| 11 | Influence of fibre and betaine on development of the gastrointestinal tract of broilers between hatch and 14 d of age显示文摘An experiment was designed to determine the influence of fibre and betaine on the development of the intestine, liver and pancreas of broilers from hatch to 14 d of age. A total of 250-day-old Cobb 500 male broilers were allocated to 16 cages with 15 broilers each. Treatments were arranged in a 2 x 4 factorial design, consisting of 2 feed formulations(low and high fibre diets) and 4 levels of betaine(0,1, 3 or 5 kg/t). At hatch, 10 birds in total were euthanised, and samples of the liver, pancreas, yolk sac and intestine were collected for reference of the analysed parameters before the start of the trial.On d 4.9 and 14, 5 birds per cage(10 birds per treatment) were selected, euthanised and treated as the same as the birds at hatch. Villus height and width and crypt depth were determined on the duodenum samples, and absorptive area was calculated. The number of enterocytes in mitosis at the villus was determined by a positive reaction to antibody for Ki67 protein, and fused villus was evaluated visually. The relative weight of the yolk sac reduced(P < 0.05) as birds aged while the intestine and liver reached a maximum(P < 0.05) at around d 4 and the pancreas at d 9. Birds fed the high fibre diet had greater feed intake, lower relative weight of the pancreas and higher villus(P < 0.05) than birds fed the low fibre diet. Villus width increased(P < 0.05) at 4 d of age, and this was associated with fused villus. Betaine inclusion reduced(P < 0.05) villus width, increased(P < 0.05) villus size and absorptive area, and reduced(P < 0.05) the number of enterocytes with positive reaction for the antibody Ki-67.Betaine inclusion reduced the width and increased the absorptive area and the villus height of the duodenum of birds up to 14 d of age. The higher fibre diet increased feed intake and villus height, yet reduced pancreas relative weight, while not affecting body weight gain. This response was possibly due to a dilution effect of the fibre, reducing nutrient absorption and consequently stimulating villus growth to improve absorption rates. | Tiago T.dos Santos Sthéfanie C.Dassi Celia R.C.Franco Cleber R.V.da Costa Sophie A.Lee Ana V.Fisher da Silva | 2019 | Animal Nutrition2019，，2： | 0 |
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