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| 1 | Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma显示文摘AIM:To investigate cell type specific distribution ofβ-actin expression in gastric adenocarcinoma and its correlation with clinicopathological parameters.METHODS:β-actin is a housekeeping gene,frequently used as loading control,but,differentially expresses in cancer.In gastric cancer,an overall increased expression ofβ-actin has been reported using tissue disruptive techniques.At present,no histological data is available to indicate its cell type-specific expression and distribution pattern.In the present study,we analyzedβ-actin expression and distribution in paired normal and tumor tissue samples of gastric adenocarcinoma patients using immunohistochemistry(IHC),a tissue non-disruptive technique as well as tissue disruptive techniques like reverse transcriptase-polymerase chain reaction(RT-PCR)and western blotting.Correlation ofβ-actin level with clinicopathological parameters was done using univariate analysis.RESULTS:The results of this study showed significant overexpression,at both mRNA and protein level in tumor tissues as confirmed by RT-PCR(1.47±0.13 vs2.36±0.16;P<0.001)and western blotting(1.92±0.26 vs 2.88±0.32;P<0.01).IHC revealed thatβ-actin expression is majorly distributed between epithelial and inflammatory cells of the tissues.Inflammatory cells showed a significantly higher expression compared to epithelial cells in normal(2.46±0.13 vs 5.92±0.23,P<0.001),as well as,in tumor tissues(2.79±0.24 vs6.71±0.14,P<0.001).Further,comparison of immunostaining between normal and tumor tissues revealed that both epithelial and inflammatory cells overexpressβ-actin in tumor tissues,however,significant difference was observed only in inflammatory cells(5.92±0.23vs 6.71±0.14,P<0.01).Moreover,combined expression in epithelial and inflammatory cells also showed significant increase(4.19±0.15 vs 4.75±0.14,P<0.05)in tumor tissues.In addition,univariate analysis showed a positive correlation ofβ-actin level of inflammatory cells with tumor grade(P<0.05)while epithelial cells exhibited negative correlation(P>0.05).CONCLUSION:In gastric cancer,β-actin showed an overall higher expression predominantly contributed by inflammatory or tumor infiltrating immune cells of the tissue microenvironment and correlates with tumor grade. | Shafqat A Khan Monica Tyagi Ajit K Sharma Savio G Barreto Bhawna Sirohi Mukta Ramadwar Shailesh V Shrikhande Sanjay Gupta | 2014 | World Journal of Gastroenterology2014，20，34： | 2 |
| 2 | Total hip arthroplasty in fused hips with spine stiffness in ankylosing spondylitis显示文摘Ankylosing spondylitis(AS)is characterized by involvement of the spine and hip joints with progressive stiffness and loss of function.Functional impairment is significant,with spine and hip involvement,and is predominantly seen in the younger age group.Total hip arthroplasty(THA)for fused hips with stiff spines in AS results in considerable improvement of mobility and function.Spine stiffness associated with AS needs evaluation before THA.Preoperative assessment with lateral spine radiographs shows loss of lumbar lordosis.Spinopelvic mobility is reduced with change in sacral slope from sitting to standing less than 10 degrees conforming to the stiff pattern.Care should be taken to reduce acetabular component anteversion at THA in these fused hips,as the posterior pelvic tilt would increase the risk of posterior impingement and anterior dislocation.Fused hips require femoral neck osteotomy,true acetabular floor identification and restoration of the hip center with horizontal and vertical offset to achieve a good functional outcome.Cementless and cemented fixation have shown comparable long-term results with the choice dependent on bone stock at THA.Risks at THA in AS include intraoperative fractures,dislocation,heterotopic ossification,among others.There is significant improvement of functional scores and quality of life following THA in these deserving young individuals with fused hips and spine stiffness. | Anil Thomas Oommen Triplicane Dwarakanathan Hariharan Viruthipadavil John Chandy Pradeep MathewPoonnoose Arun Shankar A Roncy Savio Kuruvilla Jozy Timothy | 2021 | World Journal of Orthopedics2021，12，12： | 2 |
| 3 | Charaeterrisation of gastric Ghrelin cells in man and other mammals: syudies in adult and fetal tissues显示文摘 | Rindi G Necchi V Savio A | 2002 | Histochem Cell Biol2002，117，： | 1 |
| 4 | Steady state responses to multiple amplitude-modulated tones:an optimized method to test frequency-specific thresholds in hearing-impaired children and normal-hearing subjects显示文摘 | Perez-Abalo MC Savio G Torres A | 2001 | Ear Hear2001，22，3： | 1 |
| 5 | Liver X receptor (LXR)-beta regulation in LXR alpha-deficient mice: implications for therapeutic targeting显示文摘 | Quinet E M Savio D A Halpern A R | 2006 | Mol Pharmacol2006，70，4： | 1 |
| 6 | Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patients显示文摘 | Santos Savio A Machado Diaz AC Chico Capote A | 2015 | BMC Musculoskelet Disord2015，，： | 1 |
| 7 | Determination of chemical ox- ygen demand employed manganese as an environmentally friendly oxidizing reagent by a flow injection method based on microwave digestion and speeiation coupled to ICP -OES 显示文摘 | Almeida C A Savio M Gonzdlez P | 2013 | M ieroehemieal Journal: Devoted to the Application of Mieroteehniques in all Branches of Science2013，106，10： | 1 |
| 8 | Treatment of metastatic breast cancer with vinorelbine and docetaxel显示文摘 | Savio G Laudani A Leonardi V | 2006 | Am J Clin Oncol2006，29，3： | 1 |
| 9 | Soft-tissue aging and musculoskeletal function显示文摘 | Joseph A Buckwalter Savio LYWoo Victor MGoldberg | 1993 | The Jounal of Bone and Joint Surgery1993，75，10： | 1 |
| 10 | Diagnosis and posttreatment follow-up of Helicobacter pylori-positive -gastric lymphoma of mucosa-associated lymphoid tissue-histology, polymerase chain reaction, or both 显示文摘 | Savio A Franzia G Wotherspoon AL | 1996 | Blood1996，87，： | 1 |
| 11 | Diagnosis and posttreatment follow-up of helicobacter pylori-positive gastric lymphoma of mucosa-associated lymphoid tissue:histology,polymerase chain reaction,or both?显示文摘 | Franzin G Wotherspoon AC | 1996 | Blood1996，87，4： | 1 |
| 12 | Prognosticvalue of changes in resting-state functional connectivity pat-terns in cognitive recovery after stroke: A 3T fMRI pilotstudy 显示文摘 | Dacosta- Aguayo R Grana M Savio A | 2014 | Hum Brain Mapp2014，35，8： | 1 |
| 13 | Specific structural determinants are responsible for the an fioxidant activity and the cell cycle effects of resveratrol 显示文摘 | Stivala L A Savio M Carafoli F | 2001 | Biol Chem2001，276，22： | 1 |
| 14 | Antigenic mimicry between Helicobacter pylori and gastric mucosa in the pathogenesis of body atrophic gastritis显示文摘 | Negrini R Savio A Poiesi C | | 0，，： | 1 |
| 15 | Specific structural determinants are responsible for the antioxidant activity and the cell cycle effects of resveratrol显示文摘 | Stivala L A Savio M Carafoli F | 2001 | J Biol Chem2001，276，22： | 1 |
| 16 | Spe- cific Structural Determinants Are Responsible for the Antioxidant Activity and the Cell Cycle Effects of Res- veratrol显示文摘 | STIVALA L A SAVIO M CARAFOLI F | 2001 | Journal of Biological Chemistry2001，276，22： | 1 |
| 17 | Inhibition of mammalian DNA polymerases by Resveratrol:mechanism and structural determinants显示文摘 | Locatelli G A Savio M Forti L | 2005 | Biochem J2005，389，2： | 1 |
| 18 | Single Cell Gel Electrophore- sis(Comet) assay with plants: Research on DNA repair and ecogenotox- icity testing显示文摘 | Ventura L Giovannini A Savio M | 2013 | Chemosphere2013，92，1： | 1 |
| 19 | Multiple roles of the cell cycle inhibitor p21 (CDKN1A) in the DNA damage response显示文摘 | Cazzalini O Scovassi A I Savio M | 2010 | Mutat Res2010，704，13： | 1 |
| 20 | Steady state responses to multiple amplitude-modulated tones: an optimized method to test frequency-specific thresholds in hearing-impaired children and nomal-hearing subjects显示文摘 | Pere-Abalo MC Savio G Torres A | 2001 | Ear Hear2001，22，4： | 1 |
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