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1篇 您的检索式：作者名="TSENG Chengkui"

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		题名	作者	年代	出处	被引量
	1	Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales,Phaeophyta)显示文摘A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro-particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529μg/mg soluble proteins on average and the highest value was 2.497μg/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bio-reactor producing high value materials such as edible HBY vaccine was discussed as well.	JIANG Peng QIN Song TSENG Chengkui	2002	Chinese Science Bulletin2002，47，17：	14

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