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| 1 | Mechanisms of regulation of PFKFB expression in pancreatic and gastric cancer cells显示文摘Enzymes 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 and-4(PFKFB-3 and PFKFB-4)play a significant role in the regulation of glycolysis in cancer cells as well as its proliferation and survival.The expression of these mRNAs is increased in malignant tumors and strongly induced in different cancer cell lines by hypoxia inducible factor(HIF)through active HIF binding sites in promoter region of PFKFB-4 and PFKFB-3 genes.Moreover,the expression and hypoxia responsibility of PFKFB-4 and PFKFB-3 was also shown for pancreatic(Panc1,PSN-1,and MIA PaCa-2)as well as gastric(MKN45 and NUGC3)cancer cells.At the same time,their basal expression level and hypoxia responsiveness vary in the different cells studied:the highest level of PFKFB-4 protein expression was found in NUGC3 gastric cancer cell line and lowest in Panc1 cells,with a stronger response to hypoxia in the pancreatic cancer cell line.Overexpression of different PFKFB in pancreatic and gastric cancer cells under hypoxic condition is correlated with enhanced expression of vascular endothelial growth factor(VEGF)and Glut1 mRNA as well as with increased level of HIF-1αprotein.Increased expression of different PFKFB genes was also demonstrated in gastric,lung,breast,and colon cancers as compared to corresponding nonmalignant tissue counterparts from the same patients,being more robust in the breast and lung tumors.Moreover,induction of PFKFB-4 mRNA expression in the breast and lung cancers is stronger than PFKFB-3mRNA.The levels of both PFKFB-4 and PFKFB-3 proteins in non-malignant gastric and colon tissues were more pronounced than in the non-malignant breast and lung tissues.It is interesting to note that Panc1and PSN-1 cells transfected with dominant/negative PFKFB-3(dnPFKFB-3)showed a lower level of endogenous PFKFB-3,PFKFB-4,and VEGF mRNA expressions as well as a decreased proliferation rate of these cells.Moreover,a similar effect had dnPFKFB-4.In conclusion,there is strong evidence that PFKFB-4 and PFKFB-3 isoenzymes are induced under hypoxia in pancreatic and other cancer cell lines,are overexpressed in gastric,colon,lung,and breast malignant tumors and undergo changes in their metabolism that contribute to the proliferation and survival of cancer cells.Thus,targeting these PFKFB may therefore present new therapeutic opportunities. | Oleksandr H Minchenko Katsuya Tsuchihara Dmytro O Minchenko Andreas Bikfalvi Hiroyasu Esumi | 2014 | World Journal of Gastroenterology2014，20，38： | 17 |
| 2 | FoxO3a-dependent regulation of puma in response to cytokine/growth factor withdrawal显示文摘 | You H Pellegrini M Tsuchihara K | 2006 | J Exp Med2006，203，7： | 1 |
| 3 | Nonviral retrograde gene transfer of human hepatocyte growth factor improves neuropathic pain-related phenomena in rats 显示文摘 | Tsuchihara T Ogata S Nemoto K | 2009 | Mol Ther2009，17，1： | 1 |
| 4 | FOXO3a-dependent regulation of Puma in response to cytokine/growth factor withdrawal 显示文摘 | You H Pellegrini M Tsuchihara K | 2006 | JExpMed2006，203，7： | 1 |
| 5 | Autophagy is acti-vated in colorectal cancer cells and contributes to the tol-erance to nutrient deprivation显示文摘 | Sato K Tsuchihara K Fujii S | | 0，，20： | 1 |
| 6 | Comparison of the images in virtual bronchoscopy under different conditions显示文摘 | Sagawa M Usuda K Tsuchihara K | 2008 | Kyobu Geka2008，61，2： | 1 |
| 7 | Irradiated pancreatic cancer cells undergo both apoptosis and necrosis, and could be phagocytized by dendritic cells显示文摘 | Shimamura H Sunamura M Tsuchihara K | 2005 | Eur Surg Res2005，37，4： | 1 |
| 8 | Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation 显示文摘 | Sato K Tsuchihara K Fujii S | 2007 | Cancer Res2007，67，20： | 1 |
| 9 | Molecular cloning and characterization of cDNAs encoding dopamine receptor-1 and-2 from brain-suboesophageal ganglion of the silkworm,Bombyx mori显示文摘 | Mitsumasu K Ohta H Tsuchihara K Asaoka K Ozoe Y Niimi T Yamashita O Yaginuma T | | 0，，02： | 1 |
| 10 | Hepatitis C virus core protein enhances the activation of the transcription factor,Elk1,in response to mitogenic stimuli显示文摘 | TSUCHIHARA K HIJIKATA M | 2001 | Hepatology2001，33，： | 1 |
| 11 | Hepatitis C virus core protein enhances the activation of the transcription factor, Elk1,in response to mitogenic stimuli显示文摘 | Fukuda K Tsuchihara K Hijikata M | 2001 | Hepatology2001，33，3： | 1 |
| 12 | Autophagy and cancer:dynamism of the metabolism of tumor cells and tissues显示文摘 | Tsuchihara K Fujii S Esumi H | 2009 | Can Lett2009，278，2： | 1 |
| 13 | Autophagy and cancer: dynamism of the metabolism of tumor cells and tissues显示文摘 | Tsuchihara K Fujii S Esumi H | 2009 | Cancer Lett2009，278，2： | 1 |
| 14 | Comparative pharmacology of two D1-like dopamine receptors cloned from the silkworm Bombyx mori显示文摘 | Ohta H Tsuchihara K Mitsumasu K Yaginuma T Ozoe Y Asaoka K | | 0，，5： | 1 |
| 15 | Hepatitis C virus core protein enhances the activation of the transcription factor,Elk-1,in response to mitogenic stimuli显示文摘 | Fukuda K Tsuchihara K Hijikata M | 2001 | Hepatology2001，33，： | 1 |
| 16 | FOXO3a-dependent regulation of Puma in re- sponse to cytokine/growth factor withdrawal 显示文摘 | You H Pellegrini M Tsuchihara K Yamamoto K Hacker G Erlacher M | 2006 | J Exp Med2006，203，7： | 1 |
| 17 | FOXO3a-dependent regulation of Puma in response to cytokine/growth factor withdrawal 显示文摘 | You H Pellegrini M Tsuchihara K | 2006 | J Exp Med2006，203，7： | 1 |
| 18 | Copolymerization of ethylene or propylene with α-olefins containing hydroxyl groups with zirconocene/methylaluminoxane catalyst显示文摘 | Hagihara H Tsuchihara K I Takeuchi K | | 0，，： | 1 |
| 19 | Glow - discharge - inducded graft polymerization of acrylic acid onto polyfilm显示文摘 | Toshio Masuda Masaaki Kotoura Kenji Tsuchihara | 1991 | Journal of Applied Polymer Science1991，43，： | 1 |
| 20 | TAp73 knockout shows ge- nomic instability with infertility and tumor suppressor functions显示文摘 | Tomasini R Tsuchihara K Wilhelm M | 2008 | Genes Dev2008，22，19： | 1 |
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