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| 1 | Self-lubricating behaviors of Al 2 O 3 /TiB 2 ceramic tools in dry high-speed machining of hardened steel显示文摘 | Deng Jianxin Cao Tongkun Liu Lili | 2004 | Journal of the European Ceramic Society2004，，7： | 1 |
| 2 | Self-lubricating behaviors of Al2O3/TiB2 ceramic tools in dry high-speed machining of hardened steel 显示文摘 | DENG Jianxin CAO Tongkun LIU Lili | 2005 | J Eur Ceram Soc2005，25，7： | 1 |
| 3 | Self-lubricating behaviors of Al 2 O 3 /TiB 2 ceramic tools in dry high-speed machining of hardened steel显示文摘 | Deng Jianxin Cao Tongkun Liu Lili | 2004 | Journal of the European Ceramic Society2004，，7： | 1 |
| 4 | Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots显示文摘Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding. | Huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou | 2024 | Horticultural Plant Journal2024，10，2： | 0 |
| 5 | Boosting oxygen evolution reactivity by modulating electronic structure and honeycomb-like architecture in Ni_(2)P/N,P-codoped carbon hybrids显示文摘Oxygen evolution reaction(OER)as the foremost stumbling block to generate cost-effective clean fuels has received extensive attention in recent years.But,it still maintains the challenge to manipulate the geometric and electronic structure during single reaction process under the same conditions.Herein,we report a simple self-template strategy to generate honeycomb-like Ni_(2)P/N,P-C hybrids with preferred electronic architecture.Experiments coupled with theoretical results revealed that the synthesized catalyst has two characteristics:firstly,the unique honeycomb-like morphology not only enables the fully utilization of catalytic active sites but also optimizes the mass/electron transportation pathway,which favor the diffusion of electrolyte to accessible active sites.Secondly,N,P-C substrate,on the one hand,largely contributes the electronic distribution near Fermi level(E_(F))thus boosting its electrical conductivity.On the other hand,the support effect result in the upshift of d-band center and electropositivity of Ni sites,which attenuates the energy barrier for the adsorption of OH~àand the formation of*OOH.In consequence,the optimized Ni_(2)P/N,P-C catalysts feature high electrocatalytic activity towards OER(a low overpotential of 252 m V to achieve10 m A cm^(-2))and 10 h long-term stability,the outstanding performance is comparable to most of transition metal catalysts.This work gives a innovative tactics for contriving original OER electrocatalysts,inspirng deeper understanding of fabricating catalysts by combining theoretical simulation and experiment design. | Menglei Yuan Yu Sun Yong Yang Jingxian Zhang Sobia Dipazir Tongkun Zhao Shuwei Li Yongbing Xie He Zhao Zhanjun Liu Guangjin Zhang | 2021 | Green Energy & Environment2021，6，6： | 0 |
| 6 | BcRISP1,isolated from non-heading Chinese cabbage,decreases the seed set of transgenic Arabidopsis显示文摘Mitochondria are the energy sources of plant cells and are involved in regulating cell development.Ubiquinol–cytochrome c reductase iron-sulfur protein,which is necessary for mitochondrial respiration,is a subunit of mitochondrial electron transport chain multimeric enzyme complexes.To better understand the biological function of the ubiquinol–cytochrome c reductase iron–sulfur protein,the full-length cDNA of BcRISP1 was cloned;it was found to contain 810 base pairs and encode 269 amino acids.Unusually,high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction(qRT-PCR)analysis of cytoplasmic male sterile lines and maintainer lines.The seed set was affected by the overexpression of BcRISP1,and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants.These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth.qRT-PCR results revealed that in 35S::BcRISP1 plants,the expression levels of the mitochondrial respiratory chain-related genes,COX10 and RIP1,were enhanced,whereas the expression levels of QCR7 and SDH2-1 were reduced.This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore,reducing the seed set. | Tongkun Liu Yu Qian Weike Duan Jun Ren Xilin Hou Ying Li | 2014 | Horticulture Research2014，1，1： | 0 |
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