| 1 | Evaluation of collapse resistance of RC frame structures for Chinese schools in seismic design categories B and C显示文摘According to the Code for Seismic Design of Buildings (GB50011-2001), ten typical reinforced concrete (RC) frame structures, used as school classroom buildings, are designed with different seismic fortification intensities (SFIs) (SFI=6 to 8.5) and different seismic design categories (SDCs) (SDC=B and C). The collapse resistance of the frames with SDC=B and C in terms of collapse fragility curves are quantitatively evaluated and compared via incremental dynamic analysis (IDA). The results show that the collapse resistance of structures should be evaluated based on both the absolute seismic resistance and the corresponding design seismic intensity. For the frames with SFI from 6 to 7.5, because they have relatively low absolute seismic resistance, their collapse resistance is insufficient even when their corresponding SDCs are upgraded from B to C. Thus, further measures are needed to enhance these structures, and some suggestions are proposed. | Tang Baoxin Lu Xinzheng Ye Lieping Shi Wei | 2011 | Earthquake Engineering and Engineering Vibration2011，10，3： | 8 |
| 2 | Cloning and Functional Analysis of FLJ20420:A Novel BAG-1 Promoter Transcription Factor显示文摘BAG-1 is an anti-apoptotic protein that interacts with a variety of cellular molecules to inhibit apoptosis. It is also important in the prognosis of several human malignancies such as breast cancer. We have previously cloned the human BAG-1 promoter. Using BAG-1 promoter as a probe, we identi ed a cDNA clone that encodes a novel BAG-1 promoter-binding protein, termed FLJ20420. The FLJ20420 protein speci cally bind to BAG-1 promoter (-353 to -128 bp) and decreased BAG-1 promoter activity ~30% by cotransfecting the pcDNA3.1-FLJ20420 and BGP-Luc BAG-1 promoter into lung cancer cell lines A549 and L9981. The FLJ20420 gene encodes a ~26kD protein localized in both cytoplasm and nucleus. Comparison of human FLJ20420 with mouse sequences showed a 90% homology with mouse gene, named CHCHD3. Furthermore, Northern blot revealed that FLJ20420 has low-level transcriptional expression in most human normal tissues (brain, placenta, lung, liver, kidney, pancreas and cervix) except in heart and skeletal muscle, and has a clear expression in most tumor cell lines. Knockdown of endogenous FLJ20420 significantly increased the expression of BAG-1 in A549 and L9981 cells, as analyzed by real-time PCR and western blotting. Gene array studies in lung cancer tissue samples indicated that there is a significant change in expression of FLJ20420 between the primary lung cancer and the paired normal lung tissues (P=0.0002), while BAG-1 has a significantly decreased expression in the primary lung cancers compared to the paired normal lung tissues (P=0.0001) , suggesting that the expression of BAG-1 was controlled by positive as well as negative transcription factors. Our observation was further confirmedby real-time PCR analysis of FLJ20420 and BAG-1 expressions in these tissues. Taken together, our results suggest that FLJ20420 functions as a down-regulator of BAG-1 and its expression may be involved in oncogenesis of human malignancy. | Jun CHEN, Hongyu LIU, Yun BAI, Jin WANG, Heng WU, Baoxin LIU, Ying LI, Lingling ZU, Shou-Ching TANG, Qinghua ZHOU Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenviroment Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Anshan Road No.154, Heping District, Tianjin 300052, China | 2009 | 中国肺癌杂志2009，12，6： | 0 |
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