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| 1 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 2 | Factors affecting choice of community residential care setting 显示文摘 | Sales AE Hedrick SC Sullivan J | 2005 | J Aging Health2005，17，2： | 1 |
| 3 | bHLH-PAS proteins in cancer显示文摘 | Bersten DC Sullivan AE Peet DJ | 2013 | Nat Rev Cancer2013，13，12： | 1 |
| 4 | Interleukin-6 induces an epithelial mesenchymal transition phenotype in human breast cancer cells显示文摘 | Sullivan NJ Sasser AK Axel AE | 2009 | Oncogene2009，28，： | 1 |
| 5 | Recurrent fetal aneuploidy and recurrent miscarriage 显示文摘 | Sullivan AE Silver RM LaCoursiere DY | 2004 | Obstetri Gyneeol2004，104，4： | 1 |
| 6 | Decrease in fibrin content of venous thrombi in selectin-deficient mice 显示文摘 | Sullivan VV Hawley AE Farris DM | 2003 | J Surg Res2003，109，1： | 1 |
| 7 | Interleukin-6 induces an epi- thelial-mesenchymal transition phenotype in human breast cancer cells 显示文摘 | Sullivan NJ Sasser AK Axel AE | 2009 | Oneogene2009，28，33： | 1 |
| 8 | Interleukin-6 induces an epithelial-mesenchymal transition phenotype in human breast cancer cells显示文摘 | Sullivan NJ Sasser AK Axel AE | 2009 | Oncogene2009，28，33： | 1 |
| 9 | Decrease in fibrin content of venous thrombi in selectin-deficient mice显示文摘 | Sullivan VV Hawley AE Farris DM | 2003 | J Surg Res2003，109，： | 1 |
| 10 | Decrease in fibrin content of venous thrombi in selectin - deficient mice 显示文摘 | Sullivan W Hawley AE Farris DM | 2003 | J Surg Res2003，109，1： | 1 |
| 11 | Interleukin-6 induces an epithelial mesenchymaI transition pbenotype in human breast cancer cells 显示文摘 | Sullivan N J Sasser AK Axel AE | 2009 | Oncogene2009，28，： | 1 |
| 12 | Interleukin-6 induces an epithelial-mesenchymal transition phenotype in human breast canc- er ceils 显示文摘 | Sullivan NJ Sasser AK Axel AE | 2009 | Oncogene2009，28，33： | 1 |
| 13 | Recurrentfetal aneuploidy and recurrent miscarriage显示文摘 | Sullivan AE Silver RM LaCoursiere DY | 2004 | ObstetGynecol2004，104，4： | 1 |
| 14 | Interleukin-6 in- duces an epithelial mesenchymal transition phenotype in human breast cancer cells 显示文摘 | Sullivan NJ Sasser AK Axel AE | 2009 | Oncogene2009，28，33： | 1 |
| 15 | bHLH - PAS proteins in cancer 显示文摘 | Bersten DC Sullivan AE Peet DJ | 2013 | Nature Reviews Cancer2013，13，12： | 1 |
| 16 | Systemically dispersed innate IL-13-expressing cells in type 2 immunity 显示文摘 | Price AE Liang HE Sullivan BM | 2010 | Proc Natl Acad Sci U S A2010，107，11： | 1 |
| 17 | The manage- ment of acardiac twins : A conservative approach 显示文摘 | Sullivan AE Varner MW Ball RH | 2003 | Am J Obstet Gyneco12003，189，5： | 1 |
| 18 | Systemically dispersed innate IL-13-expressing cells in type 2 immunity显示文摘 | Price AE Liang HE Sullivan BM | 2010 | Proc Natl Acad Sci USA2010，107，11： | 1 |
| 19 | Recurrent lumbar disc protrusion显示文摘 | O' Sullivan MG Connolly AE Buckley TF | 1990 | Br J Neurosurg1990，4，： | 1 |
| 20 | Systemically dispersed innate IL-13-expressing cells in type 2 immunity 显示文摘 | Price AE Liang HE Sullivan BM | 2010 | Proc Nail Acad Sci USA2010，107，11： | 1 |
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