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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Eigenfaces for Recognition显示文摘 | Turk M Pentland A | 1991 | Journal of Cognitive Neurosicence1991，3，1： | 1 |
| 3 | Eigenfaces for recognition 显示文摘 | Turk M Pentland A | 1991 | J of Cognitive Neuroscience1991，3，1： | 1 |
| 4 | Eigenfaces for face recognition显示文摘 | TURK M PENTLAND A | 1991 | Journal Cognitive Neuroscience1991，3，1： | 1 |
| 5 | Eigen faces for recognition显示文摘 | TURK M PENTLAND A | | 0，，01： | 1 |
| 6 | Eigenfaces for recognition显示文摘 | Turk M Pentland A | 1991 | Journal of Cognitive Neuroscienee1991，3，1： | 1 |
| 7 | Eigenfaces for recognition 显示文摘 | TURK M PENTLAND A | 1991 | Journal of Cognitive Neuroscience1991，3，1： | 1 |
| 8 | Inhibitory effects of aqueous extracts of black mustard on germination and growth of alfalfa显示文摘 | Turk M A SHATNAWI M K TAWAHA A M | 2003 | Weed Biology and Management2003，，3： | 1 |
| 9 | Inhibitory effects of aqueous extracts of black mustard on germination and growth of lentil显示文摘 | Turk M A Tawaha A M | 2002 | Pakistan Journal of Agronomy2002，1，1： | 1 |
| 10 | Laparoscopic dismembered pyeloplasty-the method of choice in the presence of an enlarged renal pelvis and crossing vessels显示文摘 | Turk I A Davis J W Winkelmann B | 2002 | Eur Urol2002，42，： | 1 |
| 11 | Effects of board and ownership structure on corporate R&D strategy显示文摘 | Baysinger B D Kosnik R D Turk T A | 1991 | Academy of Management Journal1991，34，： | 1 |
| 12 | Eigenfaces for Recognition显示文摘 | Turk M Pentland A | 1991 | Journal of Cognitive Neuroscience1991，3，1： | 1 |
| 13 | Eigenfaces for recognition显示文摘 | Turk M Pentland A | 1991 | Journal Cognitive Neuro-science1991，3，1： | 1 |
| 14 | Eigenfaces for Recognition显示文摘 | Turk M Pentland A | 1991 | Cognitive Neuro Science1991，3，1： | 1 |
| 15 | Eigenface for Recognition显示文摘 | Turk M Pentland A | 1991 | Journal of Cognitive Neuoscience1991，3，1： | 1 |
| 16 | Eigenfaces for recognition 显示文摘 | Turk M Pentland A | 1991 | Journal of Cognitive Neuroscience1991，3，1： | 1 |
| 17 | Eigenface for recognition显示文摘 | Turk M Pentland A | | 0，，01： | 1 |
| 18 | Eigenfaces for Recognition显示文摘 | Turk M Pentland A | | 0，，01： | 1 |
| 19 | Optimization of a self antigen for presentation of multiple epitopes in cancer immunity显示文摘 | GUEVARA PATINO J A ENGELHORN M E TURK M J | 2006 | J Clin Invest2006，116，5： | 1 |
| 20 | Utility of computed tomog- raphy perfusion in detection of cerebral vasospasm in patients with subaraehnoid hemorrhage显示文摘 | Mottaldaar R Rowley HA Turk A | 2006 | Neurosurg Focus2006，21，3： | 1 |
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