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| 1 | Left Atrial Function After Ablation for Paroxysmal Atrial Fibrillation显示文摘 | Ana Clara Tude Rodrigues Mauricio I. Scannavacca Márcia Azevedo Caldas Viviane Tiemi Hotta Cristiano Pisani Eduardo A. Sosa Wilson Mathias | 2009 | The American Journal of Cardiology2009，，3： | 1 |
| 2 | Nutrition claims for functional guava mousses produced with milk fat substitution by inulin and/or whey protein concentratebased on heterogeneous food legislations 显示文摘 | TIEMY lk K FLIVIA C A B ROBERTA C S | 2013 | Food Science and Technology2013，，50： | 1 |
| 3 | Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulnte wound gene express显示文摘 | Creelrnan R A Tiemy M L Mullet J E | 1992 | Proc Natl Acad Sci VSA1992，108，： | 1 |
| 4 | CRIB score, birth weight and gestational age in neonatal mortality risk evaluation显示文摘 | Angela Sara J de Britoa Tiemi Matsuob Maria Rafaela C Gonzaleza et aI | 2003 | Rev Sa0 de PO blica2003，37，5： | 1 |
| 5 | Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression 显示文摘 | Creelman R A Tiemy M L Mullet J E | 1992 | Proc Natl Acad Sci USA1992，89，： | 1 |
| 6 | Microbial conversion of n-alkanes into glycolipid biosurfactants, mannosylerythritol lipids, by Pseudozyma (Candida antarctica)显示文摘 | Dai Kitamoto Toru Ikegami Gaby Tiemi Suzuki Akira Sasaki Yu-ichiro Takeyama Yasushi Idemoto Nobuyuki Koura Hiroshi Yanagishita | 2001 | Biotechnology Letters2001，，20： | 1 |
| 7 | Antiulcer Effect of Bark Extract of Tabebuia avellanedae : Activation of Cell Proliferation in Gastric Mucosa During the Healing Process显示文摘 | Isabela Tiemy Pereira Lígia Moura Burci Luisa Mota Silva Cristiane Hatsuko Baggio Melina Heller Gustavo Amadeu Micke Moacir Geraldo Pizzolatti Maria Consuelo Andrade Marques Maria Fernanda Paula Werner | 2013 | Phytother Res2013，，7： | 1 |
| 8 | Previous Cesarean decivery and the risk of ectopic pregnancy 显示文摘 | Tiemy EF Lawson HW | 1996 | Obstet Gynecol1996，87，： | 1 |
| 9 | Topical amniotic fluid:a potential new alternative for ocular surface diseases显示文摘 | Martins SA Tiemi R | 2008 | Arq Bras Oftalmol2008，71，6： | 1 |
| 10 | The protein tyrosine phosphatase SHP-1 regulates phagolysosome bio- genesis 显示文摘 | Gomez CP Tiemi Shio M Duplay P | 2012 | J Immunol2012，189，5： | 1 |
| 11 | Profile of People Living with HIV/AIDS in a Large Municipality of Sao Paulo State, Brazil (2012-2013)显示文摘 | Gabriela Tavares Magnabosco Livia Maria Lopes Mayara Falico Faria Maria Eugenia Firmino Brunello Tiemi Arakawa Aline Araujo Antunes Rubia Laine de Paula Andrade Aline Aparecida Monroe Tereza Cristina Scatena Villa | 2014 | Journal of Health Science2014，2，2： | 0 |
| 12 | Radiographic and tomographic description of marlin sucker Remora osteochir, Pisces: Echeneidae—preliminary data of one specimen显示文摘Remoras are commensal fish of various marine species,such as sharks,swordfishes,turtles,dolphins,manta rays and whales.One specimen of marlin sucker Remora osteochir was evaluated using computed tomography for skeletal digital reconstruction,digital radiology for general evaluation and bone counting,and the double contrast technique to distinguish coelomic organs.In radiographic images,it was possible to observe otoliths in the center of the neurocranium,to count 27 vertebrae,nine pairs of ribs,and to detect the presence of the hipural and epural bones near of the caudal fin.In double contrast images,it was possible to visualize the swim bladder,gills,heart,liver,stomach and intestines.From the tomographic images and reconstructions,it was possible to identify the intercalar bones of the cephalic disc;the spine with vertebral bodies composed of neural arches and ventral ribs;and the pectoral girdle formed by posttemporal,cleithrum and scapulocoracoid bones.It was concluded that digital radiology and computed tomography were able to describe anatomical structures of marlin sucker R.osteochir without the need for dissection. | AndréLuiz Veiga Conrado Renata Stecca lunes Carlos Eduardo Malavasi Bruno Aline Tiemi Shiraishi Rocha JoséRoberto Machado Cunha da Silva | 2020 | Marine Life Science & Technology2020，2，3： | 0 |
| 13 | Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model显示文摘BACKGROUND Mesenchymal stem cells(MSCs) have been widely tested for their therapeutic efficacy in the ischemic brain and have been shown to provide several benefits. A major obstacle to the clinical translation of these therapies has been the inability to noninvasively monitor the best route, cell doses, and collateral effects while ensuring the survival and effective biological functioning of the transplanted stem cells. Technological advances in multimodal imaging have allowed in vivo monitoring of the biodistribution and viability of transplanted stem cells due to a combination of imaging technologies associated with multimodal nanoparticles(MNPs) using new labels and covers to achieve low toxicity and longtime residence in cells.AIM To evaluate the sensitivity of triple-modal imaging of stem cells labeled with MNPs and applied in a stroke model.METHODS After the isolation and immunophenotypic characterization of human bonemarrow MSCs(hBM-MSCs), our team carried out lentiviral transduction of these cells for the evaluation of bioluminescent images(BLIs) in vitro and in vivo. In addition, MNPs that were previously characterized(regarding hydrodynamic size, zeta potential, and optical properties), and were used to label these cells,analyze cell viability via the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and BLI analysis, and quantify the internalization process and iron load in different concentrations of MNPs via magnetic resonance imaging(MRI),near-infrared fluorescence(NIRF), and inductively coupled plasma-mass spectrometry(ICP-MS). In in vivo analyses, the same labeled cells were implanted in a sham group and a stroke group at different times and under different MNP concentrations(after 4 h or 6 d of cell implantation) to evaluate the sensitivity of triple-modal images.RESULTS hBM-MSC collection and isolation after immunophenotypic characterization were demonstrated to be adequate in hBM samples. After transduction of these cells with luciferase(hBM-MSCLuc), we detected a maximum BLI intensity of 2.0 x10^8 photons/s in samples of 10~6 hBM-MSCs. Analysis of the physicochemical characteristics of the MNPs showed an average hydrodynamic diameter of 38.2 ±0.5 nm, zeta potential of 29.2 ± 1.9 mV and adequate colloidal stability without agglomeration over 18 h. The signal of iron load internalization in hBM-MSCLuc showed a close relationship with the corresponding MNP-labeling concentrations based on MRI, ICP-MS and NIRF. Under the highest MNP concentration, cellular viability showed a reduction of less than 10% compared to the control.Correlation analysis of the MNP load internalized into hBM-MSCLuc determined via the MRI, ICP-MS and NIRF techniques showed the same correlation coefficient of 0.99. Evaluation of the BLI, NIRF, and MRI signals in vivo and ex vivo after labeled hBM-MSCLuc were implanted into animals showed differences between different MNP concentrations and signals associated with different techniques(MRI and NIRF; 5 and 20 μg Fe/mL; P < 0.05) in the sham groups at 4 h as well as a time effect(4 h and 6 d; P < 0.001) and differences between the sham and stroke groups in all images signals(P < 0.001).CONCLUSION This study highlighted the importance of quantifying MNPs internalized into cells and the efficacy of signal detection under the triple-image modality in a stroke model. | Helio Rodrigues da Silva Javier Bustamante Mamani Mariana Penteado Nucci Leopoldo Penteado Nucci Andrea Tiemi Kondo Daianne Maciely Carvalho Fantacini Lucas Eduardo Botelho de Souza Virginia Picanço-Castro Dimas Tadeu Covas José Mauro Kutner Fernando Anselmo de Oliveira Nelson Hamerschlak Lionel Fernel Gamarra | 2019 | World Journal of Stem Cells2019，11，2： | 0 |
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