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您的检索式：作者名="Shinian You"
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| 1 | Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis显示文摘We have previously demonstrated that exosomes derived from cancer-associated fibroblasts(CAFs)promote bladder cancer(BC)cell proliferation and invasion by transferring LINC00355.In this study,the molecular mechanisms underlying the pro-bladder cancer action of exosomal LINC00355 were explored.CAFs were obtained from BC tumor tissues,and normal fibroblasts(NFs)were obtained from adjacent normal tissues.Human BC cell lines(T24 and 5367)were incubated with NF-Exo(exosomes from NFs),CAF-Exo(exosomes from CAFs),CAFsi-Ctrl-Exo(exosomes from si-Ctrl-transfected CAFs),and CAFsi-LINC00355-Exo(exosomes from si-LINC00355-transfected CAFs).BC cell proliferation and invasion were evaluated by MTT and Transwell assays,respectively.The interaction between miR-15a-5p and LINC00355 or HMGA2 was examined by online bioinformatics analysis and luciferase activity assay.Results showed that HMGA2 is a direct target of miR-15a-5p,and LINC00355 functions as a sponge of miR-15a-5p to upregulate HMGA2 expression.The promoting effects of CAF-Exo on HMGA2 expression,cell proliferation,and cell invasion were hindered when LINC00355 expression was inhibited in BC cells.These promoting effects were also hindered when miR-15a-5p was overexpressed or HMGA2 was silenced in BC cells.In conclusion,exosomal LINC00355 derived from CAFs promotes BC cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis. | Yangyang Zhang Guangyue Luo Shinian You Ligang Zhang Chaozhao Liang Xianguo Chen | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 4 |
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