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157篇 您的检索式：作者名="Tetlow"

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	题名	作者	年代	出处	被引量
1	小麦胚乳14-3-3蛋白的表达及其与淀粉体淀粉合成酶的互作显示文摘从小麦胚乳中克隆了14-3-3基因,并将其分别插入pET29c和pET41c质粒,用热激法转化大肠杆菌BL21-CodonPlus(DE3)-RP,得到高效表达的蛋白,但融合蛋白主要以包涵体的形式存在。可溶性的融合蛋白可直接通过S-蛋白琼脂糖树脂纯化。包涵体经8molL-1尿素溶解变性,稀释复性后,结合到S-蛋白琼脂糖树脂上,也得到纯化的融合蛋白。复性后的融合蛋白对蔗糖合酶活性表现抑制作用,说明包涵体14-3-3融合蛋白恢复活性。将结合14-3-3融合蛋白的S-蛋白琼脂糖树脂作为诱饵与小麦胚乳淀粉体提取液进行亲和杂交,与14-3-3蛋白特异互作的淀粉合成酶结合到S-蛋白琼脂糖树脂上,Western检测结果表明,淀粉体淀粉合酶I(SSI)、淀粉合酶II(SSII)、淀粉分支酶Iia(SBEIIa)、淀粉分支酶Iib(SBEIIb)和ADP焦磷酸化酶大亚基(SH2)与14-3-3蛋白存在互作,而淀粉分支酶I(SBEI)、淀粉磷酸化酶(SP)、D-酶(DE)和ADP焦磷酸化酶小亚基(BT2)不能与14-3-3蛋白结合,说明小麦胚乳14-3-3蛋白对淀粉体淀粉合成具有一定的调控作用。	宋健民戴双李豪圣刘爱峰程敦公楚秀生 Ian J TETLOW Michael J EMES	2009	作物学报2009，35，8：	5
2	Subcellular localization of ADPglucose pyrophosphorylase in developing wheat endosperm and analysis of the properties of a plastidial isoform显示文摘	Tetlow I J Davies E J Vardy K A		0，，：	1
3	Cottonseed Extraction with Mixtures of Acetone and Hexane显示文摘	M S Kuk R Tetlow M K Dowd	2005	Journal of the American Oil Chemists'' Society2005，82，8：	1
4	The effect of urea on the preservation and di- gestibilityin vitro of perennial ryegrass 显示文摘	Tetlow R M	1983	Anim Feed Sci Technol1983，，10：	1
5	Matrix metalloproteinase and proin-flammatory cytokine production by chondrocytcs of human osteoarthriticcartilage: associations with degenerative changes显示文摘	Tetlow LC Adlam DJ Woolley DE	2012	Arthritis Rheum2012，44，3：	1
6	Subcellular localization of ADP-glucose pyrophosphorylase in developing wheat endosperm and analysis of the properties of a plastidial isoform 显示文摘	Tetlow I J Davies E J Vardy K A	2003	J Exp Bot2003，54，：	1
7	Matrix metalloproteinase and proinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage: associations with degenerative changes 显示文摘	Tetlow L C Adlam D J Woolley D E	2001	Arthritis & Rheumatism2001，44，3：	1
8	Characterization of PAN- based carbon fibers with laser Raman spectroscopy 显示文摘	Melanitis N Tetlow P L Galiotis C	1996	J Mater Sci1996，31，4：	1
9	Prepared for Retirement? The Adequacy and Distribution of Retirement Re- sources in England 显示文摘	Banks J C EmmersonZOldfield G Tetlow	2005	London:The Institute for Fiscal Stud- ies2005，，：	1
10	Expression of vitamin D receptors and matrix metalloproteinases in osteoarthritic cartilage and human articular chondrocytes in vitro显示文摘	Tetlow LC Woolley DE	2001	Osteoarthritis Cartilage2001，9，：	1
11	Characterization of the human Omega class glutathione transferase genes and associated polymorphisms 显示文摘	Whitbread AK Tetlow N Eyre HJ	2003	Pharmaeogenetics2003，13，3：	1
12	查看详情显示文摘	Woolley D E Tetlow L C Adlam D J		0，，：	1
13	ATP8B 1 mutationsin British cases with intrahepatic cholestasis of pregnancy 显示文摘	R MULLENBAGH A BENNETT N TETLOW	2005	Gut2005，54，6：	1
14	Characterization of the human omega class glutathione transferase genes and associated polymorphisms 显示文摘	Whitbread AK Tetlow N Eyre H J	2003	Pharmacogenetics2003，13，3：	1
15	Characterization of PAN-based carbon fibers with laser Raman spectroscopy 显示文摘	N Melanitis PL Tetlow C Galiotis	1996	J Mater Sci1996，31，4：	1
16	Prepared for Retirement? 显示文摘	Banks J C Emmerson Z Oldfield G Tetlow	2005	The Adequacy and Distribution of Retirement Re-sources in England2005，，：	1
17	Histological analysis of the uterine junctional zone as seen by transvaginal ultrasound显示文摘	Tetlow R Richmond I Manton D	1999	Ultrasound Obstet Gynecol1999，14，3：	1
18	Recent developments in understanding the regulation of starch metabolism in higher plants 显示文摘	Tetlow I J Morell M K Emes M J	2004	J Exp Bot2004，55，406：	1
19	Matrix metalloproteinase and roinflammatory cytokine production by chondrocytes of human osteoarthritic cartilage显示文摘	Tetlow LC Adlam DJ Woolley DE	2001	Arthritis Rheum2001，44，：	1
20	Ultrasound evaluation of the uterine zonal anatomy during in-vitro fertilization and embryo transfer显示文摘	Lesny P Killick SR Tetlow RL	1999	Hum Reprod1999，14，6：	1

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