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2篇 您的检索式：作者名="Siming GUAN"

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		题名	作者	年代	出处	被引量
	1	Effects of alendronate on the Notch1?RBP?Jκ signaling pathway in theosteogenic differentiation and mineralization of vascular smooth muscle cells显示文摘	Shaoqiong Zhou Xin Fang Huaping Xin Siming Guan	2013	Molecular Medicine Reports2013，，1：	1
	2	Construction of eukaryotic expression vector of humanarresten gene and its secreted expression in HEK 293 cells显示文摘The eukaryotic expression vector of human arresten gene was constructed and its secretive expression in human embryonic kidney(HEK 293)cells was detected.Human arresten gene was ampliﬁed from recombinant plasmid pGEM-Arr by polymerase chain reaction(PCR),and then digested with restriction endonucleases BamH I and EcoR I.The target fragment was inserted into the BamH I and EcoR I restriction sites of eukaryotic expression vector pSecTag2 to construct pST-AT.Restric-tion analysis and DNA sequencing indicated that the arresten gene was successfully inserted into pSecTag2.The recombinant plasmid was subsequently transfected into HEK 293 cells with LipofectAMINETM2000 Reagent,and the expression of the target gene was detected.Reverse transcription PCR(RT-PCR)revealed that the mRNA of the target gene was transcribed in the transfected HEK 293 cells.Western blot analysis veriﬁed that the recombinant protein in supernatants was correct.The supernatants of transfected cells were prepared,and 3-(4,5)-dimethylthiazol(-2-y1)-3,5-di-phenyltetrazoliumbromide(MTT)assay was carried out to assess their effects on the proliferation of human umbilical vein endothelial cells,which showed that the recombinant protein could signiﬁcantly suppress the proliferation of human umbilical vein endothelial cells in vitro.These results provided a solid foundation to explore the usage of arresten in tumor anti-angiogenic gene therapy.	Wei LI Zifang SONG Qichang ZHENG Jun XIONG Dan SHANG Siming GUAN Xiaogang SHU	2009	Frontiers of Medicine2009，3，3：	0

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