|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Improving patients'knowledge of the fetal anomaly scan显示文摘 | Smith RP Titmarsh S Overton TG | 2004 | Ultrasound Obstet Gynecol2004，24，7： | 1 |
| 2 | Terazosin:a review of its pharmacodynamic and pharmacokinetic properties,and therapeutic efficacy in essential hypertension显示文摘 | Titmarsh S Monk JP | | 0，，04： | 1 |
| 3 | How common are myeloproliferative neoplasms?A systematic review and meta-analysis显示文摘 | Titmarsh GJ Duncombe AS Mc Mullin MF | 2014 | Am J Hematol2014，89，6： | 1 |
| 4 | Generation of a human embryonic stem cell line stably expressing high levels of the fluorescent protein mCherry显示文摘AIM:The generation and characterization of a human embryonic stem cell (hESC) line stably expressing red fluorescent mCherry protein. METHODS:Lentiviral transduction of a ubiquitously-expressed human EF-1α promoter driven mCherry transgene was performed in MEL2 hESC. Red fluore-scence was assessed by immunofluorescence and flow cytometry. Pluripotency of stably transduced hESC was determined by immunofluorescent pluripotency marker expression, flow cytometry, teratoma assays andembryoid body-based differentiation followed by reverse transcriptase-polymerase chain reaction. Quantification of cell motility and survival was performed with time lapse microscopy. RESULTS:Constitutively fluorescently-labeled hESCs are useful tools for facile in vitro and in vivo tracking of survival, motility and cell spreading on various surfaces before and after differentiation. Here we describe the generation and characterization of a hESC line (MEL2) stably expressing red fluorescent protein, mCherry. This line was generated by random integration of a fluorescent protein-expressing cassette, driven by the ubiquitously-expressed human EF-1α promoter. Stably transfected MEL2-mCherry hESC were shown to express pluripo-tency markers in the nucleus (POU5F1/OCT4, NANOG and SOX2) and on the cell surface (SSEA4, TRA1-60 and TG30/CD9) and were shown to maintain a normal karyotype in long-term (for at least 35 passages) culture. MEL2-mCherry hESC further readily differentiated into representative cell types of the three germ layers in embryoid body and teratoma based assays and, importantly, maintained robust mCherry expression throughout differentiation. The cell line was next adapted to singlecell passaging, rendering it compatible with numerous bioengineering applications such as measurement of cell motility and cell spreading on various protein modified surfaces, quantification of cell attachment to nanoparticles and rapid estimation of cell survival. CONCLUSION:The MEL2-mCherry hESC line conforms to the criteria of bona fide pluripotent stem cells and maintains red fluorescence throughout differentiation, making it a useful tool for bioengineering and in vivo tracking experiments. | Dmitry A Ovchinnikov Jennifer P Turner Drew M Titmarsh Nilay Y Thakar Dong Choon Sin Justin J Cooper-White Ernst J Wolvetang | 2012 | World Journal of Stem Cells2012，4，7： | 1 |
| 5 | Terazosin:a review of pharmacodyna mic and pharmacokineticproperties, and terapeutic efficacy inessen-tialhypertension显示文摘 | Titmarsh S Monk J P | 1987 | Drugs1987，33，4： | 1 |
| 6 | No-tillage and conservation fanning practices in grain growing areas of Queensland- a review of 40 years of development显示文摘 | Thomas G A Titmarsh G W Freebaim D M | 2007 | Australian Journal of Experimental Agriculture2007，47，8： | 1 |
| 7 | Community-ac- quired infections and their association with myeloid malignancies 显示文摘 | Titmarsh GJ McMullin MF McShane CM | 2014 | Cancer Epidemiol2014，38，1： | 1 |
| 8 | Community - ac- quired infections and their association with myeloid malignancies 显示文摘 | Titmarsh GJ Mcmullin MF Mcshane CM | 2014 | Cancer Epidemiology2014，38，1： | 1 |
| 9 | Optimization of flow rate for expansion of human embryonic stem ceils in perfusion mi- crobioreactors显示文摘 | Titmarsh D Hidalgo A Turner J | 2011 | Biotechnol Bioeng2011，108，12： | 1 |
| 10 | No-tillage and conservation farming practices in grain growing areas of Queensland-A review of 40 years of development显示文摘 | Thomas G A Titmarsh G W Freebairn D M | 2007 | Australian Journal of Experimental Agriculture2007，47，8： | 1 |
| 11 | No-till- age and conservation farming practices in grain growing areas of Queensland-A review of 40 years of development 显示文摘 | Thomas GA Titmarsh GW Freebairn DM | 2007 | Australian Journal of Experimental Agriculture2007，47，8： | 1 |
| 12 | Microbio- reactor array screening of Wnt modulators and microen- vironmental factors in osteogenic differentiation ofmesen- chymal progenitor cells显示文摘 | Frith JE Titmarsh DM Padmanabhan H | 2013 | PLoS ONE2013，8，82： | 1 |
| 13 | How com- mon are myeloproliferative neoplasms? A systematic review and meta-analysis显示文摘 | Titmarsh GJ Duncombe AS McMullin MF | 2014 | Am J Hematol2014，89，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员