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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | A comparison of sevoflurane-propofol versus sevoflurane or propofol for laryngeal mask airway insertion in adults显示文摘 | Sahar MS Maie T Samar K | 2005 | Anesth Analg2005，100，5： | 1 |
| 5 | Clinical characteristics and treatment response in poor and good insight obsessive--com- pulsive disorder 显示文摘 | Ravi K V Samar R Janardhan R Y | 2004 | European Psychiatry2004，19，4： | 1 |
| 6 | Reverse Logistics in E2busi2 ness显示文摘 | Samar K M Robert S | 2004 | International Journal of Physical Distribution & Logistics Management2004，34，1： | 1 |
| 7 | TMS-OTi-Catalyzed abromination of carbonyl compounds by N-bromosuccinimide 显示文摘 | Samar K G Bo W Beom S K | 2006 | Tetrahedron Letters2006，47，： | 1 |
| 8 | Mukhopadhyay and Robert Setoputro: Reverse logistics in e-business optimal price and return policy 显示文摘 | Samar K | 2004 | Distribution & Logistics Management2004，34，1： | 1 |
| 9 | Arsenic exposure and adverse health effects: A review of recent findings from arsenic and health studies in Matlab, Bangladesh显示文摘 | Mohammad Y Nazmul S Samar K H | 2011 | The Kaohsiung Journal of Medical Sciences2011，27，9： | 1 |
| 10 | Incentives to reliable order fulfillment for an internet dropshipping supply chain显示文摘 | Yao Dong-qin Kurata Hisashi Mukhopadhyay Samar K | 2008 | Production Economics2008，113，1： | 1 |
| 11 | Synthesis and Structural Characterization of a Carboxylate Bridged Tetranuclear Copper Complex Derived from Reduced Schiff Base Asymmetric Compartmental Ligand Containing an Amino Acid side Arm显示文摘 | Chullikkattil Pradeep P Panthapally Zacharias S Samar Das K | 2006 | Inorganic Chemistry Communications2006，9，： | 1 |
| 12 | Tenancy Choiee in a Competitive Framework with Transaction Costs 显示文摘 | Alston J Samar K Jeffrey B N | 1984 | Journal of Political Economy1984，92，6： | 1 |
| 13 | Optimal return policy and modular design for build-to-order products显示文摘 | SAMAR K MUKHOPADHYAY ROBERT SETOPUTROL | 2005 | Journal of Operations Management2005，，23： | 1 |
| 14 | Joint procurement and production decisions in remanufacturing under quality and demand uncer- tainty 显示文摘 | Samar K Huafan M | 2009 | International Journ~ of Production Economics2009，120，1： | 1 |
| 15 | Nanocrystalline calcium phosphate ceramics in biomedical engineering显示文摘 | SAMAR J K ABHILASHA B HIMESH A B | 2007 | Materials Science and Engineering C2007，27，： | 1 |
| 16 | Joint procurement and production decisions in remanuIacturing under quality and demand uncertainty显示文摘 | Samar K Ma HF | 2009 | International Journal of Production Economics2009，120，1： | 1 |
| 17 | Reverse Logistics in E-business显示文摘 | Robert S | 2004 | International Journal of Physical Distribution & Logistics Management2004，34，1： | 1 |
| 18 | Role of C5a in multiorgan failure during sepsis显示文摘 | Huber L M Samar V J Lu K T | 2001 | J of Immunology2001，166，： | 1 |
| 19 | Microstructure and corrosion properties ofdiode laser melted friction stir weld of aluminum alloy2024-t351显示文摘 | SAMAR J K | 2011 | Applied Surface Science2011，257，9： | 1 |
| 20 | Tenancy Choice in a Competitive Framework with Transactions Costs 显示文摘 | Alston L Samar K Jeffey B N | 1984 | Journal of Political Economy1984，92，6： | 1 |
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