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| 1 | Mammary gland growth and vascularity at parturition and during lactation in primiparous ewes fed differing levels of selenium and nutritional plane during gestation显示文摘Background: Objectives were to examine the effects of selenium(Se) supply and maternal nutritional plane during gestation on mammary gland growth, cellular proliferation, and vascularity at parturition and d 20 of lactation. Rambouillet primiparous ewes(n = 84) were allocated to treatments in a 2 x 3 factorial. Factors were dietary Se(adequate Se [ASe, 11.5 μg/kg BW] or high Se [HSe, 77.0 μg/kg BW]) and nutritional plane(60% [RES], 100% [CON], or 140% [EXC]). At parturition, lambs were removed and 42 ewes(7/treatment) were necropsied. Remaining ewes were fed a common diet meeting requirements for lactation and mechanically milked twice daily until necropsy on d 20. At both necropsy periods, mammary glands were dissected and tissues harvested. Samples were analyzed for RNA, DNA, and protein content, cell proliferation, and vascularity. Where interactions were present(P ≤ 0.05), least squares means from the highest-order interaction are presented. Results: Final body weight of ewes was least(P ≤ 0.002) in RES, intermediate for CON, and greatest for EXC, regardless of stage of the ewe at necropsy(parturition or d 20 of lactation). In ewes necropsied at parturition, mammary glands were heavier(P = 0.02) in EXC compared to RES, with CON intermediate. Concentration of RNA(mg/g) was decreased(P = 0.01) in EXC compared to CON at parturition. There was a tendency(P = 0.07) for a Se by nutrition interaction in percentage of cells proliferating where ASe-EXC ewes had greater(P ≤ 0.02) number of proliferating cells then all other treatments. Mammary vascular area tended(P = 0.08) to be affected by a Se by nutrition interaction where ASe-CON had less(P = 0.007) vascular area than HSe-CON ewes. In ewes necropsied at d 20 of lactation, the number of alveoli per area was decreased(P ≤ 0.05) in RES compared to CON and EXC-fed ewes. Conclusions: Results of this study indicate that proper maternal nutritional plane during gestation is important for mammary gland development, even out to d 20 of lactation. | Tammi L Neville Allison M Meyer Arshi Reyaz Pawel B Borowicz Dale A Redmer Lawrence P Reynolds Joel S Caton Kimberly A Vonnahme | 2013 | Journal of Animal Science and Biotechnology2013，4，4： | 3 |
| 2 | Reactive oxygen species contribute to epidermal hyaluronan catabolism in human skin organ culture显示文摘 | Agren U M Tammi R H Tammi M I | 1997 | Free Radic Biol Med1997，23，7： | 2 |
| 3 | Hyaluronan in human tumors:pathobiological and prognostic messages from cellassociated and stromal hyaluronan显示文摘 | Tammi R H Kultti A Kosma V M | 2008 | Semin Cancer Biol2008，18，： | 1 |
| 4 | Cloning of a human parvovirus by molecular screening of respiratory tract samples 显示文摘 | Allander T Tammi MT Eriksson M | 2005 | Proc Natl Acad Sci USA2005，102，12： | 1 |
| 5 | Cloning of a human parvovirus by molecular screening of respiratory tract samples显示文摘 | Allander T Tammi MT Eriksson M | 2005 | Proe Natl Acad Sci U S A2005，102，12： | 1 |
| 6 | Hyaluronan accumulation in wounded epidermis: a mediator of keratinocyte activation 显示文摘 | Tammi R H Tammi M I | 2009 | J Invest Dermatol2009，129，8： | 1 |
| 7 | Hyaluronan synthases显示文摘 | Weigel P H Hascall V C Tammi M | 1997 | J Biol Chem1997，272，13： | 1 |
| 8 | Cloning of a human par- vovirus by molecular screning of respiratory tract samples 显示文摘 | Allander T Tammi MT Eriksson M | 2005 | NatlA- cad SCI USA2005，102，12： | 1 |
| 9 | Cloning of a hu- man parvovirus by molecular screening of respiratory tract samples显示文摘 | Allander T Tammi M T Erikssan M | 2005 | Proc Natl Acad Sci USA2005，102，12： | 1 |
| 10 | Abnormal fertilization is responsible for reduced fecundity following thiram-induced ovulatory delay in the rat显示文摘 | Tammy E Stoker Susan C Jeffay Robert M Zucker | 2003 | BIOLOGY OF REPRODUCTION2003，68，： | 1 |
| 11 | Cloning of a human parvovirus by molecular screening of respiratory tract samples显示文摘 | Allander T Tammi M T Eriksson M | 2005 | Proc Natl Acad Sci U S A2005，102，12： | 1 |
| 12 | Quantum artificial neural network architectures and components显示文摘 | AJIT N and TAMMY M | 2000 | Information Sciences2000，128，3： | 1 |
| 13 | Cloning of a human parvovirus by molecular screening of respiratory tract samples 显示文摘 | Allandcr T Tammi MT Eriksson M | 2005 | Proc Natl Acad Sci USA2005，102，12： | 1 |
| 14 | Microspectrophotometric quantitation of gly cosaminoglycans in articular cartilage sections stained with safranin O显示文摘 | Kiviranta I Jurvelin J Tammi M | 1985 | Histochemistry1985，82，3： | 1 |
| 15 | Cloning of a human parvovirus by molecular screening of respiratory tract samples 显示文摘 | Allander T Tammi MT Eriksson M | 2005 | Proc Natl Acad Sci USA2005，102，12： | 1 |
| 16 | Cloning of a human parvovirus by molecular screening of respiratory tract samples显示文摘 | Allander T Tammi MT Eriksson M Bjerkner A Tiveljung-Lin- dell A Andersson B | 2005 | Proc Natl Acad Sci U S A2005，102，12： | 1 |
| 17 | Hyaluronan-cell interactions in cancer and vascular disease 显示文摘 | TOOLE B WIGHT T TAMMI M | 2002 | Biol Chem2002，277，7： | 1 |
| 18 | Cloning of a human parvovirus by molecular screening of respiratory tract samples 显示文摘 | Allander T Tammi MT Eriksson M | 2005 | Proc Natl Acad Sci2005，102，12： | 1 |
| 19 | Developmentally programmed expression of hyaluronan in human skin and its appendages显示文摘 | Tammi M Ryynanen M | 1997 | J Invest Dermatol1997，109，2： | 1 |
| 20 | Hyaluronan-cell in- teractions in cancer and vau|ar disea 显示文摘 | TOOLE B P WIGHT T N TAMMI M I | 2002 | J Biol Chem2002，277，7： | 1 |
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