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| 1 | Transgenic expression of MYB15 confers enhanced sensitivity to abscisic acid and improved drought tolerance in Arabidopsis thaliana显示文摘Abiotic stresses cause serious crop losses. Knowledge on genes functioning in plant responses to adverse growth conditions is essential for developing stress tolerant crops. Here we report that transgenic expression of MYB15, encoding a R2R3 MYB transcription factor in Arabidopsis thaliana, conferred hypersensitivity to exogenous abscisic acid (ABA) and improved tolerance to drought and salt stresses. The promoter of MYB15 was active in not only vegetative and reproductive organs but also the guard cells of stomata. Its transcript level was substantially upregulated by ABA, drought or salt treatments. Compared with wild type (WT) control, MYB15 overexpression lines were hypersensitive to ABA in germination assays, more susceptible to ABA-elicited inhibition of root elongation, and more sensitive to ABA-induced stomatal closure. In line with the above findings, the transcript levels of ABA biosynthesis (ABA1, ABA2), signaling (ABI3), and responsive genes (AtADH1, RD22, RD29B, AtEM6) were generally higher in MYB15 overexpression seedlings than in WT controls after treatment with ABA. MYB15 overexpression lines displayed improved survival and reduced water loss rates than WT control under water deficiency conditions. These overexpression lines also displayed higher tolerance to NaCl stress. Collectively, our data suggest that overexpression of MYB15 improves drought and salt tolerance in Arabidopsis possibly by enhancing the expression levels of the genes involved in ABA biosynthesis and signaling, and those encoding the stress-protective proteins. | Zhenhua Ding Shiming Li Xueli An Xin Liu Huanju Qin Daowen Wang | 2009 | Journal of Genetics and Genomics2009，36，1： | 42 |
| 2 | Significance of dredging on sediment denitrification in Meiliang Bay,China:A year long simulation study显示文摘An experiment for studying the effects of sediment dredging on denitrification in sediments was carried out through a one-year incubation of undredged (control) and dredged cores in laboratory. Dredging the upper 30 cm of sediment can significantly affect physico-chemical characteristics of sediments. Less degradation of organic matter in the dredged sediments was found during the experiment. Denitrification rates in the sediments were estimated by the acetylene blockage technique, and ranged from 21.6 to 102.7 nmol N2/(g dry weight (dw)·hr) for the undredged sediment and from 6.9 to 26.9 nmol N2/(g dw·hr) for dredged sediments. The denitrification rates in the undredged sediments were markedly higher (p<0.05) than those in the dredged sediments throughout the incubation, with the exception of February 2006. The importance of various environmental factors on denitrification was assessed, which indicated that denitrification was regulated by temperature. Nitrate was probably the key factor limiting denitrification in both undredged and dredged sediments. Organic carbon played some role in determining the denitrification rates in the dredged sediments, but not in the undredged sediments. Sediment dredging influenced the mineralization of organic matter and denitrification in the sediment; and therefore changed the pattern of inherent cycling of nitrogen. | Jicheng Zhong Chengxin Fan Lu Zhang Edward Hall Shiming Ding Bao Li Guofeng Liu | 2010 | Journal of Environmental Sciences2010，22，1： | 40 |
| 3 | Speciation of organic phosphorus in a sediment profile of Lake Taihu I:Chemical forms and their transformation显示文摘Organic phosphorus(nonreactive P,NRP)is a major component of P in sediments,but information about its chemical forms and dynamic transformation is limited.The chemical forms and dynamic behaviors of NRP in a sediment profile from Lake Taihu,a freshwater and eutrophic lake in China,were investigated.Five forms of NRP in the sediments were extracted based on a chemical fractionation technique,including easily labile NRP(NaHCO3-NRP),reactive metal oxide-bound NRP(HCl-NRP),humic acid-associated NRP(NaOH-NRPHA),fulvic acid-associated NRP(NaOH-NRPFA)and residual NRP(Res-TP).There were notable transformations with increasing sediment depth from the labile NaHCO3-NRP and NaOH-NRP pools to the recalcitrant HCl-NRP and Res-TP pools,which caused the NRP to become increasingly recalcitrant as the early diagenetic processes proceeded.Further analyses showed that the relative changes in contents of organic matter and reactive Fe oxides in the sediment profile triggered a competition for binding NRP fractions and led to the transformation of NRP.The results highlighted the importance of abiotic processes in regulating the diagenesis of organic P and its stability in sediments. | Di Xu Shiming Ding Bin Li Xiuling Bai Chengxin Fan Chaosheng Zhang | 2013 | Journal of Environmental Sciences2013，25，4： | 7 |
| 4 | Investigation of potential interferences on the measurement of dissolved reactive phosphate using zirconium oxide-based DGT technique显示文摘A diffusive gradients in thin films (DGT) technique based on hydrous zirconium oxide (Zr-oxide) has been recently developed for the measurement of dissolved reactive phosphate (DRP). In this study, the detailed performance of the DGT technique is reported. Spiking experiments revealed that several orthophosphate monoester compounds contributed to the Zr-oxide DGT measurements of DRP. However, such a phenomenon is unlikely to occur during field conditions due to the low concentration of organic P in typical natural waters. The presence of Cl- (up to 106 g/L), SO42- (up to 16 g/L), HCO3- (up to 817 g/L), and AsO2- and AsO 3 4 (both up to 1 mg As/L) in solutions had negligible effects on the measurement of DRP. The threshold concentrations of Cl-, SO42- and HCO3- have been increased from previous reports for the measurements of DRP using other adsorbent-based DGT techniques. The capacity for DGT measurements of DRP decreased with increasing solution pH (4.2-9.2). The lowest capacity (95 μg P/m2 at pH 9.2) was still greater than that of other DGT techniques that are usually used for the measurement of DRP (2-12 μg P/cm2 ). The Zr-oxide binding gel could be stored for up to 2 years without any aging effect. This period of validity was considerably longer than the ferrihydrite binding gel that is commonly used in present DGT devices (6 months). The field application revealed that the concentrations of DRP measured in three fresh water samples using the Zr-oxide DGT technique were in agreement with those of the traditional colorimetric method. | Qin Sun Yifei Chen Di Xu Yan Wang Shiming Ding | 2013 | Journal of Environmental Sciences2013，25，8： | 3 |
| 5 | Speciation of organic phosphorus in a sediment profile of Lake Taihu Ⅱ.Molecular species and their depth attenuation显示文摘The understanding of organic phosphorus(P) dynamics in sediments requires information on their species at the molecular level,but such information in sediment profiles is scarce.A sediment profile was selected from a large eutrophic lake,Lake Taihu(China),and organic P species in the sediments were detected using solution phosphorus-31 nuclear magnetic resonance spectroscopy(31 P NMR) following extraction of the sediments with a mixture of 0.25 mol/L NaOH and 50 mmol/L EDTA(NaOH-EDTA) solution.The results showed that P in the NaOH-EDTA extracts was mainly composed of orthophosphate,orthophosphate monoesters,phospholipids,DNA,and pyrophosphate.Concentrations of the major organic P compound groups and pyrophosphate showed a decreasing trend with the increase of depth.Their half-life times varied from 3 to 27 years,following the order of orthophosphate monoesters > phospholipids DNA > pyrophosphate.Principal component analysis revealed that the detected organic P species had binding phases similar to those of humic acid-associated organic P(NaOH-NRP HA),a labile organic P pool that tends to transform to recalcitrant organic P pools as the early diagenetic processes proceed.This demonstrated that the depth attenuation of the organic P species could be partly attributed to their increasing immobilization by the sediment solids,while their degradation rates should be significantly lower than what were suggested in previous studies. | Shiming Ding Di Xu Xiuling Bai Shuchun Yao Chengxin Fan Chaosheng Zhang | 2013 | Journal of Environmental Sciences2013，25，5： | 3 |
| 6 | Equilibrium Phase Constituents and Discontinuous Precipitation Behavior of Al-Zn-Cu Alloy with Symmetrical Composition显示文摘Through microstructure observation and X-ray diffraction analysis, the equilibrium phase constituents of Al-Zn alloythat contains 2 at. pct Cu at room temperature have been determined as Al-based solid solution (α), Zn-based solidsolution and Al4Cu3Zn phase (T′-phase), which are different from α phase, Zn phase and CuZn4 phase originallybelieved. It is determined that the products of discontinuous precipitation transformation below 277℃ are not theequilibrium phase constituents, but the metastable phases made up of α phase, Zn phase and CuZn4 phase. Thephase constituents after discontinuous precipitation of AlZn-2Cu alloy would transform to the ones in equilibriumstatus: Al-based solid solution (c) in fcc structure, Zn-based solid solution in hcp structure and Al4Cu3Zn phase(T′-phase) ultimately through plastic deformation at room temperature and re-heating treatment below 277℃. | Shiming HAO, Yuping REN, Hua DING, Dapeng WANG, Gang ZHAO and Hongxiao LISchool of Materials and Metallurgy, Northeastern University, Shenyang 110004, China | 2003 | Journal of Materials Science & Technology2003，19，5： | 3 |
| 7 | Insights Into the Interfacial Degradation of High-Voltage All-Solid-State Lithium Batteries显示文摘Poly(ethylene oxide)(PEO)-based solid polymer electrolyte(SPE)is considered as a promising solid-state electrolyte for all-solid-state lithium batteries(ASSLBs).Nevertheless,the poor interfacial stability with high-voltage cathode materials(e.g.,LiCoO_(2))restricts its application in high energy density solid-state batteries.Herein,high-voltage stable Li_(3)AlF_(6) protective layer is coated on the surface of LiCoO_(2) particle to improve the performance and investigate the failure mechanism of PEO-based ASSLBs.The phase transition unveils that chemical redox reaction occurs between the highly reactive LiCoO_(2) surface and PEO-based SPE,resulting in structure collapse of LiCoO_(2),hence the poor cycle performance of PEO-based ASSLBs with LiCoO_(2) at charging voltage of 4.2 V vs Li/Li+.By sharp contrast,no obvious structure change can be found at the surface of Li_(3)AlF_(6)-coated LiCoO_(2),and the original layered phase was well retained.When the charging voltage reaches up to 4.5 V vs Li/Li+,the intensive electrochemical decomposition of PEO-based SPE occurs,leading to the constant increase of cell impedance and directly causing the poor performance.This work not only provides important supplement to the failure mechanism of PEO-based batter-ies with LiCoO_(2),but also presents a universal strategy to retain structure stability of cathode-electrolyte interface in high-voltage ASSLBs. | Jiawen Li Yuchen Ji Haoran Song Shiming Chen Shouxiang Ding Bingkai Zhang Luyi Yang Yongli Song Feng Pan | 2022 | Nano-Micro Letters2022，14，12： | 2 |
| 8 | Environmental biogeochemical behaviors of rare earth elements in soil–plant systems显示文摘 | Tao Liang Shen Zhang Lijun Wang Hsiang-Te Kung Yuqi Wang Aitang Hu Shiming Ding | 2005 | Environmental Geochemistry and Health2005，，4： | 2 |
| 9 | Evaluation of SARS-CoV-2-Neutralizing Nanobody Using Virus Receptor Binding Domain-Administered Model Mice显示文摘Due to the rapid spread of coronavirus disease 2019(COVID-19),there is an urgent requirement for the development of additional diagnostic tools for further analysis of the disease.The isolated nanobody Nb11-59 binds to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)reeptor-binding domain[RBD]with high afinity to neutralize the virus and block the angiotensin-converting enzyme 2-(ACE2-)RBD interaction.Here,we introduce a novel nanobody-based radiotracer named^(68)Ga-Nb1159.The radiotracer retained high affinity for the RBD and showed reliable radiochemical characteristics both in vitro and in vivo.Predinical positron emission tomography(PET)studies of Ga-Nb1159 in mice revealed is rapid dearance from circulation and robust uptake into the renal and urinary systems.Fortunately,^(68)Ga-Nb1159 could speifcally reveal the distribution of the RBD in mice.This sudy also helped to evaluate the pharmacodynamic efects of the neutralizing nanobody.Moreover,^(68)Ga-Nb1159 may be a promising l0ol lo explore the distribution of the RBD and improve the understanding of the virus.In particular,this study identified a novel molecular radioagent and established a reliable evaluation method for speifially investigating the RBD through noninvasive and visual PET technology. | Song Liu Guanghui Li Lei Ding Jin Ding Qian Zhang Dan Li Xingguo Hou Xiangxing Kong Jing Zou Shiming Zhang Hongbin Han Yakun Wan Zhi Yang Hua Zhu | 2022 | Research2022，，2： | 2 |
| 10 | Evaluation of in situ capping with clean soils to control phosphate release from sediments显示文摘 | XU Di DING Shiming SUN Qin | 2012 | Sci Total Environ2012，438，： | 1 |
| 11 | PHASE EQUILIBRIA OF α_2(α)/γ IN Ti-Al-Nb TERNARY SYSTEM显示文摘PHASEEQUILIBRIAOFα2(α)/γINTiAlNbTERNARYSYSTEM①DingJinjun,ZhaoGang,HaoShimingDepartmentofMaterialsScienceandEngineringSchool... | Ding Jinjun, Zhao Gang, Hao Shiming Department of Materials Science and Engineering School of Materials and Metallurgy, Northeastern University, Shenyang 110006 | 1997 | 中国有色金属学会会刊:英文版1997，7，4： | 1 |
| 12 | Caution Needed in Pretreatment of Sediments for Refining Phosphorus-31 Nuclear Magnetic Resonance Analysis: Results from a Comprehensive Assessment of Pretreatment with Ethylenediaminetetraacetic Acid显示文摘 | Ding ShiMing Bai Xiuling Fan Chengxin Zhang Lu | 2010 | EN2010，，5： | 1 |
| 13 | Evaluation of in situ capping with clean soils to control phosphate release from sediments显示文摘 | Di Xu Shiming Ding Qin Sun Jicheng Zhong Wei Wu Fei Jia | 2012 | Science of the Total Environment2012，，： | 1 |
| 14 | Assessment on contaminations in sediments of an intake and the inflow canals in Taihu Lake, China显示文摘作为在中国的第二个最大的淡水湖,泰胡·莱克在湖附近提供水供应给约 3200 万个居民。然而,戏剧性地增加了污染威胁了在最近的年里喝水供应的安全。在现在的学习,我们在 Gonghu 海湾在吸入和流入运河的沉积调查了营养素和重金属的污染,泰胡·莱克。而且,我们也在污染的空间分发特征上检验了人的活动的影响。我们的结果证明吸入与流入运河相比介绍了磷和氮的相对更低的集中。然而,在吸入的沉积的 Cr, Ni, Cu, Zn 和 Pb 的集中超过了最低效果水平(LEL ) 价值,显示潜在的风险到喝水资源。另外,在 Tianji 运河和 Jinshu 运河的沉积的 Ni 的集中超过了严重效果水平(SEL ) 价值。更重要地,在 Tianji 运河的沉积的 Cu 的集中超过了价值三次 SEL。Multivariate 统计分析证实国内污水首先贡献了滋养的累积,并且电子垃圾的漏在沉积统治了金属的丰富。一起拿,更多的努力应该被作在泰胡·莱克保证水资源的安全,特别为国内污水和工业废水的处理。 | Xiaolong WANG Shiming DING Qi ZHANG Weiping HU | 2015 | Frontiers of Environmental Science & Engineering2015，9，4： | 1 |
| 15 | Accumulation and fractionation of rare earth elements(REEs)in wheat:controlled by phosphate precipitationcell wall absorption and solution complexation显示文摘 | ShiMing Ding Tao Liang ChaoSheng Zhange | 2005 | Journal of Experimental Botany2005，56，420： | 1 |
| 16 | Accumulation and fractionation of rare earth elements (REEs)in wheat: controlled by phosphate precipitation cell wall absorption and solution complexation显示文摘 | ShiMing Ding Tao Liang ChaoSheng Zhang | 2005 | Journal of Experimental Botany2005，56，420： | 1 |
| 17 | Measurement of dis- solved reactive phosphorus using the diffusive gradients in thin films technique with a high-capacity binding phase显示文摘 | DING Shiming XU Di SUN Qin | 2010 | Environmental Science & Technology2010，44，21： | 1 |
| 18 | A high resolution dial- ysis technique for rapid determination of dissolved reactive phosphate and ferrous iron in pore water of sediments显示文摘 | XU Di WU Wei DING Shiming | 2012 | Sci- ence of the Total Environment2012，，： | 1 |
| 19 | Gel-based colora- tion technique for the submillimeter-scale imaging of labile phosphorus in sediments and soils with diffusive gradients in thin films显示文摘 | DING Shiming WANG Yan XU Di | 2013 | Environmental Science & Technology2013，47，14： | 1 |
| 20 | High resolution simul taneous measurements of dissolved reactive phosphorus anddissolved sulfide: the first observation of their simultaneous release in sedimcnts显示文摘 | DING Shiming SUN Qin XU Di | 2012 | Environmental Science & Technolo- gy2012，46，15： | 1 |
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