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| 1 | Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen显示文摘Cryopreservation 与反应的氧种类的生产被联系,它导致精子膜并且因而的类脂化合物 peroxidation 在精子活动性和减少的富饶潜力的减小。这研究的目的是决定为雄野猪精液的 cryopreservation 需要的 L 半胱氨酸的最佳的集中。12 只雄野猪为这研究提供了证明活动性和形态学的精液。精液被划分成乳糖鸡蛋蛋黄( LEY )扩展程序在使用了到的四部分重新推迟 centrifuged 精子小团与L半胱氨酸的各种各样的集中被补充到达 0 mmol L ? 1 (组我,控制), 5 mmol L ? 1 (组 II ), 10 mmol L ? 1 (组 III )并且 15 mmol L ? 1 (组 IV )。精液暂停在稻草(0.5 mL ) 被装载并且在一个控制率冰箱放了。在 cryopreservation 以后,冻结精液样品为进步活动性,生存能力使用 SYBR-14/EthD-1 用染色的 FITC-PNA/EthD-1 染色和 acrosome 正直被融化并且调查。有一显著地更高(P < 0.01 ) 在与控制相比的二个 L-cysteine-supplemented 组(组 II 和组 III ) 的进步活动性,生存能力和 acrosomal 正直的百分比。有 L 半胱氨酸的 biphasic 效果,与在组 III 的进步活动性,生存能力和 acrosomal 正直的最高的百分比。在结论, 5 或 10 mmol L ? 1 是为改进冻结的鈥搕h awed 雄野猪精液的质量要加到 LEY 扩展程序的 L 半胱氨酸的最佳集中。 | Kampon Kaeoket Panida Chanapiwat Padet Tummaruk Mongkol Techakumphu | 2010 | Asian Journal of Andrology2010，12，5： | 4 |
| 2 | Characterization and in vitro culture of putative spermatogonial stem cells derived from feline testicular tissue显示文摘 | Tiptanavattana N Thongkittidilok C Techakumphu M | 2013 | J of Repord Dev2013，59，： | 1 |
| 3 | Seasonal influences on the litter size at birth of pigs are more pro- nounced in the gilt than sow litters 显示文摘 | Tummaruk P Tantasuparuk W Techakumphu M et d | 2010 | Joumal of Agricultural Science2010，148，4： | 1 |
| 4 | Transvaginal follicle aspiration in thai swamp buffalo heifers using different vaccume pressure after FSH pretreamaent (Bubalus bubalis) 显示文摘 | Techakumphu M Promidireg A Phulikanit N | 1968 | J Vet Med Sci1968，，8： | 1 |
| 5 | Ovarian responses and oocyte recovery in prepubertal swamp buffalo (bubalus bubalis)calves after FSH or PMSG treatment显示文摘 | Techakumphu M Lohachit C Tantasuparak W | 1996 | Theriogenology1996，45，： | 1 |
| 6 | Age, body weight and backfat thickness at first observed oestrus in crossbred Landrace x Yorkshire gilts, seasonal varia- tions and their influence on their subsequent reproductive per- formance 显示文摘 | TUMMARUK P TANTASUPARUK W TECHAKUMPHU M | 2007 | Animal Reproduction Science2007，99，12： | 1 |
| 7 | Influence of repeat-service and weaning-to-first-service interval on farrowing proportion of gilts and sows 显示文摘 | Tummaruk R Tantasuparuk W Techakumphu M | 2010 | Preventive Veterinary Medicine2010，96，： | 1 |
| 8 | Comparison of artificial insemination with natural mating on smallholder farms in Thailand, and the effects of boar stimulation and distance of semen delivery on sow reproductive performance 显示文摘 | Amin N Tantasuparuk W Techakumphu M | 2010 | Trop Anim Health Pro2010，42，： | 1 |
| 9 | Distribution of spermatozoa and embryos in the female reproductive tract after unilateral deep intrauterine insemination in the pig显示文摘 | Tummaruk P Sumransap P Techakumphu M et a/ | 2007 | Reprod Domest Anim2007，42，： | 1 |
| 10 | The effect of antioxidants on motility,viability,acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa显示文摘 | Tbuwanut P Chatdarong K Techakumphu M | 2008 | Theriogenology2008，70，2： | 1 |
| 11 | Characterization and in vitro culture of putative spermatogonial stem cells derived from feline testicular tissue显示文摘 | Tiptanavattana N Thongkittidilok C Techakumphu M | | 0，，02： | 1 |
| 12 | Age,body weight and backfat thickness at first observed oestrus in crossbred Landrace×Yorkshire gilts,seasonal variations and their influence on subsequence reproductive performance显示文摘 | TUMMARUK P TANTASUPARUK W TECHAKUMPHU M | 2007 | Animal Reproduction Science2007，99，12： | 1 |
| 13 | Comparison of ar- tificial insemination with natural mating on smallholder farms in Thailand, and the effects of boar stimulation and distance of semen delivery on sow reproductive performance 显示文摘 | Amin N Tantasuparuk W Techakumphu M | 2010 | Trop Anim Health Pro2010，42，： | 1 |
| 14 | Characterization and in vitro culture of putative spermatogonial stem cells derived from feline testicular tissue显示文摘 | Tiptanavattana N Thongkittidilok C Techakumphu M | | 0，，02： | 1 |
| 15 | Ovarian response and oocyte recovery in prepubertal swamp buffalo(Bubalus bubalis)calves after FSH or PMSG treatment显示文摘 | Techakumphu M C Lohachit W Tantasuparak W | 1995 | Theriogenology1995，44，： | 1 |
| 16 | Improvement of normal fertilization rate and embryo development by reduction of sperm:oocyte ratio during in vitro fertilization in pig显示文摘 | Panasophonkul S Chanapiwat T T P Techakumphu M | 2011 | Thai J Vet Med2011，41，1： | 1 |
| 17 | Repeated oocyte pickup in prepubertal swamp buffalo (Bubalus Bubalis) calves after FSH superstimulation 显示文摘 | Techakumphu M Promdireg A Nachiengmai A | 2004 | Theriogenology2004，61，： | 1 |
| 18 | Repeated oocyte pickup in prepubertal swamp buffalo (Bubalus Bubalis) ealves after FSH superstimulation显示文摘 | Techakumphu M Promdireg A | 2004 | Theriogenology2004，61，： | 1 |
| 19 | Embryoid body formation from embryonic and induced pluripotent stem cells:Benefits of bioreactors显示文摘Embryonic stem(ES)cells have the ability to differ-entiate into all germ layers,holding great promise not only for a model of early embryonic development but also for a robust cell source for cell-replacement therapies and for drug screening.Embryoid body (EB)formation from ES cells is a common method for producing different cell lineages for further applications. However,conventional techniques such as hanging drop or static suspension culture are either inherently incapable of large scale production or exhibit limited control over cell aggregation during EB formation and subsequent EB aggregation.For standardized mass EB production,a well defined scale-up platform is necessary.Recently,novel scenario methods of EB formation in hydrodynamic conditions created by bioreactor culture systems using stirred suspension systems(spinner flasks),rotating cell culture system and rotary orbital culture have allowed large-scale EB formation.Their use allows for continuous monitoring and control of the physical and chemical environment which is difficult to achieve by traditional methods.This review summarizes the current state of production of EBs derived from pluripotent cells in various culture systems.Furthermore,an overview of high quality EB formation strategies coupled with systems for in vitro differentiation into various cell types to be applied in cell replacement therapy is provided in this review. Recently,new insights in induced pluripotent stem(iPS) cell technology showed that differentiation and lineage commitment are not irreversible processes and this has opened new avenues in stem cell research.These cells are equivalent to ES cells in terms of both self-renewal and differentiation capacity.Hence,culture systems for expansion and differentiation of iPS cells can also apply methodologies developed with ES cells,although direct evidence of their use for iPS cells is still limited. | Sasitorn Rungarunlert Mongkol Techakumphu Melinda K Pirity Andras Dinnyes | 2009 | World Journal of Stem Cells2009，1，1： | 1 |
| 20 | Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility显示文摘 | Am-In N Kirkwood R N Techakumphu M | 2011 | Theriogenology2011，75，5： | 1 |
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