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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Antioxidant and antimicrobial properties of Moltkia petraea (Tratt.) Griseb. flower, leaf and stem infusions显示文摘 | M. Zovko Kon?i? D. Kremer J. Gruz M. Strnad G. Bi?evac I. Kosalec D. ?amec J. Piljac-?egarac K. Karlovi? | 2010 | Food and Chemical Toxicology2010，，6： | 4 |
| 3 | Results of National Colorectal Cancer Screening Program in Croatia(2007-2011)显示文摘AIM:To study the epidemiologic indicators of uptake and characteristic colonoscopic findings in the Croatian National Colorectal Cancer Screening Program.METHODS:Colorectal cancer(CRC) was the second leading cause of cancer mortality in men(n = 1063,49.77/100 000),as well as women(n = 803,34.89/100 000) in Croatia in 2009.The Croatian National CRC Screening Program was established by the Ministry of Health and Social Welfare,and its implementation started in September,2007.The coordinators were recruited in each county institute of public health with an obligation to provide fecal occult blood testing(FOBT) to the participants,followed by colonoscopy in all positive cases.The FOBT was performed by hypersensitive guaiac-based Hemognost card test(Biognost,Zagreb).The test and short questionnaire were delivered to the home addresses of all citizens aged 50-74 years consecutively during a 3-year period.Each participant was required to complete the questionnaire and send it together with the stool specimen on three test cards back to the institute for further analysis.About 4% FOBT positive cases are expected in normal risk populations.A descriptive analysis was performed.RESULTS:A total of 1 056 694 individuals(born between 1933-1945 and 1952-1957) were invited to screening by the end of September 2011.In total,210 239(19.9%) persons returned the envelope with a completed questionnaire,and 181 102 of them returned it with a correctly placed stool specimen on FOBT cards.Until now,12 477(6.9%),FOBT-positive patients have been found,which is at the upper limit of the expected values in European Guidelines for Quality Assurance in CRC Screening and Diagnosis [European Union(EU) Guidelines].Colonoscopy was performed in 8541 cases(uptake 66%).Screening has identified CRC in 472 patients(5.5% of colonoscopied,3.8% of FOBT-positive,and 0.26% of all screened individuals).This is also in the expected range according to EU Guidelines.Polyps were found and removed in 3329(39% of colonoscopied) patients.The largest number of polyps were found in the left half of the colon:64%(19%,37% and 8% in the rectum,sigma,and descendens,respectively).The other 36% were detected in the proximal part(17% in the transverse colon and 19% in ceco-ascending colon).Small polyps in the rectum(5-10 mm in diameter),sigmoid and descending colon were histologically found to be tubular adenomas in 60% of cases,with a low degree of dysplasia,and 40% were classified as hyperplastic.Polyps of this size in the transverse or ceco-ascending colon in almost 20% had a histologically villous component,but still had a low degree of dysplasia.Polyps sized 10-20 mm in diameter were in 43% cases tubulovillous,and among them,32% had areas with a high degree of dysplasia,especially those polyps in the cecoascending or transverse part.The characteristics of the Croatian CRC Screening National Program in the first 3 years were as follows:relatively low percentage of returned FOBT,higher number of FOBT-positive persons but still in the range for population-based programs,and higher number of pathologic findings(polyps and cancers).CONCLUSION:These results suggest a need for intervention strategies that include organizational changes and educational activities to improve awareness of CRC screening usefulness and increase participation rates. | Miroslava Katii Nataa Antoljak Milan Kujundzi Valerija Stameni Dunja Skoko Poljak Danica Kramari Davor timac Marija Strnad Peikan Mirko amija Zdravko Ebling | 2012 | World Journal of Gastroenterology2012，18，32： | 2 |
| 4 | The importance of homnnone receptors in benign breast disease显示文摘 | Strnad P Zavadil M DanesJ | 1998 | Ceska Gynekol1998，63，1： | 1 |
| 5 | Ethylene interacts with acts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidiumsativur and Arabidopsis thaliana 显示文摘 | Linkies A Muller K Morris K Tureckova V Wenk M Cadman CS Corbineau F Strnad M Lynn J R Finch-Savage W E Leubner-Metzger G | 2009 | Plant Cell2009，21，12： | 1 |
| 6 | Study of lymphatic mapping and sentinel node identification in early stage cervical cancer 显示文摘 | Rob L Strnad P Robova H | 2005 | Gynecol Oncol2005，98，： | 1 |
| 7 | Pharmacological characterization of the rat A2a adenosine receptor functionally coupled to the yeast pheromone response pathway 显示文摘 | Price LA Strnad J Pausch MH | 1996 | Mol Pharmacol1996，50，4： | 1 |
| 8 | Arabidopsis cyto- kinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root develop- ment, and cytokinin metabolism 显示文摘 | Riefler M Novak O Strnad M | 2006 | The Plant Cell2006，18，1： | 1 |
| 9 | Keratin 8 overexpression promotes mouse Mallory body formation显示文摘 | NAKAMICHI I TOIVOLA D M STRNAD P | 2005 | J Cell Biol2005，171，6： | 1 |
| 10 | Keratin overexpression levels correlate with the extent of spontaneous pancreatic injury显示文摘 | TOIVOLA D M NAKAMICHI I STRNAD P | 2008 | Am J Pathol2008，172，4： | 1 |
| 11 | Correction: Combination of lung ultrasound (a comet-tail sign)and N-terminal pro-brain natri- uretic peptide in differentiating acute heart failure from chronic ob- structive pulmonary disease and asthma as cause of acute dyspnea in prehospital emergency setting 显示文摘 | Prosen G Klemen P Strnad M | 2011 | Crit Care2011，15，6： | 1 |
| 12 | Regulation of plant growth by cytokinin显示文摘 | Werner T Motyka V Strnad M | 2001 | Proceedings of the National A- cademy of Science of USA2001，98，10： | 1 |
| 13 | Identification ofnovel principles of keratin filament network turnover in living cells显示文摘 | WINDOFFER R WOLL S STRNAD P | 2004 | Mol Biol Cell2004，15，5： | 1 |
| 14 | In vitro haemocompatibility evalu-ation of PET surfaces using the quartz crystal microbalance technique显示文摘 | Dolika A Strnad S Stana J | 2011 | J Biomater Sci Polym Ed2011，，： | 1 |
| 15 | Californium-252 (zsz Cf) versus conventional gamma radiation in the braChytherapy of advanced cervical carcinoma long-term treatment results of a randomized study 显示文摘 | Tacev T Ptackova B Strnad V | 2003 | Strahlenther Onkol2003，179，6： | 1 |
| 16 | The influence ofnoradrenergic blockade on vasospasm and the quantity ofcerebral dopamine beta-hydroxylase following subarachnoidhaemorrhage in rabbits 显示文摘 | BUNC G KOVACIC S STRNAD S | 2003 | Wien Klin Wochenschr2003，115，1718： | 1 |
| 17 | Nucleotide sequence,organization and characterization of the (halo)aromatic acid catabolic plasmid pAS1 from Achromobacter xylosoxidans AS显示文摘 | JENCOVA V STRNAD H CHODORA Z | 2008 | Research in Microbiology2008，159，2： | 1 |
| 18 | Presentation,management and outcome of 32 patients with pregnancy-associated breast cancer: a matched controlled study显示文摘 | Halaska M J Pentheroudakis G Strnad P | 2009 | Breast J2009，15，5： | 1 |
| 19 | Ikappa B kinase inhibitors for treating autoimmune and inflammatory disorders:potential and challenges显示文摘 | STRNAD J BURKE J R | 2007 | Trends Pharmacol Sci2007，28，3： | 1 |
| 20 | The importance of hormone receptorsin benign breast diseases显示文摘 | Strnad P Zavaduk N Daneas J | 1998 | Ceska Gynekol1998，63，1： | 1 |
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