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您的检索式：作者名="Songmei Geng"
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| 1 | Efficient gene transfection of suspension cells by highly branched poly(β-amino ester)显示文摘Suspension cells play a crucial role in many biological processes. However, compared to adherent cells, it is particularly challenging to introduce exogenous genes into suspension cells to regulate their biological functions with non-viral gene vectors, mainly due to the low cellular uptake and endosomal escape of polyplexes. Herein, to improve the interactions of polyplexes with cellular membranes, we design and synthesize highly branched poly(β-amino ester)(HPAE) via an “A2 + B4 + C2” Michael addition strategy.Results show that branching significantly increases DNA condensation of HPAE, cellular uptake and endosomal escape of HPAE/DNA polyplexes. In mast cells(MCs), HPAE exhibits up to 80-fold higher gene transfection efficiency compared to the corresponding linear poly(β-amino ester)(LPAE) and the leading commercial gene transfection reagents PEI25k, jetPEI, and Lipofectamine 3000, without causing obvious cytotoxicity. Our study establishes a reliable non-viral platform for efficient gene transfection of suspension cells. | Delu Che Chenfei Wang Zhili Li Kaixuan Wang Shuaiwei Sun Xinyue Zhang Yi Li Zhengju Chen Lei Guo Yajing Hou Dezhong Zhou Songmei Geng | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
| 2 | Constructing skin-equivalents using hair follicle stem cells显示文摘Objective: To establish the method of constructing skin-equivalents (SE) using hair follicle stem cells(HFSC). Methods: K19 positive cells derived from hair were cultivated using serum-free medium KGM and seeded on dermal equivalents (DE). After the culture between the air-liquid interface for 14 days, SE were harvested and used for evaluation. Results: K19 positive cells chosen as HFSC were located in bulge of out root sheet in hair follicle. Cultivated HFSC could build a fully developed, multi-layered epidermis on the basis of DE, resembling the skin structure. Conclusion: HFSC located in out root sheet can differentiate into keratinocyte in vitro and be used for SE construction. | Songmei Geng Shengshun Tan | 2006 | Journal of Nanjing Medical University2006，20，2： | 0 |
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