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| 1 | Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability,migration and proliferation in vitro显示文摘AIM: To analyze the concentration-dependent effects of autologous serum(AS) and fetal bovine serum(FBS) on human corneal epithelial cell(HCEC) viability, migration and proliferation.METHODS: AS was prepared from 13 patients with nonhealing epithelial defects Dulbecco's modified eagle medium/Ham's F12(DMEM/F12) with 5% FBS, 0.5% dimethyl sulphoxide(DMSO), 10 ng/m L human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS. HCEC viability was analyzed using cell proliferation kit XTT, migration using a wound healing assay, proliferation by the cell proliferation enzyme-linked immunosorbent assay(ELISA) Brd U kit. Statistical analysis was performed using the generalized linear model, the values at 30% AS or 30% FBS were used as the baselines.RESULTS: HCEC viability was the highest at 30% AS or 15% FBS and the lowest at 10% AS or 30% FBS application. HCEC migration was the quickest through 30% AS or 30% FBS and the slowest through 5% AS or 5% FBS concentrations. Proliferation was the most increased through 15% AS or 5% FBS and the least increased through 30% AS or 30% FBS concentrations. HCEC viability at 10% and 15% AS was significantly worse(P=0.001, P=0.023) compared to baseline and significantly better at 15% FBS(P=0.003) concentrations. HCEC migration was significantly worse(P≤0.007) and HCEC proliferation significantly better(P<0.001) in all concentration groups compared to baseline.CONCLUSION: For the best viability of HCEC 30% AS or 15% FBS, for HCEC migration 30% AS or 30% FBS, for proliferation 15% AS or 5% FBS should be used. Therefore, we suggest the use of 30% AS in clinical practice. | Ming-Feng Wu Tanja Stachon Berthold Seitz Achim Langenbucher Nóra Szentmáry | 2017 | International Journal of Ophthalmology(English edition)2017，10，6： | 7 |
| 2 | Inflammatory mecha-nisms in atherosclerosis 显示文摘 | Wolf D Stachon P Bode C | 2014 | Hamostaseologie2014，34，1： | 1 |
| 3 | Catecholaminedependent hereditary Cushing's syndrome-follow-up after unilateral adrenalectomy显示文摘 | Imohl M Koditz R Stachon A | 2002 | Med Klin (Munich)2002，97，： | 1 |
| 4 | The secretion of endothelin-1 by microvascular endothelial cells from human benign prostatic hyperplasia is inhibited by vascular endothelial growth factor 显示文摘 | Stachon A Schluter T Junker K | 2004 | Growth Factors2004，22，4： | 1 |
| 5 | TRAF5 deficiency accelerates atherogenesis in mice by increasing inflammatory cell recruitment and foam cell formation显示文摘 | Missiou A Rudolf P Stachon P | 2010 | Circ Res2010，107，6： | 1 |
| 6 | Secretion of soluble VEGF receptor 2 by microvascu lar endothelial cells derived by human benign prostatic hyperplasia显示文摘 | STACHON A AWEIMER A STACHON T | 2009 | Growth Factors2009，27，： | 1 |
| 7 | Prognostic significance of low serum cholesterol after cardiothoracic surgery显示文摘 | Stachon A Boning A Weisser H | 2000 | Clin Chem2000，46，81： | 1 |
| 8 | A laboratory-based risk score for medical intensive care patients显示文摘 | Stachon A Segbers E Hering S | 2008 | Clin Chem Lab Med2008，46，6： | 1 |
| 9 | A laboratory-based risk score for medical intensive care patients显示文摘 | Stachon A Segbers E Hering S | | 0，，6： | 1 |
| 10 | Secretion of soluble VEGF receptor 2 by microvascular endothelial cells deriverd by human benign prostate hyperplasia显示文摘 | Stachon A Aweimer A Stachin T | 2009 | Growth Factors2009，27，： | 1 |
| 11 | Population distribution modelling at fine spatio-temporal scale based on mobile phone data显示文摘Population distribution modelling can benefit many different domains,for example,transportation,urban planning,ecology or emergency management.Information about the location and number of people in an affected area is crucial for decision-makers during emergencies and crises.Mobile phone data represents relatively reliable and time accurate information on real-time population distribution,movement and behaviour.In this study,we evaluate the spatio-temporal distribution of population derived from phone data of the selected pilot area(City of Brno,Czech Republic).Analysis is based on the dataset describing the estimated human presence(EHP)with two values-visitors and transiting persons.The temporal change of data is first analysed and further processed using two methodological approaches.First,the dasymetric method is used where the building geometry and technical attributes served as a target layer.Second,the results of building level analysis are transformed into a regular grid zone of both visitors and the general EHP.Resulting spatio-temporal patterns are compared to the census data.Results demonstrate how the proposed building level dasymetric approach can improve the spatial granularity of EHP.Potential use of proposed methodology within selected emergency situations is further discussed. | Petr Kubicek Milan Konecny Zdenek Stachon Jie Shen Lukas Herman Tomas Reznik Karel Stanek Radim Stampach Simon Leitgeb | 2019 | International Journal of Digital Earth2019，12，11： | 1 |
| 12 | A laboratory-based risk scorefor medical intensive care patients显示文摘 | Stachon A Segbers E Hering S | 2008 | Clin Chem Lab Med2008，46，6： | 1 |
| 13 | Gentamiein treatment in McArdle disease:failure to correct myophosphorylase deficiency显示文摘 | SCHROERS A KLEY RA STACHON A | 2006 | Neurology2006，66，： | 1 |
| 14 | High in-hospital mortality of intensive care patients with hueleated red blood cells in blood显示文摘 | Stachon A Holland-Letz T Krieg M | 2004 | Clin chem Lad Med2004，42，8： | 1 |
| 15 | Inflammatory mechanisms in atherosclerosis 显示文摘 | Wolf D Stachon P Bode C | 2014 | Hamostaseologie2014，34，1： | 1 |
| 16 | Regu lation of soluble VEGFR-2 secreted by microvascular endothelial ceils derived from human BPH显示文摘 | AWEIMER A STACHON T TANNAPFEL A | 2012 | Prostate Cancer Prostatic Dis2012，15，2： | 1 |
| 17 | Nucleated red blood cells indicate high risk of in- hospital mortality 显示文摘 | STACHON A SONDERMANN N IMOHL M | 2002 | J Lab Clin Med2002，140，6： | 1 |
| 18 | High in-hospital mortality of intensive care patients with nucleated red blood cells in blood显示文摘 | STACHON A HOLLAND-LETZ T KRIEG M | 2004 | Clin Chem Lab Med2004，42，8： | 1 |
| 19 | Primary culture of microvascular endothelial cells from human benign prostatic hyperplasia显示文摘 | Stachon A Schluter T Koller M | 2001 | Prostate2001，48，3： | 1 |
| 20 | The secretion of endothelin-1 by microvascular endothelial cells from human benign prostatic hyperplasia is inhibited by vascular endothelial growth factor显示文摘 | Schluter T Junker K | 2004 | Growth Factors2004，22，： | 1 |
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