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| 1 | Counter-regulatory phosphatases TNAP and NPP1 temporally regulate tooth root cementogenesis显示文摘Cementum is critical for anchoring the insertion of periodontal ligament fibers to the tooth root. Several aspects of cementogenesis remain unclear, including differences between acellular cementum and cellular cementum, and between cementum and bone.Biomineralization is regulated by the ratio of inorganic phosphate(Pi) to mineral inhibitor pyrophosphate(PPi), where local Piand PPi concentrations are controlled by phosphatases including tissue-nonspecific alkaline phosphatase(TNAP) and ectonucleotide pyrophosphatase/phosphodiesterase 1(NPP1). The focus of this study was to define the roles of these phosphatases in cementogenesis. TNAP was associated with earliest cementoblasts near forming acellular and cellular cementum. With loss of TNAP in the Alpl null mouse, acellular cementum was inhibited, while cellular cementum production increased, albeit as hypomineralized cementoid. In contrast, NPP1 was detected in cementoblasts after acellular cementum formation, and at low levels around cellular cementum. Loss of NPP1 in the Enpp1 null mouse increased acellular cementum, with little effect on cellular cementum.Developmental patterns were recapitulated in a mouse model for acellular cementum regeneration, with early TNAP expression and later NPP1 expression. In vitro, cementoblasts expressed Alpl gene/protein early, whereas Enpp1 gene/protein expression was significantly induced only under mineralization conditions. These patterns were confirmed in human teeth, including widespread TNAP, and NPP1 restricted to cementoblasts lining acellular cementum. These studies suggest that early TNAP expression creates a low PPienvironment promoting acellular cementum initiation, while later NPP1 expression increases PPi, restricting acellular cementum apposition. Alterations in PPihave little effect on cellular cementum formation, though matrix mineralization is affected. | Laura E Zweifler Mudita K Patel Francisco H Nociti Jr Helen F Wimer Jose L Milln Martha J Somerman Brian L Foster | 2015 | International Journal of Oral Science2015，7，1： | 5 |
| 2 | Cementomimetics——constructing a cementum-like biomineralized microlayer via amelogenin-derived peptides显示文摘Cementum is the outer-,mineralized-tissue covering the tooth root and an essential part of the system of periodontal tissue that anchors the tooth to the bone. Periodontal disease results from the destructive behavior of the host elicited by an infectious biofilm adhering to the tooth root and left untreated,may lead to tooth loss. We describe a novel protocol for identifying peptide sequences from native proteins with the potential to repair damaged dental tissues by controlling hydroxyapatite biomineralization. Using amelogenin as a case study and a bioinformatics scoring matrix,we identified regions within amelogenin that are shared with a set of hydroxyapatite-binding peptides (HABPs) previously selected by phage display. One 22-amino acid long peptide regions referred to as amelogenin-derived peptide 5 (ADP5) was shown to facilitate cell-free formation of a cementum-like hydroxyapatite mineral layer on demineralized human root dentin that,in turn,supported attachment of periodontal ligament cells in vitro. Our findings have several implications in peptide-assisted mineral formation that mimic biomineralization. By further elaborating the mechanism for protein control over the biomineral formed,we afford new insights into the evolution of protein-mineral interactions. By exploiting small peptide domains of native proteins,our understanding of structure-function relationships of biomineralizing proteins can be extended and these peptides can be utilized to engineer mineral formation. Finally,the cementomimetic layer formed by ADP5 has the potential clinical application to repair diseased root surfaces so as to promote the regeneration of periodontal tissues and thereby reduce the morbidity associated with tooth loss. | Mustafa Gungormus Ersin E Oren Jeremy A Horst Hanson Fong Marketa Hnilova Martha J Somerman Malcolm L Snead Ram Samudrala Candan Tamerler Mehmet Sarikaya | 2012 | International Journal of Oral Science2012，4，2： | 5 |
| 3 | Immobilization of alkaline phosphatase on microporous nanofibrous fibrin scaffolds for bone tissue engineering显示文摘 | OSATHANON T GIACHELLI C M SOMERMAN M J | 2009 | Biomaterials2009，30，27： | 1 |
| 4 | Cell attachment ac- tivity of the 44 kilodalton bone phosphoprotein is not restricted to bone cells显示文摘 | Somerman M J Prince CW Butler WT | 1989 | Matrix1989，9，1： | 1 |
| 5 | A comparative study of human periodontal ligament cell and gingival fibroblasts in vitro显示文摘 | Somerman M J Areher SY Imm GR | 1988 | J Dent Res1988，，1： | 1 |
| 6 | In vitro attachment of bacteria to extracts of cementum 显示文摘 | Somerman MJ Kagermeir AS Bowers MR | 1985 | J Oral Pathology1985，14，10： | 1 |
| 7 | Bone morphogenetic protein-2 inhibits differentiation and mineralization of cementoblasts in vitro显示文摘 | Zhao M Berry JE Somerman MJ | 2003 | J Dent Res2003，82，1： | 1 |
| 8 | Effects of minocycline on fibroblast Attachment and spreading显示文摘 | Somerman MJ Foster RA Vorsteg GM | | 0，，2： | 1 |
| 9 | Bone morphogenetic protein-2 inhibits differentiation and mineralization of ce- mentoblasts in vitro显示文摘 | Zhao M Berry JE Somerman MJ | 2003 | J Dent Res2003，82，1： | 1 |
| 10 | Bone morphogenetic proteins secreted by breast cancer cells upregulate bone sialoprotein expression in preosteoblast cells 显示文摘 | Bunyaratavej P Hullinger TG Somerman MJ | 2000 | Exp Cell Res2000，260，2： | 1 |
| 11 | Protein production by human gingival fibrnhlasts is enhanced by guanidine EDTA extracts of cementum 显示文摘 | Somerman MJ Archer SY Shteyer A | 1987 | J Periodont Res1987，22，1： | 1 |
| 12 | Bone morphogenetic pro tein-2 inhibits differentiation and mineralization of cemento blasts in vitro 显示文摘 | ZhaoM Berry JE Somerman MJ | 2003 | J Dent Res2003，82，1： | 1 |
| 13 | Role of osteopontin and bone sialopro- teln in cementogenesis 显示文摘 | MaeNeil RL Berry JE D_Errico JA Strayhorn C Piotrows- ki B Somerman MJ | 1995 | Connect Tissue Res1995，33，： | 1 |
| 14 | Bone morphogenetic proteins secreted by breast cancer cells upregulate bone sialoprotein expression in preosteoblast cells 显示文摘 | Bunyaratavej P Hullinger TG Somerman MJ | 2000 | Exp Cell Res2000，260，2： | 1 |
| 15 | Molecular and cell biology of cementum 显示文摘 | Saygin NE Giannobile WV Somerman MJ | 2000 | Periodontol 20002000，24，： | 1 |
| 16 | Effects of minocycline on fibroblast attachment and spreading显示文摘 | Somerman MJ Foster RA Vorsteg GM | 1988 | J Periodontol Res1988，23，： | 1 |
| 17 | Expression of extracelluar matrix proteins in human periodontal ligament cells during mineration in vitro 显示文摘 | Nohutcu RM Mc Cauley LK Somerman MJ | 1997 | J Periodontal1997，68，4： | 1 |
| 18 | Dentin sialoprotein (DSP) transcripts:Developmentally-sustained expression in odontoblasts and transient expression in pre-anleloblasts显示文摘 | Ritchie HH Berry JE Somerman MJ | 1997 | Eur J Oral Sci1997，105，51： | 1 |
| 19 | Effect of mi- nocycline on fibroblast attachment and spreading 显示文摘 | Somerman M J Foster RA Vorsteg GM | 1998 | J Periodontal Res1998，23，2： | 1 |
| 20 | Cell attachment activity of the 44 kilodahon bone phosphoprotein is not restricted to bone cells显示文摘 | Somerman M J Prince CW Butler WT | 1989 | Matrix1989，9，1： | 1 |
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