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| 1 | Inhibition of hepatitis C virus replication by single-stranded RNA structural mimics显示文摘AIM: To examine the effect of hepatitis C virus (HCV) structural mimics of regulatory regions of the genome on HCV replication.METHODS: HCV RNA structural mimics were constructed and tested in a HCV genotype 1b aBB7 replicon,and a Japanese fulminant hepatitis-1 (JFH-1) HCV genotype 2a infection model.All sequences were computer-predicted to adopt stem-loop structures identical to the corresponding elements in full-length viral RNA.Huh7.5 cells bearing the BB7 replicon or infected with JFH-1 virus were transfected with expression vectors generating HCV mimics and controls.Cellular HCV RNA and protein levels were quantified by real-time polymerase chain reaction and Western blotting,respectively.To evaluate possible antisense effects,complementary RNAs spanning a mimic were prepared.RESULTS: In the BB7 genotype 1b replicon system,mimics of the polymerase (NS-5B),X and BA regions inhibited replication by more than 90%,50%,and 60%,respectively.In the JFH-1 genotype 2 infection system,mimics that were only 74% and 46% identical in sequence relative to the corresponding region in JFH-1 inhibited HCV replication by 91.5% and 91.2%,respectively,as effectively as a mimic with complete identity to HCV genotype 2a.The inhibitory effects were confirmed by NS3 protein levels.Antisense RNA molecules spanning the 74% identical mimic had no significant effects.CONCLUSION: HCV RNA structural mimics can inhibit HCV RNA replication in replicon and infectious HCV systems and do so independent of close sequence identity with the target. | Robert Smolic Martina Smolic John H Andorfer Catherine H Wu Robert M Smith George Y Wu | 2010 | World Journal of Gastroenterology2010，16，17： | 2 |
| 2 | Targeting the Wnt Signaling Pathway in Liver Fibrosis for Drug Options:An Update显示文摘morbidity and mortality for healthcare systems worldwide.It imparts an enormous economic burden to societies,making continuous research and informational updates about its pathogenesis and treatment crucial.This review′s focus is on the current knowledge about the Wnt signaling path-way,serving as an important pathway in liver fibrosis development and activation of hepatic stellate cells(HSCs).Two types of Wnt pathways are distinguished,namely the ß-catenin-dependent canonical and non-canonical Ca^(2+) or planar cell polarity(PCP)-dependent pathway.The dynamic balance of physiologically healthy liver and hepatocytes is disturbed by repeated liver injuries.Activation of theß-catenin Wnt pathway prevents the regeneration of hepatocytes by the replacement of extracellular matrix(ECM),leading to the appearance of scar tissue and the formation of regenerated nodular hepatocytes,lacking the original function of healthy hepatocytes.Therefore,liver function is reduced due to the severely advanced disease.Selective inhibition ofß-catenin inhibits inflammatory processes(since chemokines and pro-inflammatory cytokines are produced during Wnt activation),reduces growth of activated HSCs and reduces collagen synthesis and angiogenesis,thereby reducing the progression of liver fibrosis in vivo.While the canonical Wnt pathway is usually inactive in a physiologically healthy liver,it shows activity during cell regeneration or renewal and in certain pathophysiological conditions,such as liver diseases and cancer.Targeted blocking of some of the basic components of the Wnt path-way is a therapeutic approach.These include the frizzled transmembrane receptor(Fz)receptors using the secreted frizzled-related protein family(sFRP),Fz-coreceptors low-density LRP 5/6 through dickkopf-related protein 1(DKK1)or niclosamide,glycogen kinase-3 beta(GSK-3β)using SB-216763,cyclic-AMP response element-binding protein(CBP)using PRI-724 and ICG-001,the lymphoid enhancer binding factor(LEF)/T cell-specific transcription factor(TCF)system as well as Wnt inhibitory factor 1(WIF1)and miR-17-5p using pinostilbene hydrate(PSH).Significant progress has been made in inhibiting Wnt and thus stopping the progression of liver fibrosis by diminishing key components for its action.Comprehending the role of the Wnt signaling pathway in liver fibrosis may lead to discovery of novel targets in liver fibrosis therapeutic strategies’development. | Kristina Duspara Kristina Bojanic Josipa Ivanusic Pejic Lucija Kuna Tea Omanovic Kolaric Vjera Nincevic Robert Smolic Aleksandar Vcev Marija Glasnovic Ines Bilic Curcic Martina Smolic | 2021 | Journal of Clinical and Translational Hepatology2021，9，6： | 2 |
| 3 | Long-Term Global Motion Estimation and Its Application for Sprite Coding,Content Description,and Segmentation显示文摘 | Aljoseha Smolic Thonms Sikora Jens-Rainer Ohm | 1999 | IEEE Transaction on Circuits and Systems for Technology1999，9，8： | 1 |
| 4 | Potential role of RNAi in the treatment of HCV infection显示文摘 | Volarevic M Smolic R Wu CH | 2007 | Expert Rev Anti Infect Ther2007，5，5： | 1 |
| 5 | The effects of multiview depth video eompressian on multiview rendering显示文摘 | MERKIE P MORVAN Y SMOLIC A | 2009 | Signal Processing: Image Communication2009，24，12： | 1 |
| 6 | Coding algorithms for 3DTV:a survey显示文摘 | SMOLIC A MUELLER K STEFANOSKI N | 2007 | IEEETransactions on Circuits and Systems for Video Tech-nology2007，17，11： | 1 |
| 7 | Coding algorith ms for 3DTV-A survey显示文摘 | Smolic A Muei LER K Stefanoski N et a1 | 2007 | IEEE Transactions on Circuits and Systems for Video Techneology2007，17，11： | 1 |
| 8 | Coding algo- rithms for 3DTV--A survey 显示文摘 | SMOLIC A MUELLER K STEFANOSKI N | 2007 | IEEE Transactions on Circuits and Systems for Video Technology2007，17，11： | 1 |
| 9 | Coding algo-rithms for 3DTV-a survey显示文摘 | Smolic A Mueller K Stefanoski N | 2007 | IEEE Transactions on Cir-cuitsand Systems for Video Technology2007，17，11： | 1 |
| 10 | Efficient prediction structures for multiview video coding 显示文摘 | Merkle P Smolic A Mueller K l | 2007 | IEEE Transactions on Circuits System for Video Technology2007，17，11： | 1 |
| 11 | Efficient prediction structures for multiview video coding 显示文摘 | Merkle P Smolic A Muller K | 2007 | IEEE Tran- sactions on Circuits and Systems for Video Technology2007，17，11： | 1 |
| 12 | Efficient predictionstructures for multiview video coding显示文摘 | Merkle P Smolic A Muller K | 2007 | IEEE Transac-tions on Circuits and Systems for VideoTechnology2007，17，11： | 1 |
| 13 | Efficient prediction structures for multiview video coding显示文摘 | Merkle P Smolic A Muller K | 2007 | IEEE Transactions on Circuits and Systems for Video Technology2007，17，11： | 1 |
| 14 | Potential role of RNAi in the treatment of HCV infection 显示文摘 | VOLAREVIC M SMOLIC R WU CH | 2007 | Expert Rev Anti Infect Ther2007，5，5： | 1 |
| 15 | Efficient prediction structures for multi-view video coding 显示文摘 | Merkle P Smolic A Muller K | 2007 | IEEE Transac- tions on Circuits and Systems for Video Technology2007，17，11： | 1 |
| 16 | Multi-view imaging and 3DTV 显示文摘 | KUBOTA A SMOLIC A MAGNOR M TANIMOTO M CHEN T ZHANC C | 2007 | IEEE Signal Processing Magazine2007，11，4： | 1 |
| 17 | Efficient prediction structures for multiview video coding显示文摘 | MERKLE P SMOLIC A MULLER K | 2007 | IEEE Transactions on Circuits and Systems for Video Technology2007，17，11： | 1 |
| 18 | Efficient prediction structures for multi-view video coding显示文摘 | Merkle P Smolic A Mueller K | 2007 | IEEE Trans on Circuits Syst Video Technol2007，17，11： | 1 |
| 19 | The effects of multiview depth video compression on multiview rendering 显示文摘 | Merkle P Morvan Y Smolic A | 2009 | Signal Processing: Image Communication2009，24，1： | 1 |
| 20 | Long-term global motion estimation and its application for sprite coding, content description, and segmentation 显示文摘 | Smolic A Sikora T Ohm J R | 1999 | IEEE Transactions on Circuits and Systems for Video Technology1999，9，8： | 1 |
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