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| 1 | Mucosal bacterial microflora and mucus layer thickness in adolescents with inflammatory bowel disease显示文摘AIM:To assess the mucosa-associated bacterial microflora and mucus layer in adolescents with inflammatory bowel disease(IBD) .METHODS:Sixty-one adolescents(mean age 15 years,SD ± 4.13) were included in the study.Intestinal biopsies from inflamed and non-inflamed mucosa of IBD patients and from controls with functional abdominal pain were cultured under aerobic and anaerobic conditions.The number of microbes belonging to the same group was calculated per weight of collected tissue.The mucus thickness in frozen samples was measured under a fluorescent microscope.RESULTS:The ratios of different bacterial groups in inflamed and non-inflamed mucosa of IBD patients and controls were specific for particular diseases.Streptococcus spp.were predominant in the inflamed mucosa of Crohn's disease(CD) patients(80% of all bacteria) ,and Lactobacillus spp.were predominant in ulcerative colitis patients(90%) .The differences were statistically significant(P = 0.01-0.001) .Lower number of bifidobacteria was observed in the whole IBD group.A relation was also found between clinical and endoscopic severity and decreased numbers of Lactobacillus and,to a lesser extent,of Streptococcus in biopsies from CD patients.The mucus layer in the inflamed sites was significantly thinner as compared to controls(P = 0.0033) and to non-inflamed areas in IBD patients(P = 0.031) .CONCLUSION:The significantly thinner mucosa of IBD patients showed a predominance of some aerobes specific for particular diseases,their numbers decreased in relation to higher clinical and endoscopic activity of the disease. | Krzysztof Fyderek Magdalena Strus Kinga Kowalska-Duplaga Tomasz Gosiewski Andrzej Wedrychowicz Urszula Jedynak-Wasowicz Malgorzata Sladek Stanislaw Pieczarkowski Pawel Adamski Piotr Kochan Piotr B Heczko | 2009 | World Journal of Gastroenterology2009，15，42： | 14 |
| 2 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 3 | Growth stimulatory actions of insulin in vitro and in vivo显示文摘 | STRUS D S | 1984 | Endocr Rev1984，12，5： | 1 |
| 4 | Rise of blood pressure with periodic limb movements in sleep and wakefulness显示文摘 | Siddiqui F Strus J Ming X | 2007 | Clinical Neurophysiology2007，118，9： | 1 |
| 5 | Effect of Zr on the microstructure,mechanical properties and corrosion resistance of Mg-10Gd-3Y magnesium alloy显示文摘 | Stru M Wu G H Wang W | 2009 | Materials Science and Engineering A2009，523，12： | 1 |
| 6 | The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida 显示文摘 | Strus M Kucharska A Kukla G | 2005 | Infect Dis Obstct Gynecol2005，13，2： | 1 |
| 7 | The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida显示文摘 | STRUS M KUCHARSKA A KUKLA G | 2005 | In- fect Dis Obstet & Gyneco12005，13，2： | 1 |
| 8 | Effects of developmental care on behavioral organization in verylow-birth weight infant显示文摘 | Becker P T Grunwald P C Moorman J Stru HR S | 1993 | Nursing Research1993，，42： | 1 |
| 9 | In vitro antagonistic effect of Lactobacilli on organisms associated with bacterial vaginosis 显示文摘 | STRUS M MALINOWSKA M HECZKO P B | 2002 | J Report Med2002，47，1： | 1 |
| 10 | Recommended practices for cleaning and caring for surgical instruments and powered equipment显示文摘 | Strus M | 2002 | AORN2002，75，3： | 1 |
| 11 | The in vitro activity of va- ginal Lactobacillus with probiotic properties against Candida显示文摘 | Strus M Kucharska A Kukla G | 2005 | Infect Dis Obstet Gynecol2005，13，2： | 1 |
| 12 | The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida 显示文摘 | Strus M Kucharska A Kukla G | 2005 | Infect Dis Obstet Gynecol2005，13，2： | 1 |
| 13 | Structural analysis of the Lactobacillus rhamnosus strain KL37C exopolysacclaaride 显示文摘 | Lipinski T Jones C Lemercinier X Korzeniowska-Kowal A Strus M Rybka J Gamian A Heczko PB | 2003 | Carbohydr Res2003，338，7： | 1 |
| 14 | Strain energy and lateral friction force distributions of carbon nanotubes manipulated into shapes by atomic force microscopy显示文摘 | STRUS M LAHIJI R ARES P | 2009 | Nanotechnology2009，20，38： | 1 |
| 15 | Rise of blood pressure with periodic limb movements in sleep and wakefulness显示文摘 | Siddiqui F Strus J Ming X | 2007 | Clin Neurophysiol2007，118，9： | 1 |
| 16 | Inhibitory activity of vaginal Lactobacillus bacteria on yeasts causing vulvovaginal candidiasis显示文摘 | STRUS M BRZYCHCZY-WIOCH M KUCHARSKA A | 2005 | Med Diagn Microbiol2005，57，1： | 1 |
| 17 | Rise of blood pressure with periodic limb movements in sleep and wakefulness显示文摘 | Siddiqui F Strus J Ming X | 2007 | Clin Neurophysiol2007，118，9： | 1 |
| 18 | The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida显示文摘 | STRUS M KUCHARSKA A KUKLA G | 2005 | Infect Dis Obstet Gynecol2005，13，2： | 1 |
| 19 | Aldosterone: cardiovascular assault显示文摘 | STRU'IERS A D | 2002 | Amer- ican Heart Journal2002，144，5： | 1 |
| 20 | Dysfunctional glia:contributors to neurodegenerative disorders显示文摘Astrocytes are integral components of the central nervous system,where they are involved in numerous functions critical for neuronal development and functioning,including maintenance of blood-brain barrier,formation of synapses,supporting neurons with nutrients and trophic factors,and protecting them from injury.These roles are markedly affected in the course of chronic neurodegenerative disorders,often before the onset of the disease.In this review,we summarize the recent findings supporting the hypothesis that astrocytes play a fundamental role in the processes contributing to neurodegeneration.We focus onα-synucleinopathies and tauopathies as the most common neurodegenerative diseases.The mechanisms implicated in the development and progression of these disorders appear not to be exclusively neuronal,but are often related to the astrocytic-neuronal integrity and the response of astrocytes to the altered microglial function.A profound understanding of the multifaceted functions of astrocytes and identification of their communication pathways with neurons and microglia in health and in the disease is of critical significance for the development of novel mechanism-based therapies against neurodegenerative disorders. | Marta Sidoryk-Węgrzynowicz Lidia Strużyńska | 2021 | Neural Regeneration Research2021，16，2： | 1 |
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