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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | Reverse Logistics in E2busi2 ness显示文摘 | Samar K M Robert S | 2004 | International Journal of Physical Distribution & Logistics Management2004，34，1： | 1 |
| 4 | TMS-OTi-Catalyzed abromination of carbonyl compounds by N-bromosuccinimide 显示文摘 | Samar K G Bo W Beom S K | 2006 | Tetrahedron Letters2006，47，： | 1 |
| 5 | Arsenic exposure and adverse health effects: A review of recent findings from arsenic and health studies in Matlab, Bangladesh显示文摘 | Mohammad Y Nazmul S Samar K H | 2011 | The Kaohsiung Journal of Medical Sciences2011，27，9： | 1 |
| 6 | Aripiprazole in children and adolescents with Tourette's disorder:An open -label safety and tolerability study 显示文摘 | Lyon GJ Samar S Jummani R | 2009 | J Child Adolesc Psychopharmacol2009，19，6： | 1 |
| 7 | Synthesis and Structural Characterization of a Carboxylate Bridged Tetranuclear Copper Complex Derived from Reduced Schiff Base Asymmetric Compartmental Ligand Containing an Amino Acid side Arm显示文摘 | Chullikkattil Pradeep P Panthapally Zacharias S Samar Das K | 2006 | Inorganic Chemistry Communications2006，9，： | 1 |
| 8 | Renal amyloidosis:origin and clinicopathologic correlations of 474 recent cases显示文摘 | Samar MS Sanjeev S Anthony M | 2013 | Clin J Am Soc Nephrol2013，8，： | 1 |
| 9 | Reverse Logistics in E-business显示文摘 | Robert S | 2004 | International Journal of Physical Distribution & Logistics Management2004，34，1： | 1 |
| 10 | Link dynamics and protocol design in a multi-hop mobile environment显示文摘 | Samar P Wicker S B | | 0，，09： | 1 |
| 11 | Extraction of cobalt and lead from waste water using a liquid surfac- tant membrane emulsion显示文摘 | Bourenane S Samar M E H Abbaci A | 2003 | Acta Chimica Slovenica2003，50，4： | 1 |
| 12 | Variation in the reaction zone and its effects on the strength of diffusion bonded titanium-stainless steel couple显示文摘 | GHOSH M SAMAR D BANARJEE P S | 2005 | Materials Science and Engineering A2005，390，12： | 1 |
| 13 | Protective effects of anti-C5a peptide antibodies in experimental sepsis显示文摘 | Huber L M Samar M J Meguire S R | 2001 | FASEB J2001，15，： | 1 |
| 14 | Variation in the reaction zone and its effects on the strength of diffusion bonded titanium-stainless steel couple 显示文摘 | GHOSH M SAMAR D BANARJEE P S | 2005 | Mater Sci and Eng A2005，390，： | 1 |
| 15 | Metabolic effects of conjugated linoleic acid in humans:the Swedish experience显示文摘 | RlSéRUS U ANNIKA S SAMAR B | 2004 | Am J Clin Nutr2004，，： | 1 |
| 16 | Link dynamics and protocol design in a multihop mobile environment 显示文摘 | Samar P and Wicker S B | 2006 | IEEE Transactions on Mobile Computing2006，5，9： | 1 |
| 17 | A case of non - hyperammone- mic valproate - induced encephalopathy 显示文摘 | Deb SG Samar B Kartik S etal | 2006 | Neurol Asia2006，11，4： | 1 |
| 18 | Reverse Logistics in E - business Optimal Price and Return Policy 显示文摘 | SAMAR K M ROBERT S | 2004 | International Journal of Physical Distribution & Logistics Management2004，34，12： | 1 |
| 19 | Link dynamics and protocol design in a multihop mobile environment显示文摘 | Samar P Wicker S B | 2006 | IEEE Transactions on Mobile Computing2006，5，9： | 1 |
| 20 | Giant tonsillolith in a child显示文摘 | Joginder S G Virender S Samar Pal S Y | 2006 | International Journal of Pediatric Otorhinolaryngology Extra2006，1，： | 1 |
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