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| 1 | MicroRNAs expression influence in ulcerative colitis and Crohn's disease:A pilot study for the identification of diagnostic biomarkers显示文摘BACKGROUND Inflammatory bowel disease(IBD)comprises two distinct diseases,Crohn’s disease(CD)and ulcerative colitis(UC),both of which are chronic,relapsing inflammatory disorders of the gastrointestinal tract with a mostly unknown etiology.The incidence and prevalence of IBD are continually increasing,indicating the need for further studies to investigate the genetic determinants of these diseases.Since microRNAs(miRNAs)regulate protein translation via complementary binding to mRNA,discovering differentially expressed miRNAs(DE)in UC or CD patients could be important for diagnostic biomarker identification,assisting in the appropriate disease differentiation progressing the understanding of IBD pathogenesis.AIM To determine the miRNA expression profile in UC and CD patients and the potential pathophysiological contributions of differentially expressed miRNA.METHODS A total of 20 formalin-fixed paraffin-embedded colonic samples were collected from the Pathology Department of Botucatu Medical School at São Paulo State University(Unesp).The diagnosis of UC or CD was based on clinical,endoscopic,radiologic,and histological criteria and confirmed by histopathological analysis at the time of selection.The TaqMan™Array Human MicroRNA A+B Cards Set v3.0(Applied Biosystems™)platform was used to analyze 754 miRNAs.Targets of DE-miRNAs were predicted using miRNA Data Integration Portal(mirDIP)and the miRNA Target Interaction database(MiRTarBase).All statistical analyses were conducted using GraphPad Prism software.Parametric and nonparametric data were analyzed using t-tests and Mann-Whitney U tests,respectively.RESULTS The results showed that of the 754 miRNAs that were initially evaluated,643 miRNAs were found to be expressed in at least five of the patients who were diagnosed with either CD or UC;the remaining 111 miRNAs were not considered to be expressed in these patients.The expression levels of 28 miRNAs were significantly different between the CD and UC patients(P≤0.05);13 miRNAs demonstrated a fold-change in expression level greater than 1.Five miRNAs with a downregulated expression were selected for enrichment analysis.The miRNAs whose expression levels were significantly lower in UC patients than in CD patients were enriched in certain signaling pathways that were mostly correlated with cancer-related processes and respective biomarkers.CONCLUSION MiRNAs could be used to differentiate UC from CD,and differently expressed miRNAs could help explain the distinct pathophysiology of each disease. | Ana Elisa Valencise Quaglio Felipe Jose Santaella Maria Aparecida Marchesan Rodrigues Ligia Yukie Sassaki Luiz Claudio Di Stasi | 2021 | World Journal of Gastroenterology2021，27，45： | 3 |
| 2 | The influence of post-harvest UV-C hormesis on lycopene, β-carotene, and phenolic content and antioxidant activity of breaker tomatoes显示文摘 | Sergio Bravo Javier García-Alonso Gala Martín-Pozuelo Victoria Gómez Marina Santaella Inmaculada Navarro-González María Jesús Periago | 2012 | Food Research International2012，，1： | 2 |
| 3 | Application of a single-event kinetic model in the simulation of an industrial riser reactor for the catalytic cracking of vacuum gas oil显示文摘 | DEWACHTERE N V SANTAELLA F FROMENT G F | | 0，，15： | 1 |
| 4 | Comparison of oral nieotinamide adenine dinueleotide (NADH)versus conventional therapy forehronie fatigue syndrome显示文摘 | Santaella ML Font I Disdier OM | 2004 | P R Health Sei J2004，23，2： | 1 |
| 5 | Fluorinated phosphatidylcholine-based liposomes: H+/Na+ permeability,active doxorubicin encapsulation and stability, in human serum显示文摘 | Frezard F Santaella C Montisci MJ | 1994 | Biochim Biophys Acta1994，1194，1： | 1 |
| 6 | Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in 13-Ionone release 显示文摘 | Rubio-Moraga A Rambla J L Santaella M | 2008 | The Journal of Biological Chemistry2008，283，24： | 1 |
| 7 | The content and nutritional significance of minerals on fish flesh in the presence and absence of bone显示文摘 | MARTINEZ I JESUS M SANTAELLA M | 2000 | Food Chemistry2000，71，4： | 1 |
| 8 | Root border-like ceils of Arabidopsis:microscopical characterization and role in the interaction with rhizobacteria显示文摘 | VICRE' M SANTAELLA C BLANCHET S | 2005 | Plant Physiology2005，138，： | 1 |
| 9 | The use of thermochemical pretreatments to improve the anaerobic biodegradability and biochemical methane potential of the sugarcane ba- gasse 显示文摘 | Costa A G Pinheiro G C Pinheiro F G C Dos Santos A B Santaella S T Leitao R C | 2014 | Chemical Engineering Journal2014，248，： | 1 |
| 10 | The 1987 Mexican Disinflation Program: An Ex- change Rate Based Stabilization? 显示文摘 | Santaella J Vela A | 2005 | Economia Mexicana2005，，1： | 1 |
| 11 | Muscle tissue structural changes and texture development in sea bream (Sparus aurata L) during post-mortem storage显示文摘 | Ayala M D Abdel I Santaella M | 2010 | LWT-Food Science and Technology2010，43，3： | 1 |
| 12 | Application of a single-event kinetic model in the simulation of an industrial riser reactor for the catalytic cracking of vacuum gas oil 显示文摘 | DEWACHTERE N V SANTAELLA F FROMENT G F | 1999 | Chem Eng Sci1999，54，1516： | 1 |
| 13 | Application of asingle-event kinetic model in the simulation of an industrialriser reactor for the catalytic cracking of vacuum gas oil显示文摘 | Dewachtere N V Santaella F Froment G F | 1999 | Chemical Engineering Science1999，54，1516： | 1 |
| 14 | Application of a single-event kinetic model in the simulation of an industrial riser for the catalytic cracking of vacuum gas oil显示文摘 | Dewachtere N V Santaella F Froment G F | 1999 | Chem Eng Sci1999，54，1516： | 1 |
| 15 | Content and in vi- tro availability of Fe,Zn,Mg,Ca and P in homogenized fis- h-based weaning foods after bone addition显示文摘 | MARTINEZ I SANTAELLA M ROS G | 1998 | Food Chemistry1998，63，3： | 1 |
| 16 | Application of a single--event kinetic model in the simulation of an industrial riser for the catalytic cracking of vacuum gas oil 显示文摘 | Dewachtere N V Santaella F Froment G F | 1999 | Chem Eng Sci1999，54，1516： | 1 |
| 17 | Application of a single-event kinetic model in thesimulation of an industrial riser reactor for the catalyticcracking of vacuum gas oil显示文摘 | DEWACHTERE N V SANTAELLA F FROMENT G F | 1999 | Chemical EngineeringScience1999，54，1516： | 1 |
| 18 | Higher-order aberrations from the internal optics of the eye显示文摘 | Wang Li Santaella RM Booth M | | 0，，08： | 1 |
| 19 | Application of a single-event kinetic model in the simulation of an industrial riser reactor for the catalytic cracking of vacuum gas oil显示文摘 | Dewachtere N V Santaella F Froment G F | | 0，，15： | 1 |
| 20 | Oettlar adverse effects associated with systemic medications:recognition and management显示文摘 | Santaella RM Fraunfelder FW | 2007 | Drugs2007，67，1： | 1 |
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