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| 1 | A novel animal model for in vivo study of liver cancer metastasis显示文摘AIM:To establish an animal model with human hepatocyte-repopulated liver for the study of liver cancer metastasis.METHODS:Cell transplantation into mouse livers was conducted using alpha-fetoprotein(AFP)-producing hu-man gastric cancer cells(h-GCCs) and h-hepatocytes as donor cells in a transgenic mouse line expressing urokinase-type plasminogen activator(uPA) driven by the albumin enhancer/promoter crossed with a severe combined immunodeficient(SCID) mouse line(uPA/SCID mice).Host mice were divided into two groups(A and B).Group A mice were transplanted with h-GCCs alone,and group B mice were transplanted with h-GCCs and h-hepatocytes together.The replacement index(RI),which is the ratio of transplanted h-GCCs and h-hepatocytes that occupy the examined area of a histological section,was estimated by measuring h-AFP and h-albumin concentrations in sera,respectively,as well as by immunohistochemical analyses of h-AFP and human cytokeratin 18 in histological sections.RESULTS:The h-GCCs successfully engrafted,repopulated,and colonized the livers of mice in group A(RI = 22.0% ± 2.6%).These mice had moderately differentiated adenocarcinomatous lesions with disrupted glandular structures,which is a characteristics feature of gastric cancers.The serum h-AFP level reached 211.0 ± 142.2 g/mL(range,7.1-324.2 g/mL).In group B mice,the h-GCCs and h-hepatocytes independently engrafted,repopulated the host liver,and developed colonies(RI = 12.0% ± 6.8% and 66.0% ± 12.3%,respectively).h-GCC colonies also showed typical adenocarcinomatous glandular structures around the h-hepatocyte-colonies.These mice survived for the full 56 day-study and did not exhibit any metastasis of h-GCCs in the extrahepatic regions during the observational period.The mice with an h-hepatocyte-repopulated liver possessed metastasized h-GCCs and therefore could be a useful humanized liver animal model for studying liver cancer metastasis in vivo.CONCLUSION:A novel animal model of human liver cancer metastasis was established using the uPA/SCID mouse line.This model could be useful for in vivo testing of anti-cancer drugs and for studying the mechanisms of human liver cancer metastasis. | Shinsuke Fujiwara Hikaru Fujioka Chise Tateno Ken Taniguchi Masahiro Ito Hiroshi Ohishi Rie Utoh Hiromi Ishibashi Takashi Kanematsu Katsutoshi Yoshizato | 2012 | World Journal of Gastroenterology2012，18，29： | 6 |
| 2 | Plasma levodopa peak delay and impaired gastric emptying in Parkinson’s disease显示文摘 | Hirokazu Doi Ryuji Sakakibara Mitsutoshi Sato Tohru Masaka Masahiko Kishi Akihiko Tateno Fuyuki Tateno Yohei Tsuyusaki Osamu Takahashi | 2012 | Journal of the Neurological Sciences . 2012 (1-2)2012，，1： | 2 |
| 3 | Surgery for Hepatocellular Carcinoma Located in the Caudate Lobe显示文摘 | Masahiko Sakoda Shinichi Ueno Fumitake Kubo Kiyokazu Hiwatashi Taro Tateno Hiroshi Kurahara Yuukou Mataki Hiroyuki Shinchi Shoji Natsugoe | 2009 | World Journal of Surgery2009，，9： | 2 |
| 4 | Akt negatively regulates the in vitro lifespan of human endothelial cells via a p53/p21- dependent pathway显示文摘 | Miyauchi H Minamino T Tateno K | 2004 | EMBO J2004，23，1： | 1 |
| 5 | Accuracy of estimated phylogenetic tree from molecular data显示文摘 | NEI M TAJIMA F TATENO Y | 1983 | J Mol Evol1983，19，： | 1 |
| 6 | Applicability and limitation of optimal biomass allocation models: a test of two species from fertile and infertile habitats显示文摘 | Osone Y Tateno M | 2005 | Annals of Botany2005，95，： | 1 |
| 7 | Applicability and limitation of optimal biomass allocation models: a test of two species from fertile and infertile habitats显示文摘 | Yoko Osone Masaki Tateno | 2005 | Annals of Botany2005，95，： | 1 |
| 8 | Distinct systemic dis- tribution of salusin-alpha and salusin-beta in the rat 显示文摘 | SUZUKI N SHIHIRI M TATENO T | 2011 | Pep- tides2011，32，4： | 1 |
| 9 | The PI3BC/AKT/mTOR path-way in the pathophysiology and treatment of pituitary adenomas 显示文摘 | Monsalves E’Jurasczhka K Tateno T | 2014 | En-docr Relat Cancer2014，21，4： | 1 |
| 10 | The structure of iron in Earth's inner core显示文摘 | Tateno S Hirose K Ohishi Y | 2010 | Science2010，330，359： | 1 |
| 11 | Above-and belowground biomass and net primary production in a cool-temperate deciduous forest in relation to topographical changes in soil nitrogen 显示文摘 | TATENO R HISHI T TAKEDA H | 2004 | Forest Ecology and Management2004，193，3： | 1 |
| 12 | Ras induces vascular smooth muscle cell senescence and inflammation in human atherosclerosis显示文摘 | Minamino T Yoshida T Tateno K | 2003 | Circulation2003，108，： | 1 |
| 13 | Display of active enzymes on the cell surface of Escherichia coli using PgsA anchor protein and their application to bioconversion显示文摘 | Narita J Okano K Tateno T | 2006 | Appl Microbiol Biotechnol2006，70，5： | 1 |
| 14 | Quantitative analysis of brain perfusion SPECT in Alzheimer's disease using a fully automated regional cerebral blood flow quantification software,3DSRT显示文摘 | Seiju Kobayashi Masaru Tateno Kumiko Utsumi | | 0，，01： | 1 |
| 15 | Multi-agent simula- tion of component reuse focusing on variations in user pref- erence显示文摘 | Kondoh S Tateno T Matsumoto M | 2009 | Cirp Journal of Manufacturing Science and Technology2009，1，4： | 1 |
| 16 | Threshold firing frequency-current relationships of neurons in rat somatosensot cortex: Type 1 and type 2 dynamics显示文摘 | Tateno T Harsch A Robinson H P C | 2004 | Journal of Neurophysiology2004，92，4： | 1 |
| 17 | A novel strategy for mammalian cell surface glyeome profiling using lectin microarray显示文摘 | TATENO H UCHIYAMA N KUNO A | 2007 | Glycobiology2007，17，10： | 1 |
| 18 | 显示文摘 | Ogura M Shinomiya S Tateno J | 2000 | Chem Lett2000，882，： | 1 |
| 19 | Growth potential and differentition capacity of adult rat hepatocyte in vitro显示文摘 | Tateno C Yoshizato K | 1999 | Wound Repair Regen1999，7，1： | 1 |
| 20 | Testicular histology in experimental uremic rats显示文摘 | Adachi Y Sasagawa I Tateno T | 1998 | Arch Androl1998，41，： | 1 |
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