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| 1 | CRISPR/Cas9 gene editing and natural variation analysis demonstrate the potential for HvARE1 in improvement of nitrogen use efficiency in barley显示文摘Nitrogen is a major determinant of grain yield and quality.As excessive use of nitrogen fertilizer leads to environmental pollution and high production costs,improving nitrogen use efficiency(NUE)is fundamental for a sustainable agriculture.Here,we dissected the role of the barley abnormal cytokinin response1 repressor 1(Hv ARE1)gene,a candidate for involvement in NUE previously identified in a genome-wide association study,through natural variation analysis and clustered regularly interspacedshort palindromic repeats(CRISPR)/CRISPRassociated protein 9(Cas9)-mediated gene editing.Hv ARE1 was predominantly expressed in leaves and shoots,with very low expression in roots under low nitrogen conditions.Agrobacterium-mediated genetic transformation of immature embryos(cv.Golden Promise)with single guide RNAs targeting Hv ARE1 generated 22 T0 plants,from which four T1 lines harbored missense and/or frameshift mutations based on genotyping.Mutant are1 lines exhibited an increase in plant height,tiller number,grain protein content,and yield.Moreover,we observed a 1.5-to2.8-fold increase in total chlorophyll content in the flag leaf at the grain filling stage.Delayed senescence by 10–14 d was also observed in mutant lines.Barley are1 mutants had high nitrogen content in shoots under low nitrogen conditions.These findings demonstrate the potential of ARE1 in NUE improvement in barley. | Sakura D.Karunarathne Yong Han Xiao-Qi Zhang Chengdao Li | 2022 | Journal of Integrative Plant Biology2022，64，3： | 4 |
| 2 | Species-dependent effects of the phenolic herbicide ioxynil with potential thyroid hormone disrupting activity: modulation of its cellular uptake and activity by interaction with serum thyroid hormone-binding proteins显示文摘Ioxynil, a phenolic herbicide, is known to exert thyroid hormone (TH) disrupting activity by interfering with TH-binding to plasma proteins and a step of the cellular TH-signaling pathway in restricted animal species. However, comparative studies are still lacking on the TH disruption. We investigated the interaction of [125I] ioxynil with serum proteins from rainbow trout, bullfrog, chicken, pig, rat, and mouse, using native polyacrylamide gel electrophoresis. Candidate ioxynil-binding proteins, which included lipoproteins, albumin and transthyretin (TTR), differed among the vertebrates tested. Rainbow trout and bullfrog tadpole serum had the lowest binding activity for ioxynil, whereas the eutherian serum had the highest binding activity. The cellular uptake of, and response to, ioxynil were suppressed by rat serum greater than by tadpole serum. The cellular uptake of [125I]ioxynil competed strongly with phenols with a single ring, but not with THs. Our results suggested that ioxynil interferes with TH homeostasis in plasma and with a step of cellular TH-signaling pathway other than TH-uptake system, in a species-specific manner. | Sakura Akiyoshi Gobun Sai Kiyoshi Yamauchi | 2012 | Journal of Environmental Sciences2012，24，5： | 3 |
| 3 | Change in Expression of GBP28/Adiponectin in Carbon Tetrachloride-Administrated Mouse Liver显示文摘 | Madoka Yoda-Murakami Michiko Taniguchi Katsuhiko Takahashi Sakura Kawamata Kiyomi Saito Nam-Ho Choi-Miura Motowo Tomita | 2001 | Biochemical and Biophysical Research Communications2001，，： | 2 |
| 4 | The imbalance of plasminogen activators and inhibitor in prceclampsia 显示文摘 | Gao M Nakabayashi M Sakura M | 1996 | Obstetrics Gynccolo Res1996，22，1： | 1 |
| 5 | The characteristics of geochemistry in a headwater wetland,Chiba,Japan显示文摘 | Tang C Sakura Y | | 0，，294： | 1 |
| 6 | The comparative neurotoxicity of intrathecal lidocaine and bupivacaine in rats显示文摘 | Sakura S Kirihara Y Muguruma T | 2005 | Anesth Analg2005，101，2： | 1 |
| 7 | The effects of epidural anesthesia on ventilatory response to hypercapnia and hypoxia in elderly patients显示文摘 | Sakura S Saito Y Kosaka Y | 1996 | Anesth Analg1996，82，2： | 1 |
| 8 | The effect of pre-operative administration of midazolam on the development of intraoperative hypothemia显示文摘 | Toyota K Sakura S Saito Y | 2004 | Anaesthesia2004，59，2： | 1 |
| 9 | Comparison of 1% and 2% lidocaine epidural anaesthesia combined with sevoflu- rane general anaesthesia utilizing a constant bispectral in- dex 显示文摘 | Shono A Sakura S Saito Y | 2003 | Br J Anaesth2003，91，6： | 1 |
| 10 | Ultrasound guidedthoracic paravertebral block in breast surgery 显示文摘 | Hara K Sakura S Nomura T | 2009 | Anaes-thesia2009，64，2： | 1 |
| 11 | Progranulin protects against amyloid p deposition and toxicityin Alzheimer’s disease mouse models 显示文摘 | S Sakura Minami Min Sang-Won Grietj e Krabbe | 2014 | Nat Med2014，20，10： | 1 |
| 12 | Molecular character istics of GISTs显示文摘 | Sakura Y sakurai S | 2004 | Stomach Intestine(Tokyo)2004，39，4： | 1 |
| 13 | Automatic Sorting for Multi-Neuronal Activity Recorded With Tetrodes in the Presence of Overlapping Spikes显示文摘 | Susumu Takahashi Yuichiro Anzai Yoshio Sakura | 2003 | Journal of Neurophysiology2003，，89： | 1 |
| 14 | Comparative neurotoxicity of intrathecal and epidural lidocaine in rats显示文摘 | Kirihara Y Saito Y Sakura S | 2003 | Anesthesiology2003，99，： | 1 |
| 15 | Ultrasound-guided peripheral nerve blocks for anterior cruciate ligament reconstruction: effect of obturator nerve block during and after surgery 显示文摘 | SAKURA S HARA K OTA J | 2010 | J Anesth2010，24，3： | 1 |
| 16 | Comparative somatic and visceral antinociception and neurotoxicity of intrathecal bupivacaine, levobupivacaine, and dextrobupivacaine in rats 显示文摘 | MUGURUMA T SAKURA S KIRIHARA Y | 2006 | Anesthesiology2006，104，： | 1 |
| 17 | 90% write power-saving SRAM using sensing-amplifying memory cell 显示文摘 | Kanda K Sadaaki H Sakura T | 2004 | Journal of Solid State Circuit2004，39，6： | 1 |
| 18 | Nitrate pollution from agriculture in different hydrogeological zones of the regional groundwater flow system in the North China Plain 显示文摘 | Chert J Y Tang C Y Sakura S | 2005 | Hydrogeology Journal2005，13，： | 1 |
| 19 | Similarities and Varieties: A Brief Sketch on the Reception of Darwinism and Sociobiology in Japan显示文摘 | Osamu Sakura | 1998 | Biology and Philosophy1998，，3： | 1 |
| 20 | Haemodynamic effects of thoracic epidural anaesthesia during induction of anaesthesia:an investigation into the effects of tracheal intubation during targetcontrolled infusion of propofol显示文摘 | Nakatani T Saito Y Sakura S | 2005 | Anaesthesia2005，60，6： | 1 |
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