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| 1 | Adiponectin affects the mechanical responses in strips from the mouse gastric fundus显示文摘AIM To investigate whether the adipocytes derived hormone adiponectin(ADPN) affects the mechanical responses in strips from the mouse gastric fundus. METHODS For functional experiments, gastric strips from the fundal region were cut in the direction of the longitudinal muscle layer and placed in organ baths containing KrebsHenseleit solution. Mechanical responses were recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrical field stimulation(EFS) was applied via two platinum wire rings through which the preparation was threaded. The effects of ADPN were investigated on the neurally-induced contractile and relaxant responses elicited by EFS. The expression of ADPN receptors, Adipo-R1 and Adipo-R2, was also evaluated by touchdown-PCR analysis. RESULTS In the functional experiments, EFS(4-16 Hz) elicited tetrodotoxin(TTX)-sensitive contractile responses. Addition of ADPN to the bath medium caused a reduction in amplitude of the neurally-induced contractile responses(P < 0.05). The effects of ADPN were no longer observed in the presence of the nitric oxide(NO) synthesis inhibitor L-NG-nitro arginine(L-NNA)(P > 0.05). The direct smooth muscle response to methacholine was not influenced by ADPN(P > 0.05). In carbachol precontracted strips and in the presence of guanethidine, EFS induced relaxant responses. Addition of ADPN to the bath medium, other than causing a slight and progressive decay of the basal tension, increased the amplitude of the neurally-induced relaxant responses(P < 0.05). Touchdown-PCR analysis revealed the expression of both Adipo-R1 and Adipo-R2 in the gastric fundus.CONCLUSION The results indicate for the first time that ADPN is able to influence the mechanical responses in strips from the mouse gastric fundus. | Eglantina Idrizaj Rachele Garella Giovanni Castellini Hermine Mohr Natalia S Pellegata Fabio Francini Valdo Ricca Roberta Squecco Maria Caterina Baccari | 2018 | World Journal of Gastroenterology2018，24，35： | 2 |
| 2 | Effects of sphingosine 1-phosphate on excitation-contraction coupling in mammaliea skeletal muscle显示文摘 | Bendni C Squecco R Piperio C | 2003 | Muscle Res Cell Motil2003，，： | 1 |
| 3 | Sphingosine 1- phosphate induces differentiation of adipose tissue- derived mesenchymal stem cells towards smooth muscle cells 显示文摘 | Nincheri P Luciani P Squecco R | 2009 | Cell Mol Life Sci2009，，10： | 1 |
| 4 | Charaterization of human adult stem-cellpopulations isolated from visceral and subcutaneous adipose tissue显示文摘 | Baglioni S Francalanci M Squecco R | | 0，，： | 1 |
| 5 | Sphingosine l-phosphate induces myoblast differentiation through Cx43 protein expression : a role for a gap junction-depend- ent and-independent function显示文摘 | Squecco R Sassoli C Nuti F Martinesi M Chellini F Nosi D | 2006 | Mol Biol Cell2006，17，11： | 1 |
| 6 | Regulation of transient receptor potential canonical channel 1 (TRPC1) by sphingosine 1-phosphate in C2C12 myoblasts and its relevance for a role of mechanotransduction in skeletal muscle differentiation 显示文摘 | Formigli L Sassoli C Squecco R | 2009 | J Cell Sci2009，122，9： | 1 |
| 7 | Characterization of human adult stem-cel populations isolated from visceral and subcutaneous adipose tissue显示文摘 | Baglioni S Francalanci M Squecco R | | 0，，10： | 1 |
| 8 | The vitamin D receptor agonist elocalcitol upregulates L-type calcium channel activity in human and rat bladder显示文摘 | Morelli A Squecco R Failli P | | 0，，05： | 1 |
| 9 | Glucagon-like peptide-2 modulates the nitrergic neurotransmission in strips from the mouse gastric fundus显示文摘AIM To investigate whether glucagon-like peptide-2(GLP-2) influences the neurally-induced responses in gastric strips from mice, since no data are available. METHODS For functional experiments, gastric fundal strips were mounted in organ baths containing Krebs-Henseleit solution. Mechanical responses were recorded via forcedisplacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrical field stimulation(EFS) was applied via two platinum wire rings through which the preparationwas threaded. The effects of GLP-2(2 and 20 nmol/L) were evaluated on the neurally-induced contractile and relaxant responses elicited by EFS. Neuronal nitric oxide synthase(n NOS) enzyme was evaluated by immunohistochemistry.RESULTS In the functional experiments, electrical field stimulation(EFS, 4-16 Hz) induced tetrodotoxin(TTX)-sensitive contractile responses, which were reduced in amplitude by GLP-2(P < 0.05). In the presence of the nitric oxide(NO) synthesis inhibitor L-NNA, GLP-2 no longer influenced the neurally-evoked contractile responses(P > 0.05). The direct smooth muscle response to methacholine was not influenced by GLP-2(P > 0.05). In the presence of guanethidine and carbachol, the addition of GLP-2 to the bath medium evoked TTX-sensitive relaxant responses that were unaffected by L-NNA(P > 0.05). EFS induced a fast NO-mediated relaxation, whose amplitude was enhanced in the presence of the hormone(P < 0.05). Immunohistochemical experiments showed a significant increase(P < 0.05) in n NOS immunoreactivity in the nerve structures after GLP-2 exposure. CONCLUSION The results demonstrate that in gastric fundal strips, GLP-2 influences the amplitude of neurally-induced responses through the modulation of the nitrergic neurotransmission and increases n NOS expression. | Rachele Garella Eglantina Idrizaj Chiara Traini Roberta Squecco Maria Giuliana Vannucchi Maria Caterina Baccari | 2017 | World Journal of Gastroenterology2017，23，40： | 0 |
| 10 | Can adiponectin have an additional effect on the regulation of food intake by inducing gastric motor changes?显示文摘The regulation of food intake is a complex mechanism,and the hypothalamus is the main central structure implicated.In particular,the arcuate nucleus appears to be the most critical area in the integration of multiple peripheral signals.Among these signals,those originating from the white adipose tissue and the gastrointestinal tract are known to be involved in the regulation of food intake.The present paper focuses on adiponectin,an adipokine secreted by white adipose tissue,which is reported to have a role in the control of feeding by acting centrally.The recent observation that adiponectin is also able to influence gastric motility raises the question of whether this action represents an additional peripheral mechanism that concurs with the central effects of the hormone on food intake.This possibility,which represents an emerging aspect correlating the central and peripheral effects of adiponectin in the hunger-satiety cycle,is discussed in the present paper. | Eglantina Idrizaj Rachele Garella Roberta Squecco Maria Caterina Baccari | 2020 | World Journal of Gastroenterology2020，26，20： | 0 |
| 11 | Relaxin influences ileal muscular activity through a dual signaling pathway in mice显示文摘AIM To investigate the signaling pathways involved in the relaxin(RLX) effects on ileal preparations from mice through mechanical and electrophysiological experiments.METHODS For mechanical experiments, ileal preparations from female mice were mounted in organ baths containing Krebs-Henseleit solution. The mechanical activity was recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrophysiological measurements were performed in current-and voltage-clamp conditions by a microelectrode inserted in a single smooth muscle cell(SMC) of the ileal longitudinal layer. Both the membrane passive properties and inward voltage-dependent L-type Ca2+ currents were recorded using suitable solutions and voltage stimulation protocols.RESULTS Mechanical experiments showed that RLX induced a decay of the basal tension and a reduction in amplitude of the spontaneous contractions. The effects of RLX were partially reduced by 1 H-[1,2,4]oxadiazolo[4,3-a ]-quinoxalin-1-one(ODQ) or 9-cyclopentyladenine mesylate(9 CPA), inhibitors of guanylate cyclase(GC) and adenylate cyclase(AC), respectively, and were abolished in the concomitant presence of both drugs. Electrophysiological experiments demonstrated that RLX directly influenced the biophysical properties of ileal SMCs, decreasing the membrane conductance, hyperpolarizing the resting membrane potential, reducing the L-type calcium current amplitude and affecting its kinetics. The voltage dependence of the current activation and inactivation time constant was significantly speeded by RLX. Each electrophysiological effect of RLX was reduced by ODQ or 9 CPA, and abolished in the concomitant presence of both drugs as observed in mechanical experiments. CONCLUSION Our new findings demonstrate that RLX influences ileal muscle through a dual mechanism involving both GC and AC. | Eglantina Idrizaj Rachele Garella Fabio Francini Roberta Squecco Maria Caterina Baccari | 2018 | World Journal of Gastroenterology2018，24，8： | 0 |
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