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	题名	作者	年代	出处	被引量
1	Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic me- dium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reac- tion (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.	ZHENG Yi-xiong RINGE Jochen LIANG Zhong LOCH Alexander CHEN Li SITTINGER Michael	2006	Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，10：	8
2	Effect of growth factors on cell proliferation by human nasal septal chondrocytes cultured in monolayer显示文摘	Sittinger M Wilmes E	1994	Acta Otolaryngol( Stockh )1994，114，：	1
3	Artificial tissues in pedusion culture显示文摘	Sittinger M Schultz O Keyszer G	1997	Int J Artif Organs1997，20，1：	1
4	Tissue engineering and autologous transplant formation:practical approaches with resorbable biomaterials and new cell culture techniques显示文摘	Sittinger M Bujia J Rotter J	1996	Biomaterials1996，17，：	1
5	Joint cartilage regeneration by tissueengineering显示文摘	Sittinger M Perka C Schultz O	1999	Rheumato1999，58，3：	1
6	Thin film solar cell technology in Germany 显示文摘	Diehl W Sittinger V Szyszka B	2005	Surface & Coatings Technology2005，193，：	1
7	Impact of pharmaco- kinetic( CYP2C9 ) and pharmacodynamic ( VKORC1, F7, GGCX,CALU, EPHX1 )gene variants on the initiation and maintenance phases of phenprocoumon therapy 显示文摘	Luxembourg B Schneider K Sittinger K	2011	Thromb Haemost2011，105，1：	1
8	Encapsulation of artificial tissue in polyleetrolyte complexes:preliminary studies显示文摘	Sittinger M Lukanoff B Burmester GR	1996	Biomaterials1996，17，：	1
9	VKORC1 haplotypes and their impact on the inter- individual and inter- ethnical variability of oral anticoagulation显示文摘	Geisen C Watzka M Sittinger K	2005	Thromb Haemost2005，94，4：	1
10	Tissue engineering and autologous transplant formation:practical approaches with absorbable biomaterials and new culture techniques 显示文摘	Sittinger M Bujia J Rotter N	1996	Biomaterials1996，17，：	1
11	Engineering of cartilage tissue using bioresorbable polomer cowries in perfusion culture显示文摘	SITTINGER M BUJIA J MINUTH WW	1994	Biomaterials1994，15，6：	1
12	Secretion of gelatinases and activation of gelatinasc A(MMP-2)by human rheumatoid synodal fibroblasts显示文摘	Smolian H AurerA Sittinger M	2001	Biol Chem2001，382，：	1
13	显示文摘	Martino A Di Sittinger M Risbud M V	2005	Biomaterials2005，26，：	1
14	Scaffold based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems显示文摘	Hutmacher DW Sittinger M Risbud MV	2004	Trends Biotechnol2004，22，7：	1
15	Engineering of polymer-based grafts with cells derived from human nu- cleus pulposus tissue of the lumbar spine显示文摘	Hegewald AA Enz A Sittinger M	2011	Tissue Eng Regen Med2011，5，4：	1
16	Cryopreservation of artificial cartilage:viability and functional examination after thawing显示文摘	Lubke C Sittinger M Burmester GR	2001	Cells Tissues Organs2001，169，4：	1
17	Making bone:implant insertion into tissue-engineered bone for maxillary sinus floor augmentation-a preliminary report显示文摘	Schmelzeisen R Schimming R Sittinger M	2003	J Craniomaxillofac Surg2003，31，1：	1
18	Heat treatable indium tin oxide films deposited with high power pulse magnetron sputtering 显示文摘	Horstmann F Sittinger V Szyszka B	2009	Thin Solid Films2009，517，：	1
19	Tissue engineering:artificial tissue replacement containing vital components显示文摘	Sittinger M	1995	Laryngorhinootologie1995，74，11：	1
20	Thin film solar cell technology in Germany显示文摘	DIEHL W SITTINGER V SZYSZKA B	2005	Surface & Coatings Technology2005，，3：	1

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