| 1 | Effect of vitamin E and human placenta cysteine peptidase inhibitor on expression of cathepsins B and L in implanted hepatoma Morris 5123 tumor model in Wistar rats显示文摘AIM: To examine the effectiveness of human placental inhibitors, by injecting vitamin E to rats with transplanted Norris-5123 hepatoma, on the expression of cathepsins B and L in tumor, liver, lung and blood sera after transplantation of Norris 5123 hepatoma.METHODS: Animals were divided into 10 groups receiving three different concentrations of vitamin E and inhibitors along or in combination and compared with negative control (healthy rats) and positive control (tumor rats). Effectiveness of treatment was evaluated with regard to survival time,tumor response and determination of the activities of proteolytic enzymes and their inhibitors using flurogenic substrates.RESULTS: Cathepsins B and L activities were elevated by 16-fold in comparison with negative control tissues, and their endogenous inhibitor activity decreased by 1.2-fold before treatment. In several cases, tumors completely disappeared following vitamin E plus human placental cyteine protease inhibitor (CPI) compared with controls.The number of complete tumor responses was higher when 20 m/kg vitamin E plus 400 μg of CPI was used, i.e.7/10 rats survived more than two mo. Cathepsins B and L were expressed significantly in tumor, liver, lung tissues and sera in parallel to the increasing of the endogenous inhibitor activity compared with the controls after treatment (P<0.0001).CONCLUSION: The data indicate formation of metastasis significantly reduced in treated rats, which might provide a therapeutic basis for anti-cancer therapy. | Tadeusz Sebzda Piotr Hanczyc Yousif Saleh Bernice F Akinpelumi Maciej Siewinski Jerzy Rudnicki | 2005 | World Journal of Gastroenterology2005，11，4： | 2 |
| 2 | Diagnostic usefulness of selected proteases and acute phase factors in patients with colorectal adenocarcinoma显示文摘BACKGROUND Uncontrolled growth and loss of control over basic metabolic functions,leading to invasive proliferation and metastases,are the salient traits of malignant tumors in general and colorectal cancer in particular.Invasion and metastases hinder effective tumor treatment.While surgical techniques and radiotherapy can be used to remove tumor focus,only chemotherapy can eliminate dispersed neoplastic cells.However,the efficacy of the latter method is limited in the advanced stages of the disease.Therefore,recognition of the mechanisms involved in neoplastic cell spreading is indispensable for developing effective therapies.AIM To use a number of biomarkers involved in cancer progression and identify a panel that could be used for effective early diagnosis.METHODS We recruited 185 patients with colorectal adenocarcinoma(98 men,87 women with median age 63).Thirty-five healthy controls were sex and age-matched.Dukes’staging was as follows:A=22,B=52,C=72,D=39.We analyzed patients'blood serum before surgery.We determined:(1)Cathepsin B(CB)with Barrett's method(fluorogenic substrate);(2)Leukocytic elastase(LE)in a complex with alpha 1 trypsin inhibitor(AAT)using the immunoenzymatic MERCK test;(3)Total sialic acid(TSA)with the colorimetric periodate-resorcinol method;(4)Lipid-bound sialic acid(LASA)with the colorimetric Taut's method;and(5)The antitrypsin activity(ATA)employing the colorimetric test.RESULTS In patients,the values of the five biochemical parameters were as follows:CB=16.1±8.8 mU/L,LE=875±598μg/L,TSA=99±31 mg%,LASA=0.68±0.33 mg%,and ATA=3211±1504 U/mL.Except for LASA,they were significantly greater than those of controls:CB=11.4±6.5 mU/L,LE=379±187μg/L,TSA=71.4±15.1 mg%,LASA=0.69±0.28 mg%,and ATA=2016±690 U/mL.For CB and LASA,the differences between the four Dukes’stages and controls were not statistically significant.The inter-stage differences for CB and LASA were also absent.The receiver operating characteristic(ROC)analysis revealed the potential diagnostic value of CB,TSA,and ATA.The area under ROC,sensitivity,and specificity for these three parameters were:0.85,72%,90%;0.75,66%,77%;and 0.77,63%,84%,respectively.The sensitivity and specificity for the threeparameter panel CB-TSA-ATA were equal to 88.2%and 100%,respectively.CONCLUSION The increased value of CB,TSA,and ATA parameters are associated with tumor biology,invasion,and metastasis of colorectal cancer.The presented evidence suggests the potential value of the CB-TSA-ATA biochemical marker panel in early diagnostics. | Tadeusz Sebzda Jan Gnus Barbara Dziadkowiec Miroslaw Latka Jakub Gburek | 2021 | World Journal of Gastroenterology2021，27，39： | 0 |
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