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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Internal carotid artery flow volume measurement and other intraoperative duplex scanning parameters as predictors of stroke after carotid endarterectomy显示文摘 | Ascher E Markevich N Hingorani AP e tal | 2002 | J VascSurg2002，35，3： | 1 |
| 3 | Novel activating muta- tions lacking eysteine in type I eytokinc receptors in acute lym- phoblastic leukemia显示文摘 | Shochat C Tal N Gryshkova V | 2014 | Blood2014，124，1： | 1 |
| 4 | Heterotopic pregnancy after ovulation induction and assisted reproductive technologies: a literature reviewfrom 1971 to 1993 显示文摘 | Tal J Haddad S Gordon N | 1996 | Fertility and Sterility1996，66，1： | 1 |
| 5 | Vegetative compatibility groups in Colletotrichum coccodes,the causal agent of black dot on potato显示文摘 | NITZAN N HAZANOVSKY M TAL M | 2002 | Phytopathology2002，92，8： | 1 |
| 6 | Heterotopic pregnancy after ovulation induction and assisted reproductive technologies:a literature review from 1971-1993显示文摘 | Tal J Heddad S Gordon N | 1996 | Fertil Steril1996，66，： | 1 |
| 7 | Heterotopic pregnancy after ovulation and assisted reproductive technologies:a literature review from 1971~1993显示文摘 | Haddad S Gorodon N | 1996 | Fertil Steril1996，66，1： | 1 |
| 8 | Effects of 2-month ozone expo snre in spinach leaves on photosynthesis,antioxidant systems and lipid peroxidation显示文摘 | Calatayud A Iglesias D J Talón M | 2003 | Plant Physiology and Biochemistry2003，41，9： | 1 |
| 9 | Aplasia ofthe dorsal pancreas and choledochal cyst显示文摘 | Oyachi N Ohhama Y Tal a H | 2006 | Pediatr Surg Int2006，22，6： | 1 |
| 10 | Vegetative compatibility groups in Colletotrichum coccodes, the causal agent of black dot on potato显示文摘 | Nitzan N Hazanovsky M Tal M | 2002 | Phytopathology2002，92，： | 1 |
| 11 | Mean field variational approximation for continuous-time bayes- ian networks 显示文摘 | Cohn I El-Hay Tal Friedman N Kupferman R | 2010 | Journal of Machine Learning Research2010，11，10： | 1 |
| 12 | Macroparticles TiN Films Prepared by the Arc Ion Plating Process显示文摘 | KoH E S | 1990 | Surface and Coatings Technology1990，4344，： | 1 |
| 13 | Validation of the freezing of gaitquestionnaire in patients with Parkinson's disease显示文摘 | Giladi N Tal J Azulay T | 2009 | Mov Disord2009，24，5： | 1 |
| 14 | Research on a Metal Mobility Test Device 显示文摘 | Z h o u Z h i m i n g S o n g X i a o f a n g C h e n Y u a n f a n g e tal | 2014 | Experimental Science and Technology2014，，5： | 1 |
| 15 | Laparoscopic surgery for subfertility associated with en- dometriosis 显示文摘 | Tal Z Jacobson James M N Duf fy David Barlow | 2011 | Human Reproduction Update2011，17，2： | 1 |
| 16 | Heterotopic pregnancy after ovulation and assisted reproductive technologies:a literature review from 1971-1993显示文摘 | Tal J Haddad S Gorodon N | 1996 | Fertil Steril1996，66，1： | 1 |
| 17 | Human gastrin:a helicobacter pylori-specific growthfactor显示文摘 | Chowers MY Keller N Tal R | 1999 | Gastroenterology1999，117，5： | 1 |
| 18 | Vegetative compatibility groups in Colletotrichum coccodes,the causal agent of black dot on potato显示文摘 | Nitzan N Hazanovsky M Tal M | 2002 | Phytopathology2002，92，： | 1 |
| 19 | Hydrolysis of haloacetonitriles: linear free energy relationship, ki- netics and products显示文摘 | Glezer V Harris B Tal N | 1999 | Water Research1999，33，8： | 1 |
| 20 | Gain-of-function mutations in interleukin-7 receptor-a (IL7R) in childhood acute lymphoblas- tic leukemias显示文摘 | Shochat C Tal N Bandapalli OR | 2011 | J Exp Med2011，208，5： | 1 |
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