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| 1 | Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy显示文摘AIM:To study the effect of mechanical stress on the cytoskeleton in lens epithelial cells following conventional phacoemulsification surgery(CPS)and femtosecond laserassisted cataract surgery(FLACS).METHODS:The cytoskeleton of the epithelial cells of the anterior lens capsules(ALC)removed by CPS and FLACS was examined by immunohistochemistry.Expression of the intermediate filament,glial fibrillary acidic protein(GFAP),and glutamine synthetase(GS)immunoreactivity were detected.In order to map the actin network of cells,fluorescently labeled phalloidin was used.The samples were examined using confocal laser scanning microscopy.RESULTS:GFAP expression was visible in a larger number of the epithelial cells after CPS compared to FLACS.In CPS sample’s epithelial cells,GFAP immunoreactivity indicated robust morphological change.Regarding the actin filaments,the presence of tubular elements connecting epithelial cells,regular actin pattern and marked cortical network after CPS were found.Following FLACS,the actin cytoskeleton of the epithelial cells remained densely structured,and the tubular elements were undetectable,however,the above-mentioned regular actin pattern and the marked cortical network were visible.CONCLUSION:The conventional removal of the ALC induces more robust changes of the cytoskeleton of the lens epithelial cells. | ANDrea Krisztina Sükosd Krisztina Szabadfi Edina Szabó-Meleg Beáta Gáspár Pavel Stodulka Gyorgy SétálóJr Róbert Gábriel Miklós Nyitrai Zsolt Biró Hajnalka Abrahám | 2020 | International Journal of Ophthalmology(English edition)2020，13，6： | 2 |
| 2 | Efficacy, safety, and flap dimensions of a new femtosecond laser for laser in situ keratomileusis 显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 3 | Efficacy, Safety, and flap dimensions of a new femtosecond laser for laser in situ ker- atomileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 4 | Efficacy, safety, and flap dimensions of a new femtosecond laser for laser in situ keratomileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 5 | Preparation of benzalkonium salts differing in the length of a side alkyl chain 显示文摘 | Kuca K Marek J Stodulka P | 2007 | Mole- cules2007，12，10： | 1 |
| 6 | Efficacy,safety,and flap dimensions of a new femtosecond laser for laser in situ keratom- ileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 7 | Efficacy,safety,and flap dimensions of a new femtosecond laser for laser in situ keratomileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 8 | Efficacy,safety,and flap dimensions of a new femtosecond laser for laser in situ keratomileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | | 0，，： | 1 |
| 9 | In vitro screening of blood-brain barrier penetration of clinically used acetylcholinesterase rcactiva- tors显示文摘 | Karasova JZ Stodulka Kuea K | 2010 | J Appl Biomed2010，8，1： | 1 |
| 10 | Efficacy, safety, and flap dimensions of a new femtosecond laser for laser in situ keratomileusis 显示文摘 | Vryghem J C Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 11 | Efficacy, safety, and flap dimensions of a new femtosecond laser for laser in situ keratomileusis 显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，3： | 1 |
| 12 | Efficacy,safety,and flap dimensions of a new femtosecond laser for laser in situ keratomileusis显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，： | 1 |
| 13 | Efficacy, safety,and flap dimensions of a new femtosecond laser for laser in si- tu keratomileusis 显示文摘 | Vryghem JC Devogelaere T Stodulka P | 2010 | J Cataract Refract Surg2010，36，： | 1 |
| 14 | Analytical and Numerical Modifications of Transonic Nozzle Flows显示文摘Classical transonic hodograph-based design methods are employed and revitalized using modern numerical tools to illustrate the design of a symmetrical accelerating-decelerating nozzle throat design. The concept of Elliptic Continuation is applied to solve transonic boundary value problems avoiding the inherently nonlinear nature of the basic equations and obtaining transonic flow examples using the Method of Characteristics in an inverse mode. Purpose of the present paper, besides describing a new special flow example, is to keep these classical methods alive for education of a new generation of creative engineers. | Jiri STODULKA Helmut SOBIECZKY Pavel SAFARIK | 2018 | Journal of Thermal Science2018，27，4： | 0 |
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