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| 1 | Cytotoxic and antibacterial substances against multi-drug resistant pathogens from marine sponge symbiont:Citrinin,a secondary metabolite of Penicillium sp.显示文摘Objective:To Isolate,purify,characterize,and evaluate the bioaclive compounds from the sponge-derived fungus Penicillium sp.FF001 and to elucidate its structure.Methods:The fungal strain FF001 with an interesting bioactivity profile was isolated from a marine Fijian sponge Melophlus sp.Based on conidiophores aggregation,conidia development and mycelia morphological characteristics,the isolate FF001 was classically identified as a Penicillium sp.The bioactive compound was identified using various spectral analysis of UV,high resolution electrospray ionization mass spectra,1H and 13C NMR spectral data.Further minimum inhibitory concentrations(MICs)assay and brine shrimp cytotoxicity assay were also carried out to evaluate the biological properties of the purified compound.Results:Bioassay guided fractionation of the EtOAc extract of a static culture of this Penicillium sp.by different chromatographic methods led the isolation of an antibacterial,anticryptococcal and cytotoxic active compound,which was identified as citrinin(1).Further,citrinin(1)is reported for its potent antibacterial activity against methicillin-resistant Staphylococcus aureus(S.aureus),rifampicin-resistant 5.aureus,wild type S.aureus and vancomycin-resistant Enterococcus faecium showed MICs of 3.90,0.97,1.95 and7.81μg/mL,respectively.Further citrinin(1)displayed significant activity against the pathogenic yeast Cryptococcus neoformans(MIC 3.90μg/mL),and exhibited cytotoxicity against brine shrimp larvae LD_(50)of 96μg/mL.Conclusions:Citrinin(1)is reported from sponge associated Penicillium sp.from this study and for its strong antibacterial activity against multi-drug resistant human pathogens including cytotoxicity against brine shrimp larvae,which indicated that sponge associated Penicillium spp.are promising sources of natural bioactive metabolites. | Ramesh Subramani Rohitesh Kumar Pritesh Prasad William Aalbersberg | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，4： | 6 |
| 2 | Two Distinct Fluorescent Quantum Clusters of Gold Starting from Metallic Nanoparticles by pH-Dependent Ligand Etching显示文摘Two fl uorescent quantum clusters of gold,namely Au25 and Au8,have been synthesized from mercaptosuccinic acid-protected gold nanoparticles of 45 nm core diameter by etching with excess glutathione.While etching at pH~3 yielded Au_(25),that at pH 7-8 yielded Au_(8).This is the fi rst report of the synthesis of two quantum clusters starting from a single precursor.This simple method makes it possible to synthesize well-defined clusters in gram quantities.Since these clusters are highly fl uorescent and are highly biocompatible due to their low metallic content,they can be used for diagnostic applications. | Madathumpady Abubaker Habeeb Muhammed Subramani Ramesh Sudarson Sekhar Sinha Samir Kumar Pal Thalappil Pradeep | 2008 | Nano Research2008，1，4： | 5 |
| 3 | Marine actinomycetes: An ongoing source of novel bioactive metabolites显示文摘 | Ramesh Subramani William Aalbersberg | 2012 | Microbiological Research2012，，10： | 3 |
| 4 | Characterization of a thermostable alkaline protease produced by marine Streptomyces?fungicidicus MML1614显示文摘 | Subramani Ramesh Mahalingam Rajesh Narayanasamy Mathivanan | 2009 | Bioprocess and Biosystems Engineering2009，，6： | 2 |
| 5 | Marine actinomycetes: An ongoing source of novel bioactive metabolites显示文摘 | Ramesh Subramani William Aalbersberg | 2012 | Microbiological Research2012，，10： | 2 |
| 6 | Culturable rare Actinomycetes : diversity, isolation and marine natural product discovery显示文摘 | Ramesh Subramani William Aalbersberg | 2013 | Applied Microbiology and Biotechnology2013，，21： | 1 |
| 7 | Scre-ening of marine actinomycetes isolated from the Bayof Bengal,India for antimicrobial activity and industri-al enzymes December显示文摘 | Subramani Ramesh Narayanasamy Mathivanan | 2009 | World Journal of Microbiol-ogy and Biotechnology2009，25，12： | 1 |
| 8 | Hydrographical parameters and phytoplankton assemblages along the Pondicherry—Nagapattinam coastal waters,southeast coast of India显示文摘Hydrographical parameters and phytoplankton assemblages were determined along the Pondicherry, Parangipettai and Nagapattinam coastal waters, southeast coast of India. All the hydrographical parameters such as sea surface temperature, salinity, p H, total alkalinity, dissolved oxygen and nutrients like nitrate, nitrite, inorganic phosphate and reactive silicate, chlorophyll a and phytoplankton assemblages were studied for a period of five months(May, August, September 2010, March and November 2011). Over 121 species of phytoplankton represented by 93 species of diatoms, 16 species of dinoflagellates, 9 species of blue-green algae, 2 species of greens and 1 species of silicoflagellate were recorded. High phytoplankton species diversity was found in March 2011 when salinity and nitrate concentrations were low and reactive silicate and inorganic phosphates were moderate. Species diversity was low during May 2010 when increased sea surface temperature, salinity and low nutrients availability were observed. Coscinodiscus centralis, Diatoma vulgaris and Proboscia alata were dominant, especially Coscinodiscus sp. distributed in all stations whereas Skeletonema costatum, Odontella sinensis and Ditylum brightwellii were abundant in August and September 2010. From principal component analysis and multiple regression analysis, it is evident that variables like sea surface temperature and dissolved oxygen are the most important factors influencing the seasonal pattern of phytoplankton population. | Pitchai SAMPATHKUMAR Srinivasan BALAKRISHNAN Krishnamoorthy KAMALAKANNAN Rethinavelu SANKAR Lakshmanan RAMKUMAR Subramani RAMESH Neelamegam KABILAN Thambusamy SURESHKUMAR Chellam THENMOZHI Mookapillai GOPINATH Sekar JAYASUDHA Amalanathan AROKIYASUNDRAM Thiruvarasan LENIN Thangavelu BALASUBRAMANIAN | 2015 | Advances in Climate Change Research2015，6，1： | 0 |
| 9 | Production and purification of a bioactive substance against multi-drug resistant human pathogens from the marine-sponge-derived Salinispora sp.显示文摘Objective:To isolate,purify,characterize,elucidate structure and evaluate bioactive compounds from the sponge-derived Salinispora sp.FS-0034.Methods:The symbiotic actinomycete strain FS-0034 with an interesting bioactivity profile was isolated from the Fijian marine sponge Theonella sp.Based on colony morphology and obligatory requirement of seawater for growth,and mycelia morphological characteristics the isolate FS-0034 was identified as a Salinispora sp.The bioactive compound was identified by using various spectral analysis of ultraviolet,high resolution electrospray ionization mass spectroscopy,H nuclear magnetic resonance,correlated spectroscopy and heteronuclear multiple bond coherence spectral data.A minimum inhibitory concentration assay were performed to evaluate the biological properties of the pure compound against multi-drug resistant pathogens.Results:Bioassay guided fractionation of the ethyl acetate extract of the culture of Salinispora sp.FS-0034 by different chromatographic methods yielded the isolation of an antibacterial compound,which was identified as rifamycin W(compound 1).Rifamycin W was reported for its potent antibacterial activity against methicillin-resistant Staphylococcus aureus,wild type Staphylococcus aureus and vancomycin-resistant Enterococcus faecium and displayed minimum inhibitory concentrations of 15.62,7.80 and 250.00 μg/mL,respectively.Conclusions:The present study reported the rifamycin W from sponge-associated Salinispora sp.and it exhibited appreciable antibacterial activity against multi-drug resistant human pathogens which indicated that sponge-associated Actinobacteria are significant sources of bioactive metabolites. | Satyendra Singh Pritesh Prasad Ramesh Subramani William Aalbersberg | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，10： | 0 |
| 10 | Sprouted Sorghum Extract Elicits Coleoptile Emergence, Enhances Shoot and Root Acclimatization, and Maintains Genetic Fidelity in indica Rice显示文摘The high growth-stimulating effect of plant extract has urged the plant biotechnologists to use natural supplements in the culture media instead of synthetic phytohormones. We advocated the effect of sprouted sorghum extract(SSE) on emergence, in vitro acclimatization, and genetic fidelity in coleoptile derived callus of indica rice variety ADT36. The use of SSE with Murashige Skoog medium efficiently acclimatized the root and shoot apical systems. A higher mat and seminal roots(3.4 g biomass) with an efficient shoot primordium elongation were observed with an increase in the concentration of SSE. Seeds treated with SSE medium showed higher germination and earlier coleoptile maturation about 48 h compared to untreated seeds, and there was a higher expression of e EF-1α with an increase in coleoptile length. B5 medium was effective on inducing embryogenic and nodular callus from 3-day-old coleoptile with 3.0 mg/L 2,4-dichlorophenoxyacetic acid and further proliferated effectively with 0.8 mg/L kinetin with a fresh weight of 180 mg. Highly significant regeneration was observed with combination of 2.5 mg/L 6-benzylamino purine and 3.0 mg/L α-naphthaleneacetic acid. The metabolic and genetic profiles of in vitro and directly cultivated plants were the same, examined through Fourier-transform infrared spectroscopy, random amplified polymorphic DNA(RAPD), inter-simple sequence repeat(ISSR) and R-ISSR(combination of RAPD and ISSR) markers, respectively, and thus confirming the significant efficacy of the SSE incorporated medium. Disarmed T-DNA was transformed to coleoptile derived callus through Agrobacterium tumefaciens LBA4404 and confirmed by GUS assay. The T-DNA integration was confirmed by DNA blot analysis using DNA from transient GUS-expressed explants. Thus, SSE can be used as a natural and organic supplement for organogenesis and efficient acclimatizations of shoot and root apical meristems in regenerated plants. | Subramanian Radhesh KRISHNAN Pandiyan MUTHURAMALINGAM Subramani PANDIAN Ramachandradoss BANUPRIYA Gunasekar CHITHRA Manikandan RAMESH | 2018 | Rice science2018，25，2： | 0 |
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