示文摘 | Jonuleit H Giesecke-Tuettenberg A Tuting T | 2001 | Int J Cancer2001，93，2： | 1 |
| 15 | A comparison of two types of dendritic cell as adjuvants for the induction of melano- ma - specific T - cell responses in humans following intranodal injec- tion显示文摘 | Jonuleit H Giesecke - Tuettenberg A Tuting T | 2008 | Int JCancer2008，93，2： | 1 |
| 16 | Primary plasmacytoma of the skin 显示文摘 | Tuting T Bork K | 1996 | J Am Acad Dermatol1996，34，22： | 1 |
| 17 | Autologous human monocyte-derived dendritic cells genetically modified to express melanoma antigens elicit primary cytotoxic T cell responses in vito:enhancement by cotransfection of genes encoding the Th1-biasing cytokiness IL-12 and IFN-a显示文摘 | Wilson CC Martin DM | 1998 | J Immunol1998，160，30： | 1 |
| 18 | Autologus human monocyte-derived dendritic cells genetically modified to express melanoma antigens elicit primary cytotoxic Tcell responses In Vitro :enhancement by cotransfection of genes encoding the Th1biasing cytokings IL-12 and IFN- α显示文摘 | Tuting T Wilson CC Martin DM | 1998 | J Immuuol1998，160，3： | 1 |
| 19 | HIV 1-specific CTL responses primed in vitro by blood-derived dendritic cells and Th1-biasing cytokines 显示文摘 | OLSON WC TUTING T | 1999 | J Immunol1999，162，5： | 1 |
| 20 | A single-tube six- colour flow cytometry screening assay for the detection of minimal residual disease in myeloma显示文摘 | de Tute RM Jaek AS Child JA | 2007 | Leukemia2007，21，9： | 1 |
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