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| 1 | Engineering metabolic pathways in Amycolatopsis japonicum for the optimization of the precursor supply for heterologous brasilicardin congeners production显示文摘The isoprenoid brasilicardin A is a promising immunosuppressant compound with a unique mode of action,high potency and reduced toxicity compared to today's standard drugs.However,production of brasilicardin has been hampered since the producer strain Nocardia terpenica IFM0406 synthesizes brasilicardin in only low amounts and is a biosafety level 2 organism.Previously,we were able to heterologously express the brasilicardin gene cluster in the nocardioform actinomycete Amycolatopsis japonicum.Four brasilicardin congeners,intermediates of the BraA biosynthesis,were produced.Since chemical synthesis of the brasilicardin core structure has remained elusive we intended to produce high amounts of the brasilicardin backbone for semi synthesis and derivatization.Therefore,we used a metabolic engineering approach to increase heterologous production of brasilicardin in A.japonicum.Simultaneous heterologous expression of genes encoding the MVA pathway and expression of diterpenoid specific prenyltransferases were used to increase the provision of the isoprenoid precursor isopentenyl diphosphate(IPP)and to channel the precursor into the direction of diterpenoid biosynthesis.Both approaches contributed to an elevated heterologous production of the brasilicardin backbone,which can now be used as a starting point for semi synthesis of new brasilicardin congeners with better properties. | Paul N.Schwarz Luisa Roller Andreas Kulik Wolfgang Wohlleben Evi Stegmann | 2018 | Synthetic and Systems Biotechnology2018，3，1： | 3 |
| 2 | The presence of immune stimulatory cells in fresh and cryopreserved donor aortic and pulmonary valve allografts显示文摘 | Stegmann AP van der Ham F | 2002 | J Heart Valve Dis2002，11，3： | 1 |
| 3 | Knowledge conver- gence in collaborative learning: Concepts and assessment显示文摘 | Weinberger A Stegmann K Fischer F | 2007 | Learning and Instruction2007，17，4： | 1 |
| 4 | Viscocanalostomy for open-angle glaucoma in black African patients显示文摘 | Stegmann R Pienagr A Miller D | 1999 | J Cataract Refract Surg1999，25，3： | 1 |
| 5 | The virosome concept for influenza vaecines显示文摘 | Huckriede A Bungener L Stegmann T | 2005 | Vaccine2005，8，23： | 1 |
| 6 | Differential radioactive quantification of protein abundance ratios between benign and malignant prostate tissues: cancer association of annexin A3显示文摘 | Wozny W Schroer K Schwall G P Poznanovic S Stegmann W Dietz K | 2007 | Proteomics2007，7，： | 1 |
| 7 | Lac- tate kinetics and individual anaerobic threshold 显示文摘 | Stegmann H W Kindermann W Schnabela A | 1981 | Int J Sports Med1981，2，3： | 1 |
| 8 | Toxoplasmosis,other(syphilis,varicella-zoster,parvovirus B19),rubella,cytomegalovirus(CMV),and herpes infections显示文摘 | Stegmann BJ Carey JC | 2002 | Curr Womens Health Rep2002，2，4： | 1 |
| 9 | Clinical experience with a new collagen-coated Dacron double-velour prosthesis显示文摘 | Stegmann T Haverich A Borst HG | 1986 | Thorac Cardiovasc Surg1986，34，： | 1 |
| 10 | Viscocanalostony for open-angle glaucoma in black african patients显示文摘 | Stegmann R Pienaar A Miller D | 1999 | J Cataract Refract Surg1999，25，3： | 1 |
| 11 | A new approach to ovarian reserve, testing 显示文摘 | Sun W Stegmann BJ Henne MA | 2008 | Fertil Steri12008，90，6： | 1 |
| 12 | 动物通讯理论显示文摘通讯行为见于所有的动物分类阶元;不同的动物感觉系统不同,所用通讯手段也各异。低等动物主要采用化学和触觉信号;鱼类采用视觉和嗅觉信号;鸟类更多地用听觉和视觉信号;哺乳动物应用所有的感觉通道。通讯见于同种动物之间,也见于异种动物之间。最高级的通讯形式是语言。语言能力是灵长类动物才具有的,和用火、制造工具一起促进了人类的演化。 | Ulrich E. Stegmann 魏玉保 | 2014 | 国外科技新书评介2014，，5： | 1 |
| 13 | Hypothesis Generation Guided by Co-word Clustering显示文摘 | J Stegmann Guenter G | 2003 | Scientometrics2003，56，1： | 1 |
| 14 | Characterization of barrier/seed layer stacks of Cu interconnects by electron tomographic three-dimensional object reconstruction显示文摘 | Engelmann H Zschech E | 2003 | Microelectronic Engineering2003，65，： | 1 |
| 15 | Paradoxical reactions to rectal midazolam as premedication in children显示文摘 | Roelofse JA Stegmann DH Hartsbome J | 1990 | Int J Oral Maxillofac Surg1990，19，1： | 1 |
| 16 | Hypothesis Generation Guided by Co-word Clustering显示文摘 | STEGMANN Johannes GROHMANN Guenter | 2003 | Scientometrics2003，56，1： | 1 |
| 17 | Interferon-alpha-induced TRAIL on natural killer cells is associated with control of hepatitis C virus infection显示文摘 | Stegmann KA BjOrkstrOm NK Veber H | 2010 | Gastroenterology2010，138，5： | 1 |
| 18 | Radiative forcing due to anthropogenic aerosols over the Asian pacific region显示文摘 | Sokolik I N Stegmann P M | 1999 | Journal of Geophysical Research1999，30，： | 1 |
| 19 | Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats 显示文摘 | Kamphuis T Shafique M Meijerhof T Stegmann T Wilschut J de Haan A | 2013 | Vaccine2013，31，17： | 1 |
| 20 | CO2 Corrosion and Its Prevention by Chemical Inhibitionin in Oil and Gas Production 显示文摘 | HAUSLER R H STEGMANN D W | 1988 | Corrosion1988，44，1： | 1 |
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