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3篇 您的检索式:作者名="Sharshov KA"
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1Results of the influenza virus surveillance in wild birds in Western part of Mongolia显示文摘Objective:To present results of virological study of wild birds inhabiting Western Mongolia. Methods:Over a period of 2003-2008,we isolated 13 influenza A viruses:H1N1,H3N6, H13N8 and H4N6 subtypes.We did not isolate any H5N1 subtype,that still cause epizooty in wild birds and poultry.Results:We revealed taxonomic and ecological heterogeneity of the birds involved in maintenance of circulation of influenza viruses in the given territory.Influenza viruses were isolated from birds of 6 orders;among them there are species preferring water and semi-aquatic biotopes,one species preferring dry plain region,and also one species which can inhabit both dry and water biotopes.Conclusions:Representatives of all main orders of Western Mongolia avifauna are involved in support of influenza A virus circulation,highly pathogenic H5N1 influenza viruses were registered in Mongolia thus it’s necessary to continue permanent influenza virus surveillance in wild birds’ populations.Marchenko V Yu Alekseev A Yu Tserennorov D Yurlov AK Susloparov IM Sharshov KA Ilyinykh FA Zolotykh SI Abmed D Otgonbaatar D Shestopalov AM 2010Asian Pacific Journal of Tropical Medicine2010,3,2:1
2Ecology of influenza virus in wild bird populations in Central Asia显示文摘Marchenko VY Alekseev AY Sharshov KA 2012Avian Dis2012,56,1:1
3Detection of influenza A virus RNA in birds by optimized Real-Time PCR system显示文摘Objective:To evaluate the use of Real-Time PCR system based on specific amplification of matrix protein gene fragment for influenza A virus RNA detection in cloacal swabs from wild birds.Methods:Sensitivity,specificity and reproducibility of analysis results were identified. Study of cloacal swabs from wild birds for influenza A virus presence was performed.Results: Reproducibility of low concentrations of virus detection in samples by Real-Time PCR was significantly higher than that of detection based on cytopathic effect of viruses grown on MDCK cell culture.Conclusions:Real-Time PCR system for influenza A virus RNA detection is developed and applied for virus surveillance study.Ilinykh Ph A Shestopalova EM Khripko Yu I Durimanov AG Sharshov KA Shestopalov AM 2010Asian Pacific Journal of Tropical Medicine2010,3,5:0
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