|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Activation of mitogen activated protein kinases via complement receptor type 2显示文摘Background Complement receptor type 2 (CR2) is the receptor for C3d and C3dg and for Epstein Barr virus The aim of our study was to explore whether CR2 can independently mediate the activation of mitogen activated protein kinases (MAPKs, including ERK, JNK, and p38MAPK), and to highlight the molecular mechanism of CD 4 + cell deletion in AIDS Methods HOS cells (HOS CR2) and HOS CD4 cells (HOS CD4CR2) stably expressing CR2 were established and then identified by FACS and Western blotting Activation and blocking tests of MAPKs were assessed by Western blot Cell proliferation was determined using Cell Titer 96 Aqueous One Solution Reagent Results FACS results showed that the positive rates of HOS CR2 and HOS CD4CR2 cells were greater than 96%, and Western blot showed that the CR2 expression levels on HOS CR2 and HOS CD4CR2 cells were high Activation and blocking tests of MAPKs (ERK, JNK, and p38MAPK) were carried out in HOS CR2, HOS CD4, and HOS CD4CR2 cells The activation of MAPKs in HOS CR2 cells stimulated with PMA (100 ng/ml) and NHS (10%) was identical The activation of MAPKs increased at 5 minutes, reached a peak at 10 minutes, and decreased to baseline within 30 minutes, all in a time dependent manner; the activation of MAPKs was blocked by anti CR2 McAb, PD98059 (inhibitor of ERK), and Wortmanin (inhibitor of PI 3K), respectively In HOS CD4 cells, MAPKs were activated by HIV gp160 In HOS CD4CR2 cells, MAPK activation was induced by HIV gp160, 10% NHS, and HIV gp160+10%NHS; phosphorylation of p38MAPK was dramatically induced by HIV gp160+NHS, and lasted for 1 hour The cell proliferation results showed that HIV gp160 inhibited the proliferation of HOS CD4 and HOS CD4CR2 cells ( P <0 01) and that NHS enhanced the effect of HIV gp160 ( P <0 01) Conclusions The activation of MAPKs is independently mediated by CR2 and that anti CR2 McAb, PD98059, and Wortmanin block the activation of MAPKs, respectively The results of the signal transduction and cell proliferation assays of HOS CD4CR2 cells show that CR2 plays a role in the pathogenesis of HIV infection, especially in the inhibition of CD 4 + cell | 罗敏华 陈明亮 Heribert Stoiber Manfred P Dierich | 2004 | Chinese Medical Journal2004，17，12： | 2 |
| 2 | B cell-mediated infection of stimulated and unstimulated autologous T lymphocytes with HIV-1 : role of complement显示文摘 | Doepper S Stoiber H Kacani L | 2000 | Irnmunobiology2000，202，3： | 1 |
| 3 | Lipopolysaccharide induces in macrophages the synthesis of the suppressor of cytokine signaling 3 and suppresses signal transduction in response to the activating factor IFN-gamma显示文摘 | Stoiber D Kovarik P Cohney S | 1999 | J Immunol1999，163，5： | 1 |
| 4 | Minimal residual disease in ovarian cancer as a target for complement-mediated mAb immunotherapy显示文摘 | BJORGE L STOIBER H DIERICH M P | 2006 | Scand J Immuno2006，63，5： | 1 |
| 5 | Biomechanical properties of decellularized porcine pulmonary valve conduits显示文摘 | Seebacher G Grasl C Stoiber M | | 0，，01： | 1 |
| 6 | One stage uncemented revision of infected total hip replacement using cancellous allograft bone impregnated with antibiotics显示文摘 | Winkler H Stoiber A Kaudela K | 2008 | J Bone Joint Surg Br2008，90，12： | 1 |
| 7 | Histopathology of human corneas after amniotic membrane and limbal stem cell transplantation for severe chemical burn显示文摘 | Stoiber J Muss WH Pohla-Gubo G | 2002 | Cornea2002，21，： | 1 |
| 8 | Complement in different stages of HIV infection and pathogenesis 显示文摘 | Speth C Stoiber H Dierich M P | 2003 | Int Arch Allergy Immunol2003，130，4： | 1 |
| 9 | Fatigue properties of Ti-based hard coatings deposited onto tool steels显示文摘 | Stoiber M Panzenbck M Mitterer C | 2001 | Surface and Coatings Technology2001，，142144： | 1 |
| 10 | Interaction of metal saltswith cytoskeletal motor protein systems 显示文摘 | Their R Bonacker D Stoiber T | 2003 | Toxicology letters2003，140，： | 1 |
| 11 | Epstein-barr virus-induced expression of a novel human vault RNA显示文摘 | Nandy C Mrazek J Stoiber H | 2009 | J Mol Biol2009，388，4： | 1 |
| 12 | Role of complement in the control of HIV dynamics and pathogenesis 显示文摘 | Stoiber H Speth C Dierich MP | 2003 | Vaccine2003，212，： | 1 |
| 13 | Genotoxicity of inorganic lead salts and disturbance of miemtubule function 显示文摘 | BONACKER D STOIBER T BOHM K J | 2005 | Environ Mol Mutagen2005，45，4： | 1 |
| 14 | Bone resorption at the entry of osseointegrated implants on biomechanical phenomenon:finite element study显示文摘 | Mailat h G Stoiber B Atzek G | 1989 | Stomatol1989，，： | 1 |
| 15 | Metronomic cyelophospha- mide treatment in metastasized breast cancer patients : immunologi- cal effects and clinical outcome显示文摘 | Ge Y Domschke C Stoiber N | 2012 | Cancer Immuanl immunother2012，61，3： | 1 |
| 16 | Glioblastoma multiforme: enhancing survival and quality of life 显示文摘 | Davis ME Stoiber AM | 2011 | Clin J Oncol Nurs2011，15，3： | 1 |
| 17 | Amniotic membrane transplantation with limbal stem cell transplantation as a combined procedure for corneal surface reconstruction 显示文摘 | Stoiber J Ruckhofer J | 2002 | Ophthalmology2002，99，17： | 1 |
| 18 | High damping capacity of shape memory alloys 显示文摘 | Stoiber J Delaey L | 1995 | Zeitschrift fuer Metallkunde1995，86，3： | 1 |
| 19 | Interaction of metalsalts with cyctoskeletal motor protein systems 显示文摘 | Their R Bonacker D Stoiber T | 2003 | Toxicol Letters2003，14，： | 1 |
| 20 | Tyrosine kinase 2 (TYK2) in cytokine signalling and host immunity 显示文摘 | Strobl B Stoiber D Sexl V | 2011 | Frontiers in bioscience: a journal and virtual library2011，16，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员