|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity显示文摘Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata(U. lobata) through dipeptidyl peptidase IV(DPP-IV) inhibitory activity.Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPPIV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPPIV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 μg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol. | Yudi Purnomo Djoko Wahono Soeatmadji Sutiman Bambang Sumitro Mochammad Aris Widodo | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，8： | 2 |
| 2 | True-Stress Measurement of PC Steels by EM sensor 显示文摘 | Sumitro S | 2001 | J of Pre-Stressed Concrete Japan(Japan Prestressed Concrete Engineering Association)2001，43，6： | 1 |
| 3 | Sustainable structural health monitoring system显示文摘 | Sumitro S Wang M L | 2005 | Structural Control and Health Monitoring2005，12，34： | 1 |
| 4 | Long span bridge health monitoring system in Japan 显示文摘 | Sunaryo Sumitro Yoshimasa Matsui | 2001 | Proceedings of SPIE2001，，4337： | 1 |
| 5 | Elasto-magnetic sensor utilization on steel cable stress measurement显示文摘 | SUMITRO S JAROSEVIC A WANG M L | 2004 | Monitoring2004，15，： | 1 |
| 6 | True-stress measurement of PC steels by EM sensor,Joural of Pre-stressed Concrete Japan显示文摘 | | 2001 | Japan Prestressed Concrete Engineering Association2001，43，6： | 1 |
| 7 | Long span bridge health monitoring system in Japan显示文摘 | Sunaryo Sumitro Yoshimasa Matsui | 2001 | Proceedings of SPIE2001，，4337： | 1 |
| 8 | True-stress measurement of PC steels by EM sensor显示文摘 | SUMITRO S | 2001 | Journal of Prestressed Concrete Japan (JPCEA)2001，43，6： | 1 |
| 9 | Monitoring based maintenance utilizing actual stress sensory technology显示文摘 | SUMITRO S KUROKAWA S SHIMANO K | 2005 | Smart Materials and Structures2005，14，3： | 1 |
| 10 | Elasto-Magnetic Sensor Unilization on Steel Cable Stress Measurement显示文摘 | Sunaryo Sumitro Andrej Jarosevic Ming L WANG | 2004 | Monitoring Session2004，15，： | 1 |
| 11 | Monitoring based maintenance utilizing actual stress sensory technology 显示文摘 | SUNARYO SUMITRO | 2005 | Smart Materials and Structures2005，14，： | 1 |
| 12 | Sustainable structural health monitoring system显示文摘 | Sumitro S1 Wang ML | 2005 | Structural Control and Health Monitoring2005，3，12： | 1 |
| 13 | Sustainable structure health monitoring system 显示文摘 | SUNARYO SUMITRO | 2005 | Structure Control and Health Monitoring2005，，12： | 1 |
| 14 | Elasto-magnetic sensor utilization on steel cable stress measurement显示文摘 | SUMITRO S JAROSEVIC A WANG M L | | 0，，： | 1 |
| 15 | Elasto-magnetic sensor utilization on steel cable stress measurement显示文摘 | SUMITRO S JAROSEVIC A WANG M L | 2004 | Monitoring Session 152004，，： | 1 |
| 16 | Monitoring based maintenance utilizing actual stress sensory technology显示文摘 | Sunaryo Sumitro | 2005 | Smart Materials and Structures2005，，14： | 1 |
| 17 | Sustainable structure health monitoring system显示文摘 | Sunaryo Sumitro | 2005 | Structure Control and Health Monitoring2005，，12： | 1 |
| 18 | True-Stress Measurement of PC Steels by EM Sensor显示文摘 | Sumitro S | 2001 | Journal of Pre-Stressed Concrete Japan (Japan Prestressed Concrete Engineering Association) Nov2001，43，6： | 1 |
| 19 | Decreasing a-synuclein aggregation by methanolic extract of Centella asiatica in zebrafish Parkinson's model显示文摘Objective: To observe the effects of Centella asiatica(C. asiatica) methanolic extract on a-synuclein aggregation and its expression in rotenone-exposed zebra fish.Methods: Zebra fish(Danio rerio) were exposed to 5 m g/L rotenone for 28 days and coincubated with 2.5, 5.0 and 10.0 m g/mL of C. asiatica methanolic extract. The medium was changed every 48 h for maintain the concentration of rotenone and extract. After 28 days zebra fish were sacrificed on the ice block and protein was isolated from zebra fish brain for ELISA of dopamine and Western blotting of a-synuclein. Immunohistochemistry was conducted to observe the a-synuclein expressions from histopathological preparation of zebra fish brain. The head were soaked in 10% formaline for less than 24 h and embedded onto paraffin block, then sliced for immunohistochemistry using anti a-synuclein antibody. We also measured zebra fish motility for 5 min in each week.Results: C. asiatica has important bioactive compounds such as asiaticoside that has antiin flammatory and antioxidant properties. It may inhibit cascade reaction due to oxidative stress induced by rotenone. Decreasing reactive oxygen species proposed probability of radical attack to a-synuclein protein that caused aggregation and increase of its expression.The motility of zebra fish was also maintained in C. asiatica groups due to the increasing dopamine level in rotenone-induced zebra fish. High level of reactive oxygen species inactivated enzyme for dopamine synthesis such as tyrosine hydroxylase, and oxidized dopamine itself. Oxidized dopamine increased a-synuclein aggregation. Thus, the dopamine level decreased in rotenone-induced zebra fish, but C. asiatica increased dopamine level.Conclusions: C. asiatica has a potential to be developed as an anti-Parkinson's disease treatment due to its capability for minimized the sign of Parkinson's such as a-synuclein aggregation and expression, increasing motility and dopamine as well. | Husnul Khotimah Mulyohadi Ali Sutiman Bambang Sumitro Mochamad Aris Widodo | 2015 | Asian Pacific Journal of Tropical Biomedicine2015，5，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员