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| 1 | Effects of salvianolic acid B on proliferation, neurite outgrowth and differentiation of neural stem cells derived from the cerebral cortex of embryonic mice显示文摘Salvianolic acid B is isolated from Salvia miltiorrhiza,the root of which is widely used as a traditional Chinese medicine to treat stroke.However,little is known about how salvianolic acid B influences growth characteristics of neural stem cells (NSCs).The purpose of the present study was to evaluate the effects of salvianolic acid B on proliferation,neurite outgrowth and differentiation of NSCs derived from the cerebral cortex of embryonic mice using MTT,flow cytometry,immunofluorescence and RT-PCR.It was found that 20 μg mL·1 and 40 μg mL·1 salvianolic acid B had similar effects on proliferation of NSCs,and a suitable concentration of salvianolic acid B increased the number of NSCs and their derivative neurospheres.The growth-promoting activity of salvianolic acid B was dependent on and associated with an accumulation in the G2/S-phase cell population.Salvianolic acid B also promoted the neurite outgrowth of NSCs and their differentiation into neurons.The mRNA for tau,GFAP and nestin were present in differentiating neurospheres induced by salvianolic acid B.However,high-level expression of tau mRNA and low-level expression of GFAP mRNA was detected in differentiated cells,in contrast to the control conditions.This collective evidence indicates that exogenous salvianolic acid B is capable of promoting proliferation of neurospheres and differentiation towards the neuronal lineage in vitro and may act in the proliferation of NSCs and may promote NSC differentiation into neuronal cells. | GUO GuoQing1,LI Bin1,WANG YuanYuan1,SHAN AiJing1,SHEN WeiZai1,YUAN Lin2 & ZHONG ShiZhen2 1Department of Anatomy,Medical College of Jinan University,Guangzhou 510630,China 2Institute of Clinical Anatomy,Key Laboratory of Tissue Construction and Detection of Guangdong Province,Southern Medical University,Guangzhou 510150,China | 2010 | Science China(Life Sciences)2010，53，6： | 21 |
| 2 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
| 3 | Integrin betal mediates hepatocellular carcinoma cells chemotaxis to laminin显示文摘BACKGROUND: Chemotaxis is an important step durin the invasion of carcinoma cells. And integrins are most im portant receptors mediating interaction between cells an extracellular matrix ( ECM). This study was designed t study integrin betal mediating chemotaxis of hepatocellula carcinoma (HCC) cells to laminin (LN). METHODS: A micropipette technique was adopted to in vestigate the effect of blockade of integrin betal on pseudo pod protrusion of HCC cells in response to LN stimulation Chemotactic pseudopod protrusion of a HCC cell was eva luated using a dual-pipette set-up, in which two pipette filled with LN solution were positioned in close contact wit the same cell, and pseudopod protrusion into each pipett was viewed dynamically and recorded with a tape recorder The lengths of pseudopods were measured and plotte against time to obtain a pseudopod growth curve. The in tegrin betal subunit on the surfaces of HCC cells were ana lyzed by flow cytometry. RESULTS: In dual pipette chemotaxis experiment, whe the two pipettes were filled with LN(50 μg/ml, 200 μg/ml) pseudopods extended from the HCC cell into each of th pipettes nearly symmetrically, ie, with nearly identica maximum pseudopod length and similar pseudopod growth curves. Upon addition of anti-CD29 (20 μg/ml) to one o the pipettes, pseudopod protrusion was blocked nearly completely while protrusion into the opposite pipette be came more evidently, with a larger maximum length. Ex pression of integrin betal was up to 95.78% to cells chosen in the experiment. CONCLUSION: Integrin betal subunit was an importan constituent receptor subunit for mediating chemotactic pseudopod protrusion of HCC cell to LN. | Bian-Hong Fu, Ze-Zhi Wu and Cheng Dong Chongqing, China College of Bioengineering, Key Lab for Biomechanics and Tissue Engineering under the State Ministry of Education and College of Resources & Environmental Science , Chongqing University, Chongqing 400044, China Bioengineering Pro- gram, Pennsylvania State University, 205 Hallowell Building, University Park, Pennsylvania 16802-6804, USA | 2004 | Hepatobiliary & Pancreatic Diseases International2004，3，4： | 4 |
| 4 | Results of the TIMI IIIB显示文摘 | TIM/IIIB investigators effects of tissue plasrninogen activator and comparison of early Invasive and conservative st rategies in unstable angina and Non-Q-wave myocardial infraction | 1994 | Circulation1994，89，： | 1 |
| 5 | 显示文摘 | A Determination of total cationic a total anionic arsenic species in oyster tissue usi microwave-assisted extraction followed HPLC-ICP-MS | 2000 | Talanta2000，51，2： | 1 |
| 6 | Photosynthetic characteristics in canopies of Quercus rubra, Quercus prinus and Acer rubrum differ in response to soil water availability 显示文摘 | TURNBULL M H WHITEHEAD D TISSUE D T | 2002 | Oecologia2002，130，4： | 1 |
| 7 | Responses of leaf respiration to temperature and leaf characteristics in three deciduous tree species vary with site water availability 显示文摘 | TURNBULL M H WHITEHEAD D TISSUE D T | 2001 | Tree Physiology2001，21，9： | 1 |
| 8 | Soil Microbial Responses to Temporal Variations of Moisture and Temperature in a Chihuahuan Desert Grassland显示文摘 | Colin Bell Nancy McIntyre Stephen Cox David Tissue John Zak | 2008 | Microbial Ecology2008，，1： | 1 |
| 9 | Photosynthetic responses of cottonwood seedlings grown in glacial through future atmospheric vary with phosphorus supply 显示文摘 | TISSUE D T LEWIS J D | 2010 | Tree Physiology2010，30，11： | 1 |
| 10 | Dependence of fluorescence lifetimes of Y2O3:Eu3+ nanoparticles on the surrounding medium显示文摘 | Meltzer R S Feofilov S P Tissue B | | 0，，： | 1 |
| 11 | Preparation and fluorescence spectroscopy of bulk monoclinic Eu3+∶Y2O3 and comparison to Eu3+∶Y2O3,nanocrystals显示文摘 | Williams D K Bihari B Tissue B M | 1998 | J Phys Chem B1998，102，6： | 1 |
| 12 | Added value of measuring pre- and postejection velocities in ischemic myocar- dium显示文摘 | Edvardsen T Urheim S Skulstad H Quantification of left ven- tricular systolic function by tissue Doppler echocardiography | 2002 | Circulation2002，105，17： | 1 |
| 13 | The National Institute of Neurological Disorder and Stroke rt-PA Stroke Study Group显示文摘 | Tissue plasrainogen activator for acute ischemic stroke | 1995 | N Engl J Med1995，333，： | 1 |
| 14 | Falvonol content and composition of spring onion growth hydroponically or in potting soil显示文摘 | Thompson L Morris J Peffley E Green C Pare P Tissue D Jasoni R Hutson J Wehner B Kane C | 2005 | Journal of Food Composition and Analysis2005，18，： | 1 |
| 15 | Precipitation pulses and carbon fluxes in semiarid and arid ecosystems显示文摘 | Huxman T E Snyder K A Tissue D | | 0，，： | 1 |
| 16 | Photosynthesis and seed- production under water-deficit conditions in transgenic tobacco plants that over express an arabidopasis ascorbate peroxidase gene显示文摘 | Yan J Q Wang J Tissue D | 2003 | Crop Sci2003，43，4： | 1 |
| 17 | Structural parameterization and functional prediction of antigenic polypeptome sequences with biological activity through quantitative sequence-activity models (QSAM) by molecular electronegativity edge-distance vector (VMED)显示文摘Only from the primary structures of peptides, a new set of descriptors called the molecular electro-negativity edge-distance vector (VMED) was proposed and applied to describing and characterizing the molecular structures of oligopeptides and polypeptides, based on the electronegativity of each atom or electronic charge index (ECI) of atomic clusters and the bonding distance between atom-pairs. Here, the molecular structures of antigenic polypeptides were well expressed in order to propose the auto-mated technique for the computerized identification of helper T lymphocyte (Th) epitopes. Furthermore, a modified MED vector was proposed from the primary structures of polypeptides, based on the ECI and the relative bonding distance of the fundamental skeleton groups. The side-chains of each amino acid were here treated as a pseudo-atom. The developed VMED was easy to calculate and able to work. Some quantitative model was established for 28 immunogenic or antigenic polypeptides (AGPP) with 14 (1― 14) Ad and 14 other restricted activities assigned as '1'(+) and '0'(-), respectively. The latter comprised 6 Ab(15-20), 3 Ak(21-23), 2 Ek(24-26), 2 H-2k(27 and 28) restricted sequences. Good results were obtained with 90% correct classification (only 2 wrong ones for 20 training samples) and 100% correct prediction(none wrong for 8 testing samples); while con-trastively 100% correct classification (none wrong for 20 training samples) and 88% correct classification (1 wrong for 8 testing samples). Both stochastic samplings and cross valida-tions were performed to demonstrate good performance. The described method may also be suitable for estimation and prediction of classes I and II for major histocompatibility an-tigen (MHC) epitope of human. It will be useful in immune identification and recognition of pro-teins and genes and in the design and devel-opment of subunit vaccines. Several quantitative structure activity relationship (QSAR) models were developed for various oligopeptides and polypeptides including 58 dipeptides and 31 pentapeptides with angiotensin converting enzyme (ACE) inhibition by multiple linear regression (MLR) method. In order to explain the ability to characterize molecular structure of polypeptides, a molecular modeling investigation on QSAR was performed for functional prediction of polypeptide sequences with anti-genic activity and heptapeptide sequences with tachykinin activity through quantitative se-quence-activity models (QSAMs) by the molecular electronegativity edge-distance vector (VMED). The results showed that VMED exhibited both excellent structural selectivity and good activity prediction. Moreover, the results showed that VMED behaved quite well for both QSAR and QSAM of poly-and oli-gopeptides, which exhibited both good estimation ability and prediction power, equal to or better than those reported in the previous references. Finally, a preliminary conclusion was drwan: both classical and modified MED vectors were very useful structural descriptors. Some suggestions were proposed for further studies on QSAR/QSAM of proteins in various fields. | LI ZhiLiang1,2, WU ShiRong1,2, CHEN ZeCong1,2, YE Nancy1,2, YANG ShengXi1,2, LIAO ChunYang1,2, ZHANG MengJun1,2,3, YANG Li1,2, MEI Hu1,2,4, YANG Yan1,2, ZHAO Na1,2, ZHOU Yuan1,2, ZHOU Ping1,2, XIONG Qing1,2, XU Hong1,2, LIU ShuShen1,2, LING ZiHua1,2, CHEN Gang1,2,4 & LI GenRong1,2 1 College of Chemistry and Chemical Engineering/Key Laboratory for Chemobiomedical Science and Engineering under Chongqing Municipality, College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education, Chongqing University, Chongqing 400044, China 2 State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at Hunan University, Changsha 410012, China 3 Department of Medical Analysis/PLA Center of Bioinformatics Immunology, Surgeon Third University, Chongqing 400031, China 4 Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore | 2007 | Science China(Life Sciences)2007，50，5： | 1 |
| 18 | The National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group显示文摘 | Tissue plasminogen activator for acute ischemic stroke | 1995 | N Engl J Med1995，333，24： | 1 |
| 19 | The National Institute of Neurological Disorder and Stroke rt-PA Stroke Study Group显示文摘 | Tissue plasminogen activator for acute ischemic stroke | 1995 | N Engl J Med1995，333，24： | 1 |
| 20 | The National Institute of Neurological Disorders and Stroke rt- PA Stroke Study Group显示文摘 | Tissue plasminogen activator for acute isehemic stroke | 1995 | N Engl J Med1995，333，24： | 1 |
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