| 2 | Inhibitory effects of safranal on laser-induced choroidal neovascularization and human choroidal microvascular endothelial cells and related pathways analyzed with transcriptome sequencing显示文摘AIM:To determine the effects of safranal on choroidal neovascularization(CNV)and oxidative stress damage of human choroidal microvascular endothelial cells(HCVECs)and its possible mechanisms.METHODS:Forty-five rats were used as a laser-induced CNV model for testing the efficacy and safety of safranal(0.5 mg/kg·d,intraperitoneally)on CNV.CNV leakage on fluorescein angiography(FA)and CNV thickness on histology was compared.HCVECs were used for a H_(2)O_(2)-induced oxidative stress model to test the effect of safranal in vitro.MTT essay was carried to test the inhibition rate of safranal on cell viability at different concentrations.Tube formation was used to test protective effect of safranal on angiogenesis at different concentrations.mRNA transcriptome sequencing was performed to find the possible signal pathway.The expressions of different molecules and their phosphorylation level were validated by Western blotting.RESULTS:On FA,the average CNV leakage area was 0.73±0.49 and 0.31±0.11 mm^(2)(P=0.012)in the control and safranal-treated group respectively.The average CNV thickness was 127.4±18.75 and 100.6±17.34μm(P=0.001)in control and safranal-treated group.Under the condition of oxidative stress,cell proliferation was inhibited by safranal and inhibition rates were 7.4%-35.4%at the different concentrations.For tube formation study,the number of new branches was 364 in control group and 35,42,and 17 in 20,40,and 80μg/mL safranal groups respectively(P<0.01).From the KEGG pathway bubble graph,the PI3K-AKT signaling pathway showed a high gene ratio.The protein expression was elevated of insulin receptor substrate(IRS)and the phosphorylation level of PI3K,phosphoinositide-dependent protein kinase 1/2(PDK1/2),AKT and Bcl-2 associated death promoter(BAD)was also elevated under oxidative stress condition but inhibited by safranal.CONCLUSION:Safranal can inhibit CNV both in vivo and in vitro,and the IRS-PI3K-PDK1/2-AKT-BAD signaling pathway is involved in the pathogenesis of CNV. | Qin-Xiao Yao-Yao Sun Zhan-Jun Lu Tian-Zi Zhang Shan-Shan Li Ting Hua Suriguga Wen-Lin Chen Lin-Lin Ran Wen-Zhen Yu Fei Yang Burenbatu | 2021 | International Journal of Ophthalmology(English edition)2021，14，7： | 1 |
| 3 | HPLC analysis of 16 compounds from Artemisia ordosica显示文摘Objective: To establish a high-performance liquid chromatographic method(HPLC) for the simultaneous determination of 16 compounds from Artemisia ordosica.Methods: HPLC was used to analyze 16 quality indicators of A. ordosica. The HPLC conditions were as follows: Agilent Eclipse Plus C18column(250 mm × 4.6 mm, 5 μm) with acetonitrile(A)-water(B) as mobile phase, gradient elution: 0–10 min, 75%-65% B;10–30 min, 65%-35% B;and finally 30–40 min, 35%-15%B. The flow rate was 1.0 mL/min, the column temperature was 40 °C, the injection volume was 10 μL, and monitored by absorbance at 285 nm for compounds 1–10, 12 and 225 nm for compounds 11, 13–16.Results: Under the selected experimental chromatographic conditions, compounds 1–16 showed good linearity(r > 0.9993) in a wide concentration range. Their average recoveries were 99.50%, 95.38%,97.75%, 96.00%, 98.20%, 97.50%, 95.50%, 99.33%, 96.75%, 96.50%, 98.50%, 97.83%, 99.20%, 95.33%, 97.33%and 96.30%, respectively, and the RSD were 1.99%, 1.81%, 1.63%, 1.98%, 1.67%, 1.92%, 1.74%, 1.67%,1.90%, 1.72%, 1.88%, 1.83%, 1.79%, 1.76%, 1.81% and 1.96%, respectively.Conclusion: Based on the results of the HPLC analysis, it was concluded that p-hydroxycinnamic acid(1),O-hydroxycinnamic acid(2), coniferyl alcohol(5), 5,4’-dihydroxy-7,3’-dimethoxyflavanone(8), 5,4’-dihy droxy-7-methoxyflavanone(9), 5-hydroxy-7,4’-dimethoxyflavanone(12), dehydrofalcarindiol(13), arteordoyn A(14), dehydrofalcarinol(15) and capillarin(16) are best suited for the role of quality indicators of A. ordosica grown in different ecological environments. | Xiurong Kang Qinghu Wang Suriguga Ao Moxiyele Wenlin Bao Chaoliang Zhao | 2023 | Chinese Herbal Medicines2023，15，2： | 0 |
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