维普期刊资源整合服务平台（V6.5）

维普中文期刊产品整合服务

1篇 您的检索式：作者名="Shinitiro Oda"

选中清除导出查看参考文献查看引证文献引用追踪共1页首页上一页第1页下一页末页 /1 跳转

		题名	作者	年代	出处	被引量
	1	Cloning and characterization of gene-resistant analogs(RGAs)involved in rust(Puccinia psidii) resistance in Eucalyptus grandis显示文摘Disease-resistant genes play an important role in defending against a variety of pathogens and insect pests in plants. Most of the disease-resistant genes encode proteins with conserved leucine rich repeat and nucleotide binding site domains. In this study, we cloned and characterized gene-resistant analogs(RGAs) from Eucalyptus grandis using degenerate PCR, with primers specifically targeting these two domains. The amplified fragments were cloned into the p GEM-T vector and transformed into Escherichia coli. Among the 90 clones obtained, 13 were sequenced and compared with each other and with previously identified gene-resistant diseases. A BLASTX search in Gen Bank revealed high similarities among the conserved domains of these cloned genes with RGA genes.Some clones, however, showed no significant similarity with DNA sequences in Gen Bank. Southern blotting analysis identified several polymorphic RFLP loci between distinct genotypes. However, none of them co-segregated with the Puccinia psidii Winter resistance gene 1(Ppr1) in a population study.	Marcelo Luiz Laia Acelino Couto Alfenas Sergio Hermínio Brommonschenkel Shinitiro Oda Eduardo José de Melo Inaê Mariêde Araú jo Silva Janaína Fernandes Gonalves Ariadne Marques	2015	Journal of Forestry Research2015，26，3：	0

返回顶部每页显示：

共1页首页上一页第1页下一页末页 /1 跳转

goolge学术搜索-维普

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费