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| 1 | 直刺变豆菜叶绿体全基因组及其特征显示文摘直刺变豆菜(Sanicula orthacantha)是中国广泛分布的多年生草本植物,也是一味著名的民族药。本文通过二代高通量测序平台Illumina HiSeq PE150对直刺变豆菜叶绿体全基因组进行测序,并通过生物信息学方法对其结构特征进行分析。结果表明:直刺变豆菜叶绿体全基因组大小为157,163bp,包括大单拷贝区(largesinglecopy,LSC)、小单拷贝区(smallsinglecopy,SSC)和2个反向重复序列(invertedrepeatsequence,IRa和IRb),长度分别为87,547 bp、17,122 bp和26,247 bp,具有典型被子植物叶绿体基因组环状四分体结构;共注释得到129个基因,包括8个核糖体RNA (rRNA)基因、37个转运RNA (tRNA)基因和84个蛋白质编码基因。直刺变豆菜在叶绿体基因组结构、基因种类、排列顺序上与其他伞形科植物基本一致。直刺变豆菜叶绿体全基因组测序的成功为变豆菜属植物完整叶绿体基因组组装及其特征分析提供了新的方法。 | 陈志祥 姚雪莹 Stephen R.Downie 王奇志 | 2019 | 生物多样性2019，27，4： | 4 |
| 2 | Historical biogeography of the Angelica group (Apiaceae tribe Selineae) inferred from analyses of nrDNA and cpDNA sequences显示文摘Biogeographical 模式和以亚洲为中心的被子植物组的多样化过程显著地受影响了多级式后来 HimalayasTibetan 高原高举晚第三级。分叉时间大部分东方亚洲当归组(Apiaceae,亚科 Apioideae,部落 Selineae ) 开始用野兽和 nrDNA 被分析从部落 Selineae 和亲戚的 96 个代表的内部抄录分隔符顺序数据。当归组的 biogeographical 历史的进一步的分析在包含所有下列 loci 或一些的数据集上用野兽, S 歌剧女主角,粗锉,和 Lagrange 被执行:内部、外部的 nrDNA 抄录了分隔符;cpDNA rps16 intron;并且 cpDNA rps16-trnK, rpl32-trnL,和 trnL-trnT intergenic 分隔符。结果建议当归组原来在迟了的中间的全球冷却期间是在东方 Palearctic 的现在中新世(13.6 Mya ) 并且当归 s.s clade 在 10.2 Mya 在一样的区域发源。当归 s.s clade 的随后的多样化在中间期间在东方 Palearctic 加强了晚中新世(10.07.0 Mya ) 并且在东方喜玛拉雅的地区在迟了的上新世和更新世期间(< 4.0 Mya ) 。这些多样化多半与高原高举驾驶相应气候的变化。就 elevational 重建而言,对高度的微分回答看起来是在东方喜马拉雅山脉解释这个组的最近的放射的主要因素。当归组的北美的种类作为 polyphyletic 被检索,至少自从中间,他们的迁居包含了六独立疏开到北美洲晚中新世,包括从欧洲的从东北亚洲并且两次的四次。 | Chen-Yang LIAO Stephen R.DOWNIE Yan YU Xing-Jin HE | 2012 | Journal of Systematics and Evolution2012，50，3： | 4 |
| 3 | 变豆菜属15种植物的果实微形态特征及其分类学意义显示文摘采用扫描电镜对来自北美和东亚的变豆菜属植物(美国7个种,中国8个种)的果实表面微形态进行观察,结合前人对伞形科其他类群果实微形态特征的研究及近年来分子系统学证据,对变豆菜属的种间分类进行研究。结果显示,变豆菜属植物果实的形态大小、皮刺弯曲程度和果柄蜡质纹饰丰富多样。研究表明变豆菜属是一个自然类群,果实表面钩刺结构有利于该属植物的传播及扩散,果实微形态特征具有分类学价值,可作为种间分类的依据。根据变豆菜属果实表面微形态特征,编制了该属植物分种检索表。 | 陈志祥 姚雪莹 Stephen R.Downie 王奇志 | 2019 | 植物科学学报2019，37，1： | 3 |
| 4 | A systematic study of North American Angelica species(Apiaceae)based on nrDNA ITS and cpDNA sequences and fruit morphology显示文摘Angelica is a taxonomically complex genus widespread throughout the North Temperate Zone.Previous phylogenetic studies of the genus have focused primarily on its East Asian species.The relationships among its North American members,the monophyly of these species,and the value of fruit morphology in circumscribing its taxa have yet to be examined.This study represents the most comprehensive sampling of Angelica to date(100 species)and includes all 26 species in North America.Relationships are inferred using Bayesian inference,maximum likelihood,and maximum parsimony analyses of ITS sequences and,for multiple accessions of each North American species,cpDNA ndhF-rpl32,rpl32-trnL,and psbM-psbD sequences.The fruit morphological characters examined were those considered phylogenetically important in East Asian Angelica.The results revealed that the North American species fell into three major clades:North American Angelica clade,Archangelica clade,and the Eurasian Angelica clade.Angelica dawsonii has affinities with Lomatium brandegeei.Fourteen species within the North American Angelica clade were strongly supported as monophyletic.Two paraphyletic species resulted in new combinations in A.lineariloba and A.venenosa.Conflict between the ITS-derived and cpDNA-derived phylogenies and the lack of resolution in portions of the trees may be due to chloroplast capture and rapid species radiation.Fruit morphology supported some interspecific relationships based on molecular data,and relationships revealed by ITS and cpDNA data were roughly in accordance with fruit classification type and geographic distribution region,respectively.A diagnostic key based on fruit morphology is provided for the identification of the North American Angelica taxa. | Chen-Yang Liao Qing Gao Deborah S.Katz-Downie Stephen R.Downie | 2022 | Journal of Systematics and Evolution2022，60，4： | 1 |
| 5 | Circumscription of the East Asia clade (Apiaceae subfamily Apioideae) and the taxonomic placements of several problematic genera显示文摘The East Asia(or Physospermopsis) clade was recognized in previous molecular phylogenetic investigations into the higher-level relationships of Apiaceae subfamily Apioideae. The composition of this clade, the phylogenetic relationships among its constituent taxa, and the placement of species previously determined to be problematic have yet to be resolved. Herein, nr DNA ITS sequences were obtained for150 accessions of Apioideae, representing species whose distributions are in East Asia or genera having one or more species included within the East Asia clade. These data, along with published ITS sequences from other Apioideae(for 3678 accessions altogether), were subjected to maximum likelihood and Bayesian inference analyses. The results show that the East Asia clade contains representatives of 11currently recognized genera: Hansenia, Hymenolaena, Keraymonia, Sinolimprichtia, Acronema, Hymenidium, Physospermopsis, Pimpinella, Sinocarum, Tongoloa, and Trachydium. However, the latter seven genera have members falling outside of the East Asia clade, including the generic types of all except Tongoloa. Within the clade, the species comprising these seven genera are widely intermingled, greatly increasing confusion among relationships than previously realized. The problematic species Physospermopsis cuneata is confirmed as falling within the East Asia clade, whereas P. rubrinervis allies with the generic type in tribe Pleurospermeae. Physospermopsis kingdon-wardii is confirmed as a member of the genus Physospermopsis, whereas the generic attributions of P. cuneata and Tongoloa stewardii remain unclear. Two species of Sinocarum(S. filicinum and S. wolffianum) are transferred into the genus Meeboldia. This is the most comprehensive molecular phylogenetic investigation of the East Asia clade to date, and while the results increase systematic understanding of the clade, they also highlight the need for further studies of one of the most taxonomically intractable groups in Apioideae. | Jing Zhou Xinyue Wang Shilin Zhou Junmei Niu Jiarui Yue Zhenwen Liu Stephen R.Downie | 2024 | Plant Diversity2024，46，2： | 0 |
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