|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system显示文摘Background:Amyloid beta peptide(Aβ)is the main component of extraneuronal senile plaques typical of Alzheimer’s disease(AD)brains.Although Aβis produced by normal neurons,it is shown to accumulate in large amounts within neuronal lysosomes in AD.We have recently shown that under normal conditions the majority of Aβis localized extralysosomally,while oxidative stress significantly increases intralysosomal Aβcontent through activation of macroautophagy.It is also suggested that impaired Aβsecretion and resulting intraneuronal increase of Aβcan contribute to AD pathology.However,it is not clear how Aβis distributed inside normal neurons,and how this distribution is effected when Aβsecretion is inhibited.Methods:Using retinoic acid differentiated neuroblastoma cells and neonatal rat cortical neurons,we studied intracellular distribution of Aβby double immunofluorescence microscopy for Aβ40 or Aβ42 and different organelle markers.In addition,we analysed the effect of tetanus toxin-induced exocytosis inhibition on the intracellular distribution of Aβ.Results:Under normal conditions,Aβwas found in the small cytoplasmic granules in both neurites and perikarya.Only minor portion of Aβwas colocalized with trans-Golgi network,Golgi-derived vesicles,early and late endosomes,lysosomes,and synaptic vesicles,while the majority of Aβgranules were not colocalized with any of these structures.Furthermore,treatment of cells with tetanus toxin significantly increased the amount of intracellular Aβin both perikarya and neurites.Finally,we found that tetanus toxin increased the levels of intralysosomal Aβalthough the majority of Aβstill remained extralysosomally.Conclusion:Our results indicate that most Aβis not localized to Golgi-related structures,endosomes,lysosomes secretory vesicles or other organelles,while the suppression of Aβsecretion increases intracellular intra-and extralysosomal Aβ. | Lin Zheng Angel Cedazo-Minguez Martin Hallbeck Fredrik Jerhammar Jan Marcusson Alexei Terman | 2012 | Translational Neurodegeneration2012，1，1： | 10 |
| 2 | Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor显示文摘 | Terman BI Dougher Vermazen M | 1992 | Biochem Biophys Res Commun1992，187，： | 2 |
| 3 | A controlied trial of timed bright light and negative air ionization for treatment of winter de pression显示文摘 | Terman M Terman J S Ross D C | 1998 | Archives of General Psychiatry1998，55，10： | 1 |
| 4 | Autophagy in cardiac myocyte homeostasis, aging, and pathology 显示文摘 | Terman A Brunk UT | 2005 | Cardiovasc Res2005，68，3： | 1 |
| 5 | Global competition and local cooperation in a network of neural oscillator显示文摘 | TERMAN D WANG Deliang | 1995 | Physica D: Nonlinear Phenomena1995，81，12： | 1 |
| 6 | The conservation of talent显示文摘 | TERMAN LM | 1924 | School and Society1924，，19： | 1 |
| 7 | Incidence of and risk factors for superior facet violation in minimally invasive versus open pedicle screw placement during transforami- nal lumbar interbody fusion: a comparative analysis显示文摘 | Lau D Terman SW Patel R | 2013 | J Neurosurg Spine2013，18，4： | 1 |
| 8 | Randomized clin-ical trial of bright light therapy for antepartum depression : pre-liminary findings 显示文摘 | Epperson CN Terman M Terman JS | 2004 | J Clin Psychiatry2004，65，3： | 1 |
| 9 | Autophagy,organelles and ageing显示文摘 | Terman A Gustafsson B Brunk UT | 2007 | J Pathol2007，211，： | 1 |
| 10 | Extracorporeal immunoad- sorption: initial experience in human systemic lupus erythematosus 显示文摘 | Terman DS Buffaloe G Mattio|i C | 1979 | Lancet1979，2，8147： | 1 |
| 11 | Lipofuscin: mechanisms of age-relatedaccumulation and influence on cell function显示文摘 | Brunk UT Terman A | 2002 | Free Radic Biol Med2002，33，5： | 1 |
| 12 | Cytokine therpecctics: Lesson from erf erons显示文摘 | terman J U | 1994 | Proc Natl Acad S ci USA1994，，： | 1 |
| 13 | Effect of the polymorphism of prolactin receptor(PRLR)and leptin(LEP)genes on litter size in Polish pigs显示文摘 | Terman A | 2005 | J Anim Breed Genet2005，122，： | 1 |
| 14 | The semaphorins显示文摘 | Yazdani U Terman J R | 2006 | Genome Biol2006，7，21： | 1 |
| 15 | Cooperativity of staphylococcal aureus enterotoxin B superantigen, major histocompatibility complex class Ⅱ, and CD80 for immunotherapy of advanced spontaneous metastases in a clinically relevant postoperative mouse breast model 显示文摘 | Pulaski BA Terman DS KhanS | 2000 | Cancer Res2000，60，10： | 1 |
| 16 | Lysosomes and oxidative stress in aging and apoptosis 显示文摘 | Tino Kurz Alexei Terman Bertil Gustsfsson | 2008 | Biochimicaet Biophysica Acta2008，1780，： | 1 |
| 17 | Intrapleural staphylococcal superantigen induces resolution of malignant pleural effusions and a survival benefit in non-small cell lung cancer显示文摘 | Ren S Terman DS Bohanch G | 2004 | Chest2004，126，5： | 1 |
| 18 | Identification of the KDR tyrosine as a receptor for vascular endothelial growth factor显示文摘 | Terman BI Dougher VM Carrion ME | 1992 | Biochem Biophys Res Commun1992，187，3： | 1 |
| 19 | Identification of the KDR tyrosine kinase as a receptor for vascular endothelial cell growth factor显示文摘 | Terman BI Dougher-Vermazen M Carrion ME | 1992 | Biochem Bioph ys Res Commun1992，187，3： | 1 |
| 20 | Identifica- tion of the KDR tyrosine kinase as a receptor for vascular endo- thelial cell growth factor 显示文摘 | Terman BI Dougher-Vermazen M Carrion ME | 1992 | Biochem Biophys Res Commun1992，187，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员