|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content,Alters Transcripts of Phytic Acid Biosynthetic Genes,and Modulates Mineral Distribution in Rice Seeds显示文摘Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway. | Aritra KARMAKAR Sananda BHATTACHARYA Shinjini SENGUPTA Nusrat ALI Sailendra Nath SARKAR Karabi DATTA Swapan K.DATTA | 2020 | Rice science2020，27，4： | 7 |
| 2 | The minimally invasive management of visceral artery aneurysms and pseudoaneurysms显示文摘 | Grant T. Fankhauser William M. Stone Sailendra G. Naidu Gustavo S. Oderich Joseph J. Ricotta Haraldur Bjarnason Samuel R. Money | 2011 | Journal of Vascular Surgery2011，，4： | 2 |
| 3 | Excitation requirements for stand alone three-phase induction generator显示文摘 | Chandan C Sailendra N B Ajit K C | 1998 | IEEE Trans on Energy Conversion1998，13，4： | 1 |
| 4 | Agrobacterium mediated genetic transformation of commercial jute cultivar Corchorus capsularis cv. JRC 321 using shoot tip explants显示文摘 | Prosanta Saha Karabi Datta Shuvobrata Majumder Chirabrata Sarkar Shyamsundar P. China Sailendra N. Sarkar Debabrata Sarkar Swapan K. Datta | 2014 | Plant Cell, Tissue and Organ Culture (PCTOC)2014，，2： | 1 |
| 5 | Successful coil embolization of circumflex lliac artery pseudoaneurysms following paracentesis显示文摘 | Day R W Huettl E A Sailendra G | 2014 | Vascular and Endovascular Surgery2014，48，23： | 1 |
| 6 | Damage in woven-fabric composites subjected to low-velocity impact显示文摘 | N.K. Naik Y. Chandra Sekher Sailendra Meduri | 2000 | Composites Science and Technology2000，，5： | 1 |
| 7 | Assessment of emergency escape routes for a building using Pathfinder-A Case Study显示文摘 | SAILENDRA D SHAH A | 2015 | International Journal of Engineering Sciences&Research Technology2015，4，6： | 1 |
| 8 | Damage in woven-fabric composites subjected to low-velocity impact 显示文摘 | Naik N K Chandra Sekher Y Meduri Sailendra | 2000 | Composites Science and Technology2000，60，： | 1 |
| 9 | The minimally invasive management of visceral artery aneurysms and pseudoaneurysms显示文摘 | Grant T. Fankhauser William M. Stone Sailendra G. Naidu Gustavo S. Oderich Joseph J. Ricotta Haraldur Bjarnason Samuel R. Money | 2011 | Journal of Vascular Surgery2011，，4： | 1 |
| 10 | 辛伐他汀对大鼠心肌缺血再灌注后细胞凋亡及线粒体融合素2表达的影响显示文摘目的:探讨辛伐他汀对大鼠心肌缺血再灌注后细胞凋亡及线粒体融合素2(Mitofusin 2,Mfn2)表达的影响。方法:选取32只Sprague-Dawley(SD)大鼠,随机分为假手术组、缺血再灌注模型组(模型组)、5 mg辛伐他汀组、10 mg辛伐他汀组,每组8只。5 mg辛伐他汀组、10 mg辛伐他汀组分别于术前7 d开始给予5 mg/(kg·d)、10 mg/(kg·d)的辛伐他汀灌胃,假手术组、模型组均给予等量蒸馏水灌胃。采用结扎左前降支法建立大鼠心肌缺血再灌注损伤模型。各组于造模后3 h剪取心脏组织,采用TUNEL法检测心肌细胞凋亡,Western Blot法检测p-Akt的表达,免疫组织化学法检测Mfn2的表达。结果 :与假手术组相比较,模型组心肌细胞凋亡指数、Mfn2的表达明显升高,p-Akt的表达显著降低(均P<0.05);与模型组比较,5 mg辛伐他汀组、10 mg辛伐他汀组心肌细胞凋亡指数、Mfn2的表达显著降低,p-Akt的表达显著升高(均P<0.05),并显示出明显剂量依赖性(P<0.05)。结论:辛伐他汀可有效抑制心肌缺血再灌注损伤后的心肌细胞凋亡,抑制Mfn2表达是其可能作用机制。 | Ajay Kumar Mishra 吴优 Shiv Kumar Yadav Akanand Singh Ram Narayan Mandal Sailendra Jha | 2016 | 南通大学学报(医学版)2016，36，4： | 0 |
| 11 | The Curation of Genetic Variants: Difficulties and Possible Solutions显示文摘The curation of genetic variants from biomedical articles is required for various clinical and research purposes. Nowadays, establishment of variant databases that include overall information about variants is becoming quite popular. These databases have immense utility, serving as a user-friendly information storehouse of variants for information seekers. While manual curation is the gold standard method for curation of variants, it can turn out to be time-consuming on a large scale thus necessitating the need for automation. Curation of variants described in biomedical literature may not be straightforward mainly due to various nomenclature and expression issues. Though current trends in paper writing on variants is inclined to the standard nomenclature such that variants can easily be retrieved, we have a massive store of variants in the literature that are present as non-standard names and the online search engines that are predominantly used may not be capable of finding them. For effective curation of variants, knowledge about the overall process of curation, nature and types of difficulties in curation, and ways to tackle the difficulties during the task are crucial. Only by effective curation, can variants be correctly interpreted. This paper presents the process and difficulties of curation of genetic variants with possible solutions and suggestions from our work experience in the field including literature support. The paper also highlights aspects of interpretation of genetic variants and the importance of writing papers on variants following standard and retrievable methods. | Kapil Raj Pandey Narendra Maden Barsha Poudel Sailendra Pradhananga Amit Kumar Sharma | 2012 | Genomics, Proteomics & Bioinformatics2012，10，6： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员