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| 1 | The interaction among gut microbes,the intestinal barrier and short chain fatty acids显示文摘The mammalian gut is inhabited by a massive and complicated microbial community, in which the hostachieves a stable symbiotic environment through the interdependence, coordination, reciprocal constraintsand participation in an immune response. The interaction between the host gut and themicrobiota is essential for maintaining and achieving the homeostasis of the organism. Consequently, guthomeostasis is pivotal in safeguarding the growth and development and potential productive performanceof the host. As metabolites of microorganisms, short chain fatty acids are not only the preferredenergy metabolic feedstock for host intestinal epithelial cells, but also exert vital effects on antioxidantsand the regulation of intestinal community homeostasis. Herein, we summarize the effects of intestinalmicroorganisms on the host gut and the mechanisms of action of short chain fatty acids on the fourintestinal barriers of the organism, which will shed light on the manipulation of the intestinal communityto achieve precise nutrition for specific individuals and provide a novel perspective for theprevention and treatment of diseases. | Jiayu Ma Xiangshu Piao Shad Mahfuz Shenfei Long Jian Wang | 2022 | Animal Nutrition2022，，2： | 15 |
| 2 | Determination of the available energy,standardized ileal digestibility of amino acids of fermented corn germ meal replacing soybean meal in growing pig diets显示文摘Three experiments were conducted to compare the digestible(DE),metabolizable energy(ME),and standardized ileal digestibility(SID)of amino acids(AA)in fermented corn germ meal(FCGM)and soybean meal(SBM),and evaluate the effects of FCGM replacing SBM in growing pig diets.In Exp.1,18 barrows with initial body weight(BW)of 60.2±3.40 kg were randomly allotted to 3 treatments with 6 replicates per treatment.The control diet used corn as the only energy ingredient,and 2 test diets were made by replacing 25.8%of corn with FCGM or SBM.The DE and ME of FCGM were less(P<0.01)than those of SBM.In Exp.2,18 barrows(59.3±2.52 kg BW)with ileal T-cannulas were randomly allotted to 3 treatments with 6 replicates per treatment.The SID of arginine,tryptophan and proline were greater(P<0.05)and the tyrosine was less(P=0.01)in SBM compared with FCGM.In Exp.3,144 growing pigs(56.1±5.22 kg BW)were randomly allotted to 4 treatments with 6 pens per treatment(3 barrows and 3 gilts per pen).Four diets(FCGM0,FCGM10,FCGM20 and FCGM30)were formulated using FCGM to replace 0%,10%,20%and 30%of SBM,respectively.The ME and SID values of AA of SBM and FCGM were determined by Exp.1 and 2.Results showed that increasing FCGM inclusion quadratically(P<0.05)increased the average daily gain(ADG),average daily feed intake,and the levels of serum immunoglobulin G(IgG)and urea nitrogen,and linearly(P<0.05)increased the serum IgM,the propanoic acid,butyric acid,total volatile fatty acid(VFA)and the Shannon index of microbiota in feces.Besides,the relative abundance of genus Streptococcus in FCGM0,Lactobacillus in FCGM10 and Lachnospiraceae in FCGM30 were increased(P<0.05)compared with other treatments.In conclusion,we recommend replacing 11.80%of SBM with FCGM to obtain the optimal ADG of growing pigs.Moreover,as the ratio of FCGM replacing SBM increased in diet,the immunity,intestinal microbiota and total VFA composition of growing pigs were improved. | Tengfei He Yuhui Zheng Xiangshu Piao Shenfei Long | 2022 | Animal Nutrition2022，，2： | 6 |
| 3 | Microencapsulated essential oils combined with organic acids improves immune antioxidant capacity and intestinal barrier function as well as modulates the hindgut microbial community in piglets显示文摘Background:The objective of this experiment was to evaluate the effect of a combination of microencapsulated essential oils and organic acids(MOA)on growth performance,immuno-antioxidant status,intestinal barrier function and microbial structure of the hindgut in piglets.A total of 120 piglets(Duroc×[Landrace×Yorkshire];weighted 7.66±1.79 kg,weaned at d 28)were randomly selected and allocated to 3 treatments with 4 replicates per group and 10 piglets per replicate according to the initial body weight and gender.The dietary treatments were as follows:1)basal diet(Ctrl);2)Ctrl+chlortetracycline(75 mg/kg)(AGP);3)Ctrl+MOA(1500 mg/kg).The experiment period was lasted for 21 d.Results:Compared to the Ctrl group,dietary supplemented MOA alleviated(P<0.05)the diarrhea rate from d 12 to 21,enhanced(P<0.05)the concentration of serum interlukin-10 and glutathione peroxidase in piglets on d 11 after weaning and serum superoxide dismutase in 21-day piglets.The MOA group also improved(P<0.05)the apparent digestibility of dry matter(DM),organic matter(OM)and gross energy(GE),up-regulated(P<0.05)the mRNA expression level of occludin,claudin-1 and mucin-2 in ileum and increased(P<0.05)the contents of propionic and butyric acids in the cecum of piglets.The MOA group modulated the cecal and colonic microbial community structure and increased(P<0.05)the abundance of Faecalibacterium and Muribaculaceae in cecum and Streptococcus and Weissella in colon.Additionally,AGP group decreased(P<0.05)apparent digestibility of DM,OM and GE as well as down-regulated(P<0.05)relative gene expression level of claudin-1 in duodenum and jejunum,ZO-1 and mucin-1 in jejunum of piglets.Conclusion:In summary,dietary supplemented MOA alleviated diarrhea and improved nutrient apparent digestibility in piglets via enhancing immuno-antioxidant properties,increasing digestive enzyme activity,upregulating the expression of intestinal barrier-related genes,and modifying the microbial community structure of the cecum and colon.Therefore,dietary supplementation with MOA as an alternative to antibiotics was feasible to improve intestinal health of piglets in practical production. | Jiayu Ma Shenfei Long Jian Wang Jie Gao Xiangshu Piao | 2022 | Journal of Animal Science and Biotechnology2022，13，4： | 5 |
| 4 | Strain-rate effect on initial crush stress of irregular honeycomb under dynamic loading and its deformation mechanism显示文摘The seemingly contradictory understandings of the initial crush stress of cellular materials under dynamic loadings exist in the literature, and a comprehensive analysis of this issue is carried out with using direct information of local stress and strain. Local stress/strain calculation methods are applied to determine the initial crush stresses and the strain rates at initial crush from a cell-based finite element model of irregular honeycomb under dynamic loadings. The initial crush stress under constant-velocity compression is identical to the quasi-static one, but less than the one under direct impact, i.e. the initial crush stresses under different dynamic loadings could be very different even though there is no strain-rate effect of matrix material. A power-law relation between the initial crush stress and the strain rate is explored to describe the strain-rate effect on the initial crush stress of irregular honeycomb when the local strain rate exceeds a critical value, below which there is no strain-rate effect of irregular honeycomb. Deformation mechanisms of the initial crush behavior under dynamic loadings are also explored.The deformation modes of the initial crush region in the front of plastic compaction wave are different under different dynamic loadings. | Peng Wang Zhijun Zheng Shenfei Liao Jilin Yu | 2018 | Acta Mechanica Sinica2018，34，1： | 3 |
| 5 | In situ probing of cell-cell communications with surface- enhanced Raman scattering (SERS) nanoprobes and microfluidic networks for screening of immunotherapeutic drugs显示文摘为癌症免疫疗法发现新奇的药要求一在屏蔽允许的站台的 vitro 药柔韧房间房间通讯的直接探查。这里,我们联合了散布的提高表面的拉曼(重量的单位) 有 microfluidic 的 nanoprobes 联网在 situ 监视癌症免疫者系统细胞间的通讯。microfluidic 站台与癌症房间在有免疫力的房间上面连接,在癌症房间分泌物充当表明调停人的地方。首先, gold@silver 核心壳 nanorods 被采用为发信号的分子的分析制作重量的单位 immunoprobes。在肿瘤微型环境的多重癌症分泌物是分析由的份量上一帮助重量的单位三维(3D ) 有高敏感(1 ng/mL ) 的 barcode 免疫分析。在在薄片上房间增长试金,第二,多重抑制免疫力的蛋白质由房间被发现禁止有免疫力的房间的激活的癌症藏匿了,显示站台模仿癌症免疫者系统通讯的生理的过程。而且,潜在的药候选人在这个平台上被测试。量的重量的单位免疫分析被执行在调整癌症房间和有免疫力的房间的活动的分泌物行为评估药功效。这试金显示出这个平台的适用性为在 vitro 药屏蔽。充分综合、高度自动化的 SERS-microfluidic 站台将为探查细胞间的通讯成为一个强大的分析工具并且应该加速发现和新奇的药的临床的确认,这被期望。 | Lei Wu Zhuyuan Wang Yizhi Zhang Jiayuan Fei Hui Chen Shenfei Zong Yiping Cui | 2017 | Nano Research2017，10，2： | 3 |
| 6 | Distinguishing breast cancer cells using surface-enhanced Raman scattering显示文摘 | Jing Yang Zhuyuan Wang Shenfei Zong Chunyuan Song Ruohu Zhang Yiping Cui | 2012 | Analytical and Bioanalytical Chemistry2012，，3： | 1 |
| 7 | Dynamic crushing of 2D cellular structures:local strain field and shock wave velocity显示文摘 | LIAO Shenfei ZHENG Zhijun YU Jilin | 2013 | International Journal of Impact Engineering2013，57，1： | 1 |
| 8 | A SERS-based immunoassay with highly increased sensitivity using gold/silver core-shell nanorods显示文摘 | Lei Wu Zhuyuan Wang Shenfei Zong Zhi Huang Puyang Zhang Yiping Cui | 2012 | Biosensors and Bioelectronics2012，，1： | 1 |
| 9 | Silica coated gold nanoaggregates prepared by reverse microemulsion method: Dual mode probes for multiplex immunoassay using SERS and fluorescence显示文摘 | Zhuyuan Wang Shenfei Zong Hui Chen Hui Wu Yiping Cui | 2011 | Talanta2011，，： | 1 |
| 10 | 稚嫩的天空显示文摘偷空一个人躲在阳台,享受着冬日里碧空下浓浓的暖阳,不停的打着哈气,看着窗外那片有限的空间,记忆却无限的延伸着,驾着那七彩的祥云飘过城市的天空,绕过一座座高楼大厦来到曾经游戏的地方,飘回儿时的乐园。 | shenfei | 2009 | 健康大视野2009，，14： | 0 |
| 11 | Universal peroxidase-like strategy for sensitive glucose detection in complex matrix显示文摘Glucose detection in complex matrix such as physiological fluids and drinks can provide useful information guide for people.However,traditional detection methods toward complex matrix suffer from the impurity interference or complex pretreatments.So,it is important to exploit a universal and sensitive glucose detection strategy in complex matrix.In this work,a cascade catalytic scheme based on peroxidase-like MBs@MIL-100(Fe)@Ag(MMA)is developed for sensitive glucose detection in complex solution.Using 3,3’,5,5’-tetramethylbenzidine(TMB)as an indicator,MMA can trigger catalytic cascade reactions for specific glucose sensing.In particular,the peroxidase-like MIL-100(Fe)serves as both the catalysis unit and enrichment unit.Oxidation state of TMB(oxTMB)can be effectively and specifically enriched by MIL-100(Fe)to exclude the interference of undesired impurities and macromolecules,which is suitable for complex sample matrix including colored soda and saliva.In addition,utilizing the peroxidase-like activity of MIL-100(Fe)for self-clean,the residual indicator molecules can be degraded,resulting in the recyclable use of MMA. | Guohua Jiang Zhaoyan Yang Kai Zhu Shenfei Zong Lei Wu Zhuyuan Wang Yiping Cui | 2023 | Nano Research2023，16，1： | 0 |
| 12 | 2D profiling of tumor chemotactic and molecular phenotype at single cell resolution using a SERS-microfluidic chip显示文摘Emerging single-cell technologies create new opportunities for unraveling tumor heterogeneity.However,the development of high-content phenotyping platform is still at its infancy.Here,we develop a microfluidic chip for two-dimensional(2D)profiling of tumor chemotactic and molecular features at single cell resolution.Individual cells were captured by the triangular micropillar arrays in the cell-loading channel,facilitating downstream single-cell analysis.For 2D phenotyping,the chemotactic properties of tumor cells were visualized through cellular migratory behavior in microchannels,while their protein expression was profiled with multiplex surface enhanced Raman scattering(SERS)nanovectors,in which Raman reporter-embedded gold@silver core-shell nanoparticles(Au@Ag REPs)were modified with DNA aptamers targeting cellular surface proteins.As a proof of concept,breast cancer cells with diverse phenotypes were tested on the chip,demonstrating the capability of this platform for simultaneous chemotactic and molecular analysis.The chip is expected to provide a powerful tool for investigating tumor heterogeneity and promoting clinical precision medicine. | Yizhi Zhang Lei Wu Kuo Yang Shenfei Zong Zhuyuan Wang Yiping Cui | 2022 | Nano Research2022，15，5： | 0 |
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