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| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | Comparison of the biologic effects of MA5 and B-B4 monoclonal antibody labeled with iodine-131 and bismuth-213 on multiple myeloma显示文摘 | Supiot S Faivre Chauvet A Couturier O | 2002 | Cancer2002，94，4： | 1 |
| 3 | Current state of knowledge regarding the use of antiangiogenic agents with radiation therapy 显示文摘 | MAZERON R ANDERSON B SUPIOT S | 2011 | Cancer Treat Rev2011，37，6： | 1 |
| 4 | Migraine in 2009:from attack to treatment显示文摘 | Supiot F | | 0，，04： | 1 |
| 5 | Dosimetry results suggest feasibility of radioimmunotherapy using anti-CD138 (B-B4) antibody in multiple myeloma patients显示文摘 | Rousseau C Ferrer L Supiot S | 2012 | Tumour Biol2012，33，3： | 1 |
| 6 | Migraine in 2009: from attack to treatment显示文摘 | SuPiot F | 2009 | Rev Med Brux2009，30，4： | 1 |
| 7 | Bilaeral vocal fold pal sy caused by chronic motor axonal neuropathy显示文摘 | Marchant H Supiot F Choufani G | 2003 | Larygol Otol2003，117，： | 1 |
| 8 | Mechanisms of cell sensitization to alpha radioimmunotherapy by doxorubicin or paclitaxel in multiple myeloma cell lines 显示文摘 | SUPIOT S GOUARD S CHARRIER J | 2005 | Clin Cancer Res2005，11，192： | 1 |
| 9 | Migraine in 2009:from attack to treatmem显示文摘 | SuPiot F | 2009 | Rev Med Brux2009，30，4： | 1 |
| 10 | Mechanisms of cellsensitization to alpha radioimmunotherapy by doxorubicin or pacli-taxel in multiple myeloma cell lines显示文摘 | Supiot S Gouard S Charrier J | 2005 | Clin Cancer Res2005，11，192： | 1 |
| 11 | Mechanisms of cell sensitization to alpha radioimmunotherapy by doxorubicin or paclitaxel in multiple myeloma cell lines显示文摘 | Supiot S Gouard S Charrier J | 2005 | Clin Cancer Res2005，19，2： | 1 |
| 12 | Nutlin-3 radiosensitizes hypoxic prostate cancer cells independent of p53显示文摘 | Supiot S Hill R P Bristow R G | 2008 | Mol Cancer Ther2008，7，4： | 1 |
| 13 | Elaboration of fire retardant coating on polyamide-6 using a cold plasma polymerization process显示文摘 | Quédé A Jama C Supiot P | 2002 | Surf Coat Technol2002，，： | 1 |
| 14 | Characterization of organosilicon films synthesized by N2-PAVCD:Application to fire retardant properties of coated polymers显示文摘 | Quédé A Mutel B Supiot P | 2004 | Surf Coat Techno12004，，： | 1 |
| 15 | Dosimetry results suggest feasibility of radioimmunotherapy using anti-CD138 (B-B4) antibody in multiple myeloma patients显示文摘 | Rousseau C Ferrer L Supiot S | 2012 | Tumour Biol2012，33，： | 1 |
| 16 | Characterization of organosilicon films synthesized by N - PACVD 显示文摘 | Quédé A Mutel B Supiot P | 2004 | Surface and Coatings Technology2004，，180181： | 1 |
| 17 | Stereotactic body radiation therapy for abdominal oligometastases:a biological and clinical review显示文摘 | Almaghrabi MY Supiot S Paris F | 2012 | Radiat Oncol2012，7，： | 1 |
| 18 | Characterization of organosilicon films synthesized by N-PACVD application to fire retardant properties of coated polymers显示文摘 | A Quede B Mutel P Supiot | 2004 | Surface and Coatings Technology2004，，： | 1 |
| 19 | Stereotactic body radiation therapy for abdominal oligometastases: a biological and clinical review 显示文摘 | Almaghrabi MY Supiot S Paris F | 2012 | Radiat Oncol2012，7，1： | 1 |
| 20 | Density and Temperature of N Atoms in the Afterglow of a Microwave Discharge Measured by a Two-photon Laser-induced Fluorescence Technique 显示文摘 | Mazouffre S Foissac C Supiot P | 2001 | Plasma Sources Science & Technology2001，10，2： | 1 |
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