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| 1 | Development of a multiplex one-step real-time RT-PCR assay for the simultaneous detection of eight viruses associated with febrile rash illnesses显示文摘Fever and rash illnesses(FRIs)are a series of common diseaseswith fever and rashes as clinicalmanifestations,most of which are caused by viral infection.The rashes of FRIs are generally nonspecific;therefore it is difficult to identify FRIassociated viruses solely based on clinical symptoms.To achieve rapid and accurate identification of FRI pathogens,a multiplex one-step real-time reverse transcription-polymerase chain reaction(RT-PCR)assay was developed and evaluated in this study.Primers and probes were selected for the detection of measles virus(MeV),rubella virus(RV),human enterovirus(EV),varicella-zoster virus(VZV),dengue virus(DENV),human parvovirus B19(B19),Epstein-Barr virus(EBV),and human herpes virus 6(HHV-6),which cover the most common pathogenic viruses of FRIs.Detection of the eight FRI-associated viruses,which was divided into two groups/tubes,was simultaneously performed under universal optimized reaction conditions in multiplex one-step real-time RT-PCR assay.The multiplex realtime RT-PCR showed high sensitivity and specificity in detecting the eight FRI-associated viruses.The limits of detection(LODs)for the eight viruses were in the range of 47–177 copies/reaction,and no cross reactions for the eight FRIassociated viruses were found in the multiplex assay.In addition,the results of the multiplex real-time RT-PCR assay were consistent with the results of a monoplex real-time RT-PCR assay and sequencing for clinical specimens obtained from FRI patients.With its advantages of high efficiency and rapid and accurate diagnosis,multiplex real-time RT-PCR was very feasible for the early diagnosis of FRI pathogenic viruses and would be of great help for the proper treatment,monitoring,and initiation of preventive measures for FRI cases. | Aili Cui Shulei Wang Qiang Zhang Huiling Wang Zhen Zhu Aqian Li Qinqin Song Yanzhe Hao Jilan He Wenbo Xu Yan Zhang | 2020 | Biosafety and Health2020，2，2： | 5 |
| 2 | Several miRNAs derived from serum extracellular vesicles are potential biomarkers for early diagnosis and progression of Parkinson’s disease显示文摘Background:Blood-based test for predicting disease progression and early diagnosis of Parkinson’s disease(PD)is an unmet need in the clinic.The profiles of microRNAs(miRNAs)are regarded as potential diagnostic biomarkers for human diseases,whereas miRNAs in the periphery are susceptible to the influence of various components.MiRNAs enriched in serum extracellular vesicles(EVs)have demonstrated disease-specific advantages in diagnosis due to their high abundance,stability and resistance to degradation.This study was aimed to screen differentially expressed EV-derived miRNAs between healthy controls and PD patients to aid in diagnosis of PD.Methods:A total of 31 healthy controls and 72 patients with a diagnosis of PD at different Hoehn and Yahr stages in Tangdu Hospital were included.In total,185 differentially expressed miRNAs were obtained through RNA sequencing of serum EVs as well as edgeR and t-test analyses.Subsequently,the weighted gene co-expression network analysis(WGCNA)was utilized to identify the commonly expressed miRNAs in all stages of PD by constructing connections between modules,and specifically expressed miRNAs in each stage of PD by functional enrichment analysis.After aligning these miRNAs with PD-related miRNAs in Human miRNA Disease Database,the screened miRNAs were further validated by receiver operating characteristic(ROC)curves and quantitative real-time polymerase chain reaction(qRT-PCR)using peripheral blood EVs from 40 more participants.Results:WGCNA showed that 4 miRNAs were commonly associated with all stages of PD and 13 miRNAs were specifically associated with different stages of PD.Of the 17 obtained miRNAs,7 were validated by ROC curve analysis and 7 were verified in 40 more participants by qRT-PCR.Six miRNAs were verified by both methods,which included 2 miRNAs that were commonly expressed in all stages of PD and 4 miRNAs that were specifically expressed in different stages of PD.Conclusions:The 6 serum EV-derived miRNAs,hsa-miR-374a-5p,hsa-miR-374b-5p,hsa-miR-199a-3p,hsa-miR-28-5p,hsa-miR-22-5p and hsa-miR-151a-5p,may potentially be used as biomarkers for PD progression and for early diagnosis of PD in populations. | Shulei He Lu Huang Ci Shao Tiejian Nie Li Xia Bozhou Cui Fangfang Lu Lin Zhu Bolin Chen Qian Yang | 2021 | Translational Neurodegeneration2021，10，3： | 1 |
| 3 | A Mesh Deformation Method with Multiple Edit-ing Granularities显示文摘 | Zhengjie Deng Fengwei Wang Guoyuan Chen Chun Shi Shuqian He Shulei Wu | 2013 | Applied Mechanics and Materials2013，，3： | 1 |
| 4 | Rockburst mechanism and the law of energy accumulation and release in mining roadway: a case study显示文摘The rockburst dynamic disasters in the process of deep coal mining become more and more serious.Taking the rockburst occurred in the 23130 working face of Yuejin Coal Mine as the engineering background,we study the characteristics of mining stress feld around roadway,the plastic failure morphological characteristics of surrounding rock and the accumulation/release law of elastic energy before and after burst.An analysis model quantitatively describing the physical process of rockburst in the mining roadway is established,and the calculation method of dynamic release of elastic energy in the physical process of rockburst is educed.The mechanism of rockburst in mining roadway is revealed.The results show that an“L-shaped”stress concentration zone is formed within 100 m of the 23130 working face,and the principal stress ratio of the surrounding rock of the transportation roadway is 2.59–4.26.The change of the direction of the maximum principal stress has a signifcant efect on the burst appearance characteristics.The failure strength of diferent sections of the mining roadway is characterized by the elastic energy release value.With the increase of the working face distance,the elastic energy released by burst failure and the expansion variation of failure boundary radius show a nonlinear variation law that tends to decrease steadily after sharp fuctuation.The closer to the working face,the higher the burst risk.At a distance of 10 m from the working surface,the maximum principal stress reaches its maximum value.The butterfy-shaped failure system generated by the surrounding rock of the roadway has energy self-sustainability,and the elastic energy released by the sudden expansion of the butterfy leaf is enough to cause a burst damage of 1.9 magnitude.This work could provide theoretical support for the prediction and prevention of rockburst. | Feng Du Ji Ma Xiaofei Guo Tianfeng Wang Xiaohang Dong Jiashuo Li Shulei He Dilinaer Nuerjuma | 2022 | International Journal of Coal Science & Technology2022，9，5： | 1 |
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